ID   PSIT2_PSICY             Reviewed;         528 AA.
AC   A0A286LF01;
DT   05-DEC-2018, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2017, sequence version 1.
DT   25-MAY-2022, entry version 12.
DE   RecName: Full=Major facilitator-type transporter psiT2 {ECO:0000303|PubMed:28763571};
DE   AltName: Full=Psilocybin biosynthesis cluster transporter 2 {ECO:0000303|PubMed:28763571};
GN   Name=psiT2 {ECO:0000303|PubMed:28763571};
OS   Psilocybe cyanescens.
OC   Eukaryota; Fungi; Dikarya; Basidiomycota; Agaricomycotina; Agaricomycetes;
OC   Agaricomycetidae; Agaricales; Strophariaceae; Psilocybe.
OX   NCBI_TaxID=93625;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION, AND FUNCTION.
RC   STRAIN=FSU 12416;
RX   PubMed=28763571; DOI=10.1002/anie.201705489;
RA   Fricke J., Blei F., Hoffmeister D.;
RT   "Enzymatic synthesis of psilocybin.";
RL   Angew. Chem. Int. Ed. 56:12352-12355(2017).
CC   -!- FUNCTION: Major facilitator-type transporter; part of the gene cluster
CC       that mediates the biosynthesis of psilocybin, a psychotropic
CC       tryptamine-derived natural product. {ECO:0000305|PubMed:28763571}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the major facilitator superfamily. TCR/Tet
CC       family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; MF000996; ASU62249.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A286LF01; -.
DR   SMR; A0A286LF01; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   Gene3D; 1.20.1250.20; -; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   InterPro; IPR005829; Sugar_transporter_CS.
DR   Pfam; PF07690; MFS_1; 1.
DR   SUPFAM; SSF103473; SSF103473; 1.
DR   PROSITE; PS50850; MFS; 1.
DR   PROSITE; PS00216; SUGAR_TRANSPORT_1; 1.
PE   2: Evidence at transcript level;
KW   Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..528
FT                   /note="Major facilitator-type transporter psiT2"
FT                   /id="PRO_0000445832"
FT   TRANSMEM        87..107
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        125..145
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        148..168
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        174..194
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        220..240
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        322..342
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        357..377
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        388..408
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        424..444
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        460..479
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        493..513
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..20
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          260..299
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        261..276
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        277..299
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   528 AA;  57540 MW;  B20C4CAAFBCE923B CRC64;
     MSPERSASLE PDEHSSLLSD TASYISRDDL EDSKAKQIPT PIPKKQLGVL FSIRFTEPII
     YSHLWPYINQ FVNDIGVADG NPRYVGFYSG LIESVFACGE VCSIFMLSRL SDRIGRRPVL
     LPSALGVALF TALFGLSTSF TMMLVLRVCA GLLAGATPIV HSVVSELTDE TNNALVVPLY
     GLITPIGFAI GPLIGGTLEH AATKYPNVFG YDFLRKYPYF LPSFVPCCLA VVGVTFGYFF
     LQETLPSIVR AKKRLERQKS TSSISSRTST LYGATDDHNR DASESTALSP EEAEDEIDSK
     PQSIKALIVD PSMRAIMGSG TFLMFLYTSS DVLFSLYCFT AVEDGGVGLP PDEIGYAFSV
     AGVIAMLMQL CITPWVLRTF DKAKVYKFCM FSFPLVFALM GCLNPLAQTG YNEVSKTIHP
     TTTGLLYAAI AVLLLLARVC VMAFPISMML IKQNADKNSL ATANGLVQVS MTIARALCPT
     VSSSLFAYST SNNILGGHLW VLIMVTISLA GVWQSMSIAR VTKRKEEL