PSMD8_DICDI
ID PSMD8_DICDI Reviewed; 263 AA.
AC P02889; P09409; Q556L0; Q86JZ9;
DT 21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT 14-NOV-2003, sequence version 2.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Probable 26S proteasome non-ATPase regulatory subunit 8;
DE AltName: Full=26S proteasome regulatory subunit RPN12;
DE AltName: Full=26S proteasome regulatory subunit S14;
DE AltName: Full=Protein M4;
DE AltName: Full=Vegetative cell protein X;
GN Name=psmD8-1; Synonyms=M4; ORFNames=DDB_G0272564;
GN and
GN Name=psmD8-2; Synonyms=M4; ORFNames=DDB_G0273979;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 114-201.
RX PubMed=7465423; DOI=10.1093/nar/8.23.5599;
RA Kimmel A.R., Firtel R.A.;
RT "Intervening sequences in a Dictyostelium gene that encodes a low abundance
RT class mRNA.";
RL Nucleic Acids Res. 8:5599-5610(1980).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 114-125.
RX PubMed=2427932; DOI=10.1128/mcb.5.8.2123-2130.1985;
RA Kimmel A.R., Firtel R.A.;
RT "Sequence organization and developmental expression of an interspersed,
RT repetitive element and associated single-copy DNA sequences in
RT Dictyostelium discoideum.";
RL Mol. Cell. Biol. 5:2123-2130(1985).
CC -!- FUNCTION: Acts as a regulatory subunit of the 26S proteasome which is
CC involved in the ATP-dependent degradation of ubiquitinated proteins.
CC {ECO:0000250}.
CC -!- DEVELOPMENTAL STAGE: Potentially subject to developmental regulation.
CC -!- SIMILARITY: Belongs to the proteasome subunit S14 family.
CC {ECO:0000305}.
CC -!- CAUTION: The gene for this protein is duplicated in strains AX3 and
CC AX4. These strains contain a duplication of a segment of 750 kb of
CC chromosome 2 compared to the corresponding sequence in strain AX2.
CC {ECO:0000305}.
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DR EMBL; AAFI02000011; EAL70423.1; -; Genomic_DNA.
DR EMBL; AAFI02000009; EAL70919.1; -; Genomic_DNA.
DR EMBL; V00194; CAA23485.1; -; Genomic_DNA.
DR EMBL; M11429; AAA33223.1; -; Genomic_DNA.
DR PIR; A03384; QXDO.
DR RefSeq; XP_644348.1; XM_639256.1.
DR RefSeq; XP_645066.1; XM_639974.1.
DR AlphaFoldDB; P02889; -.
DR SMR; P02889; -.
DR STRING; 44689.DDB0185109; -.
DR PaxDb; P02889; -.
DR EnsemblProtists; EAL70423; EAL70423; DDB_G0273979.
DR EnsemblProtists; EAL70919; EAL70919; DDB_G0272564.
DR GeneID; 8618741; -.
DR GeneID; 8619235; -.
DR KEGG; ddi:DDB_G0272564; -.
DR KEGG; ddi:DDB_G0273979; -.
DR dictyBase; DDB_G0272564; psmD8-1.
DR dictyBase; DDB_G0273979; psmD8-2.
DR eggNOG; KOG3151; Eukaryota.
DR HOGENOM; CLU_046003_2_1_1; -.
DR InParanoid; P02889; -.
DR OMA; VYIRHPL; -.
DR PhylomeDB; P02889; -.
DR Reactome; R-DDI-1236978; Cross-presentation of soluble exogenous antigens (endosomes).
DR Reactome; R-DDI-174084; Autodegradation of Cdh1 by Cdh1:APC/C.
DR Reactome; R-DDI-174113; SCF-beta-TrCP mediated degradation of Emi1.
DR Reactome; R-DDI-174154; APC/C:Cdc20 mediated degradation of Securin.
DR Reactome; R-DDI-174178; APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
DR Reactome; R-DDI-187577; SCF(Skp2)-mediated degradation of p27/p21.
DR Reactome; R-DDI-2467813; Separation of Sister Chromatids.
DR Reactome; R-DDI-349425; Autodegradation of the E3 ubiquitin ligase COP1.
DR Reactome; R-DDI-450408; AUF1 (hnRNP D0) binds and destabilizes mRNA.
DR Reactome; R-DDI-4641258; Degradation of DVL.
DR Reactome; R-DDI-5632684; Hedgehog 'on' state.
DR Reactome; R-DDI-5658442; Regulation of RAS by GAPs.
DR Reactome; R-DDI-5687128; MAPK6/MAPK4 signaling.
DR Reactome; R-DDI-5689603; UCH proteinases.
DR Reactome; R-DDI-5689880; Ub-specific processing proteases.
DR Reactome; R-DDI-68949; Orc1 removal from chromatin.
DR Reactome; R-DDI-69017; CDK-mediated phosphorylation and removal of Cdc6.
DR Reactome; R-DDI-69601; Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
DR Reactome; R-DDI-75815; Ubiquitin-dependent degradation of Cyclin D.
DR Reactome; R-DDI-8854050; FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
DR Reactome; R-DDI-8948751; Regulation of PTEN stability and activity.
DR Reactome; R-DDI-8951664; Neddylation.
DR Reactome; R-DDI-9755511; KEAP1-NFE2L2 pathway.
DR Reactome; R-DDI-9762114; GSK3B and BTRC:CUL1-mediated-degradation of NFE2L2.
DR Reactome; R-DDI-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR PRO; PR:P02889; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0008541; C:proteasome regulatory particle, lid subcomplex; IBA:GO_Central.
DR GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR InterPro; IPR006746; 26S_Psome_Rpn12.
DR InterPro; IPR033464; CSN8_PSD8_EIF3K.
DR InterPro; IPR000717; PCI_dom.
DR PANTHER; PTHR12387; PTHR12387; 1.
DR Pfam; PF10075; CSN8_PSD8_EIF3K; 1.
DR PROSITE; PS50250; PCI; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Proteasome; Reference proteome.
FT CHAIN 1..263
FT /note="Probable 26S proteasome non-ATPase regulatory
FT subunit 8"
FT /id="PRO_0000173849"
FT DOMAIN 79..248
FT /note="PCI"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01185"
FT CONFLICT 185
FT /note="E -> Q (in Ref. 3; CAA23485)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 263 AA; 30364 MW; 002BE9B60EB21585 CRC64;
MDFSSIEQNL NNFKKLVAGN SDKAQITPVL VQLKLAATIH LEKPTSLSNV SDKVKKDLVL
AREILELISL YSIKIKDIDS FERTFNQLKT YYYDYKSIIA PSTLEYQIIG LNLMRLLAKH
KTSEFHSEIE LIEFNNLDNS FIKFPLLVEK SITEGSYNKI IQSRSGVPSE YYQVFLDILA
DSIKEDIANC SEKSFKTLSL KDAEKVLLFN DNNQFQQYIK ERNWKVQGDV IQFGNNDNQT
VEIPSLQLIH QTLHYAKELE RIV
