ID   PSK_DAUCA               Reviewed;           5 AA.
AC   P58261;
DT   29-AUG-2001, integrated into UniProtKB/Swiss-Prot.
DT   29-AUG-2001, sequence version 1.
DT   11-DEC-2019, entry version 41.
DE   RecName: Full=Phytosulfokine-alpha;
DE            Short=PSK-alpha;
DE   Contains:
DE     RecName: Full=Phytosulfokine-beta;
DE              Short=PSK-beta;
OS   Daucus carota (Wild carrot).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Apiales; Apiaceae; Apioideae; Scandiceae; Daucinae;
OC   Daucus; Daucus sect. Daucus.
OX   NCBI_TaxID=4039;
RN   [1]
RP   PROTEIN SEQUENCE, SULFATION AT TYR-1 AND TYR-3, AND IDENTIFICATION BY MASS
RP   SPECTROMETRY.
RC   STRAIN=cv. US-Harumakigosun;
RX   PubMed=10750705; DOI=10.1093/pcp/41.1.27;
RA   Hanai H., Matsuno T., Yamamoto M., Matsubayashi Y., Kobayashi T.,
RA   Kamada H., Sakagami Y.;
RT   "A secreted peptide growth factor, phytosulfokine, acting as a stimulatory
RT   factor of carrot somatic embryo formation.";
RL   Plant Cell Physiol. 41:27-32(2000).
CC   -!- FUNCTION: In presence of 2,4-D, stimulates proliferation of the cells,
CC       but does not stimulate differentiation into the somatic embryos.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- PTM: Sulfation is important for activity and for the binding to a
CC       putative membrane receptor. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the phytosulfokine family. {ECO:0000305}.
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DR   PDB; 4Z5W; X-ray; 2.20 A; P/Q=1-5.
DR   PDB; 4Z61; X-ray; 2.75 A; P/Q=1-5.
DR   PDB; 4Z63; X-ray; 2.51 A; P=1-5.
DR   PDB; 4Z64; X-ray; 2.66 A; P=1-5.
DR   PDBsum; 4Z5W; -.
DR   PDBsum; 4Z61; -.
DR   PDBsum; 4Z63; -.
DR   PDBsum; 4Z64; -.
DR   SMR; P58261; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008083; F:growth factor activity; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Growth factor; Secreted;
KW   Sulfation.
FT   PEPTIDE         1..5
FT                   /note="Phytosulfokine-alpha"
FT                   /id="PRO_0000045865"
FT   PEPTIDE         1..4
FT                   /note="Phytosulfokine-beta"
FT                   /id="PRO_0000024060"
FT   MOD_RES         1
FT                   /note="Sulfotyrosine"
FT                   /evidence="ECO:0000269|PubMed:10750705"
FT   MOD_RES         3
FT                   /note="Sulfotyrosine"
FT                   /evidence="ECO:0000269|PubMed:10750705"
SQ   SEQUENCE   5 AA;  687 MW;  76C1BB504B300000 CRC64;
     YIYTQ