PSLA_DICDI
ID PSLA_DICDI Reviewed; 1272 AA.
AC Q55FB8; O43993;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 61.
DE RecName: Full=Presporeless protein A;
GN Name=pslA; ORFNames=DDB_G0267410;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 4-1272, FUNCTION IN MORPHOGENESIS,
RP SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RC STRAIN=AX3-1;
RX PubMed=9636072; DOI=10.1242/dev.125.14.2565;
RA Yasukawa H., Mohanty S., Firtel R.A.;
RT "Identification and analysis of a gene that is essential for morphogenesis
RT and prespore cell differentiation in Dictyostelium.";
RL Development 125:2565-2576(1998).
CC -!- FUNCTION: Functions autonomously, very early in the prespore pathway,
CC to control prespore cell differentiation, maybe at the level of
CC transcription. Also required for proper aggregation.
CC {ECO:0000269|PubMed:9636072}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:9636072}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout growth and development with a
CC strong peak of expression at 4 hours when aggregation is initiating.
CC {ECO:0000269|PubMed:9636072}.
CC -!- DISRUPTION PHENOTYPE: Cells produce large aggregation streams under
CC conditions in which wild-type cells form discrete aggregates. They are
CC unable to induce prespore differentiation, do not express prespore-
CC specific genes and do not produce mature spores. The vast majority of
CC these cells participate in multicellular development and preferentially
CC localize to the very anterior of the slug and form the prestalk and
CC stalk components. {ECO:0000269|PubMed:9636072}.
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DR EMBL; AAFI02000003; EAL73157.1; -; Genomic_DNA.
DR EMBL; AF038919; AAB95479.1; -; Genomic_DNA.
DR PIR; T14476; T14476.
DR RefSeq; XP_647615.1; XM_642523.1.
DR AlphaFoldDB; Q55FB8; -.
DR SMR; Q55FB8; -.
DR STRING; 44689.DDB0191506; -.
DR PaxDb; Q55FB8; -.
DR EnsemblProtists; EAL73157; EAL73157; DDB_G0267410.
DR GeneID; 8616427; -.
DR KEGG; ddi:DDB_G0267410; -.
DR dictyBase; DDB_G0267410; pslA.
DR HOGENOM; CLU_263868_0_0_1; -.
DR PRO; PR:Q55FB8; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0005634; C:nucleus; IDA:dictyBase.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IMP:dictyBase.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Sporulation.
FT CHAIN 1..1272
FT /note="Presporeless protein A"
FT /id="PRO_0000327755"
FT REGION 369..403
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 468..490
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 146..161
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 373..390
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 468..485
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1272 AA; 148935 MW; 92F6009EBBA50A2D CRC64;
MGRMKVIPEF SNYKIRTPIP IFKNELEIEK YIKGAISYIY SENTAKKLEL KKRNDEIKEI
LNNKNDHEFV EKKIRAIVIQ LGDTNLNDRC YLNYLNLYKQ PIEFTHGAIM CEKNKYWVAY
YASQSLNLDC LNFIIKHKDQ FKGLDKDKRI KPIDPKKKIS RDLLIGQPIT VQCIKEDDLF
KFLTVYFNQN GIEFSDSWFS SIVKLIFKGK VYSSDESEKL STTTTTTTTP NEKYDKKILG
EHTVFINKNR DGHLKFSTIP SLLLKISTLM NTGVINEYKQ KNYFLETLYN SFPANKHFFN
FNEETIQYIT NLLLPHLHPL EFKRFNTIPW IIKSKEFIDF IIQHKDFYHL VPSIHKFTKK
HENTNLFSAS TINDGEEEDD DDDDNDVDGN DDDNNKEKVD DTSNKKDSIV KFKDDITIYL
NVRQRFLQFS NFELANYFHT KLLECQKESE NQINSNIQSI NKEIESLSTS STNTASSTRS
KASSNSNQLK KKEDLEQQII ELQSLLKNKY FSNKEIYYLS FLRALKDCDI STIESIDKVV
SIDSDQNNNK FQILFQKSFL ENQPLKNGIS PEFYDFKKVG PSSHPDVYAE GEYDEHDEYD
DYQGKLDNHI DIKDSKFTKD ENSRDQLFNY LKSSGFKSFT PSIFCYLLEL LLLINTEKSN
QQFQEIFKPI STTTTTSTTT TTTTTNLDNI NEKIFKLSSD LLIHLVHIVD FKKFSIILDS
VNLNRKLILE MIDNCKNKLS FQKRIYEFHD VRSKDYQYDI NGHFSTNIKF SDAKDILKKL
IDENLFPTSK DWIFIYHSLY ISILQSTGVT LNNLLEVNKI YEEHEINYPS VLPNHSNYIL
DFNNNNNNNN NNNYYGLLNY QPYNNSGNIN NESLKNVVSN FVLFYWFHLL DRKLSKLFVR
SFSNSNFNEF SSSRLPCLKN SSICRIRSKR HSNTASQIFK SIYELQNFSL LEKYSTFKSY
FPEERSYYYN NPSDPFYHPD GSKDFRVLIE EGEFQLAFNH LQLVATKTVS EFPPLTTSRL
FQFITLEDVI ELINLTTIKN FDESKDESEQ QPLLKMWYGK DVKKCKDWIL SCAIAKSRLD
IVDLLLVKDI EYSTLPSTIE FLTGYKIIKS LFSPECDNQT LEYFLTFSNG IVLPSIKQYL
IKDNIATQTK NDVFRVIRHG IGKFELLRTF IPSLGFSSCL IEKMVENRRF ETLQYYMEIG
LITNEDLTNQ QKDQLKYEND LKHLDWVINL NHKKRVNRNQ PTFTPNTNST TATTATPLLQ
TRSGRTIIPI KK
