ID   PSMD3_DAUCA             Reviewed;         489 AA.
AC   Q06364;
DT   15-DEC-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 2.
DT   02-JUN-2021, entry version 88.
DE   RecName: Full=Probable 26S proteasome non-ATPase regulatory subunit 3;
DE            Short=26S proteasome subunit S3;
DE   AltName: Full=26S proteasome regulatory subunit RPN3;
DE   AltName: Full=Nuclear antigen 21D7;
GN   Name=21D7;
OS   Daucus carota (Wild carrot).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Apiales; Apiaceae; Apioideae; Scandiceae; Daucinae;
OC   Daucus; Daucus sect. Daucus.
OX   NCBI_TaxID=4039;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   PubMed=8310059; DOI=10.1104/pp.101.3.809;
RA   Smith M.W., Ito M., Yamada T., Suzuki T., Komamine A.;
RT   "Isolation and characterization of a cDNA clone for plant nuclear antigen
RT   21D7 associated with cell division.";
RL   Plant Physiol. 101:809-817(1993).
RN   [2]
RP   CHARACTERIZATION.
RX   PubMed=9008397; DOI=10.1104/pp.113.1.281;
RA   Smith M.W., Ito M., Miyawaki M., Sato S., Yoshikawa Y., Wada S., Maki H.,
RA   Nakagawa H., Komamine A.;
RT   "Plant 21D7 protein, a nuclear antigen associated with cell division, is a
RT   component of the 26S proteasome.";
RL   Plant Physiol. 113:281-291(1997).
CC   -!- FUNCTION: Acts as a regulatory subunit of the 26 proteasome which is
CC       involved in the ATP-dependent degradation of ubiquitinated proteins.
CC   -!- SUBUNIT: The 26S proteasome is composed of a core protease, known as
CC       the 20S proteasome, capped at one or both ends by the 19S regulatory
CC       complex (RC). The RC is composed of at least 18 different subunits in
CC       two subcomplexes, the base and the lid, which form the portions
CC       proximal and distal to the 20S proteolytic core, respectively (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- SIMILARITY: Belongs to the proteasome subunit S3 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAA02696.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; D13434; BAA02696.1; ALT_FRAME; mRNA.
DR   PIR; JQ2257; JQ2257.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0000502; C:proteasome complex; IEA:UniProtKB-KW.
DR   GO; GO:0030234; F:enzyme regulator activity; IEA:InterPro.
DR   GO; GO:0042176; P:regulation of protein catabolic process; IEA:InterPro.
DR   InterPro; IPR013586; 26S_Psome_reg_C.
DR   InterPro; IPR000717; PCI_dom.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF01399; PCI; 1.
DR   Pfam; PF08375; Rpn3_C; 1.
DR   SMART; SM00088; PINT; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS50250; PCI; 1.
PE   1: Evidence at protein level;
KW   Nucleus; Proteasome.
FT   CHAIN           1..489
FT                   /note="Probable 26S proteasome non-ATPase regulatory
FT                   subunit 3"
FT                   /id="PRO_0000173824"
FT   DOMAIN          241..422
FT                   /note="PCI"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01185"
FT   REGION          1..23
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          454..489
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        454..482
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   489 AA;  55529 MW;  F9668CC2D75B8D71 CRC64;
     MTQDVEMKEV PAPAPSNSVT AATPSTLQHL KEIASLIESG AYAREVRRIL RAVRLTIALR
     KKLNASVVNA FLNFSLVPGS EVHARLASYL PKEDEHDMEV DTAMSATTTL AKHSLPELEI
     YCYLLVLIFL IDQKKYSEAK ACSSASIARV KNLNRRTVEV LASRLYFYYS LSYELTGDLA
     EIRGNLLALH RIATLRHDEL GQETLLNLLL RNYLHYNLYD QAEKLRSKAP RFEAHSNQQF
     CRYLFYLGKI RTIQLEYTDA KESXLQAARK APVAALGFRV QCNKWAVIVR LLLGEIPERT
     VFMQKGMEKA LRPYFXLTNA VRIGDLELFR XVADKFASTF TADRTHNLIV RLRHNVIRTG
     LRNISISYSR ISLVDVARKL RLDSPNPVAD AESIVSKAIR DGAIDATIDH ANGWMVSKET
     GDIYSTNEPQ AAFNSRIAFC LNMHNEAVRA LRFPANSHKD KESAEKRRER QQQEQELAKH
     IAEEDDDEF