ID   PSIT2_PSICU             Reviewed;         523 AA.
AC   P0DPB2;
DT   25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT   25-OCT-2017, sequence version 1.
DT   25-MAY-2022, entry version 12.
DE   RecName: Full=Major facilitator-type transporter psiT2 {ECO:0000303|PubMed:28763571};
DE   AltName: Full=Psilocybin biosynthesis cluster transporter 2 {ECO:0000303|PubMed:28763571};
GN   Name=psiT2 {ECO:0000303|PubMed:28763571};
OS   Psilocybe cubensis (Psychedelic mushroom) (Stropharia cubensis).
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Agaricomycetidae; Agaricales; Strophariaceae; Psilocybe.
OX   NCBI_TaxID=181762;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION, AND FUNCTION.
RX   PubMed=28763571; DOI=10.1002/anie.201705489;
RA   Fricke J., Blei F., Hoffmeister D.;
RT   "Enzymatic synthesis of psilocybin.";
RL   Angew. Chem. Int. Ed. 56:12352-12355(2017).
CC   -!- FUNCTION: Major facilitator-type transporter; part of the gene cluster
CC       that mediates the biosynthesis of psilocybin, a psychotropic
CC       tryptamine-derived natural product (PubMed:28763571).
CC       {ECO:0000305|PubMed:28763571}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. TCR/Tet
CC       family. {ECO:0000305}.
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DR   EMBL; MF000992; ASU62245.1; -; Genomic_RNA.
DR   AlphaFoldDB; P0DPB2; -.
DR   SMR; P0DPB2; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   InterPro; IPR005829; Sugar_transporter_CS.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
PE   3: Inferred from homology;
KW   Glycoprotein; Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..523
FT                   /note="Major facilitator-type transporter psiT2"
FT                   /id="PRO_0000442163"
FT   TRANSMEM        88..108
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        125..145
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        149..169
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        175..195
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        221..241
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        317..337
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        352..372
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        382..402
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        419..439
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        455..474
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        488..508
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..45
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1..42
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CARBOHYD        269
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        410
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   523 AA;  56907 MW;  764B5F38354E7E78 CRC64;
     MSLERSTSPN PTERTSLLSD TASTISSRDD VEQSSLKQRR TPIPTGQLGG KVSMHSIIIN
     AEGHLWPYIN QFVNDIGVSD GNPRNVGFYS GLIESVFACG EVCSIFMLSR LSDRIGRRPV
     LLPSALGIAV FTALFGLSSS FTMMLTLRVC AGLLAGATPI VHSIVSELTD DTNNALVVPL
     YGLITPIGFA IGPLIGGTLE HAATKYPNVF GYELFRKYPY FLPSFVPCCM AIVGVTFGYF
     FLKETLPSLV KSKKRLERQR SSSSISSENS TLYGATEHIR DSTEETAADE EPDSKPKGIT
     ELIRDPSIRA IMASGTFLMF LYTSSDVIFS LYCFTAVEDG GVGLPPEKIG YAFSVAGLIA
     MLMQLCITPW VLRTFDKAKV YHFCMCSFPL VFALMGCLNP LAQTGYSEIN KTLHPTTTGL
     LYAAIAILLL LARVCVMAFP ISMMLVKQTA DKHSLATANG LVQVAMTLAR AFCPTISSSV
     FAYSTSHNIL GGHFWVVVMV FISLVGVWQS TKIARVTKTK EQL