ID   PSIT1_PSICU             Reviewed;         471 AA.
AC   P0DPB1;
DT   25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2017, sequence version 1.
DT   25-MAY-2022, entry version 12.
DE   RecName: Full=Major facilitator-type transporter psiT1 {ECO:0000303|PubMed:28763571};
DE   AltName: Full=Psilocybin biosynthesis cluster transporter 1 {ECO:0000303|PubMed:28763571};
GN   Name=psiT1 {ECO:0000303|PubMed:28763571};
OS   Psilocybe cubensis (Psychedelic mushroom) (Stropharia cubensis).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Agaricomycetidae; Agaricales; Strophariaceae; Psilocybe.
OX   NCBI_TaxID=181762;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION, AND FUNCTION.
RX   PubMed=28763571; DOI=10.1002/anie.201705489;
RA   Fricke J., Blei F., Hoffmeister D.;
RT   "Enzymatic synthesis of psilocybin.";
RL   Angew. Chem. Int. Ed. 56:12352-12355(2017).
CC   -!- FUNCTION: Major facilitator-type transporter; part of the gene cluster
CC       that mediates the biosynthesis of psilocybin, a psychotropic
CC       tryptamine-derived natural product (PubMed:28763571).
CC       {ECO:0000305|PubMed:28763571}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. TCR/Tet
CC       family. {ECO:0000305}.
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DR   EMBL; MF000991; ASU62244.1; -; Genomic_RNA.
DR   AlphaFoldDB; P0DPB1; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
PE   3: Inferred from homology;
KW   Glycoprotein; Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..471
FT                   /note="Major facilitator-type transporter psiT1"
FT                   /id="PRO_0000442162"
FT   TRANSMEM        44..64
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        108..128
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        140..160
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        168..188
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        212..232
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        237..257
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        322..342
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        356..376
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        424..444
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..36
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        25
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        384
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   471 AA;  51791 MW;  511738BBBA9F7A05 CRC64;
     MNPTTATDAH ERTSLLSGRP QSAANSTAPY ERQVQPSRKS QCFTPVTVIT IITLIYRLAT
     TMVITTNIRV LHTVACQLWY HVNDPDVFPG GNIPEKYCAL PGVDKYYAIM VSMTTVIDGL
     GGILGTGIAS YMSSRFGRKP VLMFLLSCTM IDHLAILTVQ NVYGWKQLVT FGLIMIVETI
     GNENTTVFLV SMYVVDVTEA ERRTAALSSI TGWLVLGGAL AYSIGGSITT FLHSNSAVYI
     VSFSVTGIVL TFTAFVLPES FPAEKRDLLR LERLAETRGH SQSWTQKIKA VATVALEPME
     LLKPTFNPIT GKANWRLVYC ALHSFIVTLA DAYALPAMLI FFTTQYSYTP AQMGYVMTTY
     SVSSVFVLAI ALPLFIRWFK PLYNNTQTKS VPDEGDGLRA TDSGEAGVHT QEVVVSETSD
     RMDVHITVIS WTIESLAYIV LGTVGSFYAQ LLGRPLPLLA LDLDAFQEFE A