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PSMD9_YEAST
ID   PSMD9_YEAST             Reviewed;         220 AA.
AC   P40555; D6VVS3;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   03-AUG-2022, entry version 176.
DE   RecName: Full=Probable 26S proteasome regulatory subunit p27;
DE   AltName: Full=Proteasome non-ATPase subunit 2;
GN   Name=NAS2; OrderedLocusNames=YIL007C;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169870;
RA   Churcher C.M., Bowman S., Badcock K., Bankier A.T., Brown D.,
RA   Chillingworth T., Connor R., Devlin K., Gentles S., Hamlin N., Harris D.E.,
RA   Horsnell T., Hunt S., Jagels K., Jones M., Lye G., Moule S., Odell C.,
RA   Pearson D., Rajandream M.A., Rice P., Rowley N., Skelton J., Smith V.,
RA   Walsh S.V., Whitehead S., Barrell B.G.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome IX.";
RL   Nature 387:84-87(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [4]
RP   CHARACTERIZATION.
RX   PubMed=9653651; DOI=10.1006/geno.1998.5301;
RA   Watanabe T.K., Saito A., Suzuki M., Fujiwara T., Takahashi E.,
RA   Slaughter C.A., DeMartino G.N., Hendil K.B., Chung C.H., Tanahashi N.,
RA   Tanaka K.;
RT   "cDNA cloning and characterization of a human proteasomal modulator
RT   subunit, p27 (PSMD9).";
RL   Genomics 50:241-250(1998).
RN   [5]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [6]
RP   FUNCTION AS PROTEASOME CHAPERONE.
RX   PubMed=19446322; DOI=10.1016/j.cell.2009.04.061;
RA   Funakoshi M., Tomko R.J. Jr., Kobayashi H., Hochstrasser M.;
RT   "Multiple assembly chaperones govern biogenesis of the proteasome
RT   regulatory particle base.";
RL   Cell 137:887-899(2009).
RN   [7]
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA   Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA   Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA   Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT   "N-terminal acetylome analyses and functional insights of the N-terminal
RT   acetyltransferase NatB.";
RL   Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC   -!- FUNCTION: Acts as a chaperone during the assembly of the 26S
CC       proteasome, specifically of the base subcomplex of the 19S regulatory
CC       complex (RC). During the base subcomplex assembly is part of a
CC       NAS2:RPT4:RPT5 module; NAS2 is released during the further base
CC       assembly process. {ECO:0000269|PubMed:19446322}.
CC   -!- SUBUNIT: Part of a transient complex containing NAS2, RPT4 and RPT5
CC       formed during the assembly of the 26S proteasome.
CC   -!- INTERACTION:
CC       P40555; P38872: PFS1; NbExp=3; IntAct=EBI-14024, EBI-24859;
CC       P40555; P33297: RPT5; NbExp=8; IntAct=EBI-14024, EBI-13920;
CC   -!- MISCELLANEOUS: Present with 846 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the proteasome subunit p27 family.
CC       {ECO:0000305}.
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DR   EMBL; Z38113; CAA86244.1; -; Genomic_DNA.
DR   EMBL; AY558294; AAS56620.1; -; Genomic_DNA.
DR   EMBL; BK006942; DAA08539.1; -; Genomic_DNA.
DR   PIR; S48450; S48450.
DR   RefSeq; NP_012259.3; NM_001179357.3.
DR   PDB; 3WHJ; X-ray; 1.65 A; A=1-120.
DR   PDB; 3WHL; X-ray; 4.00 A; B/D/F/H=1-120.
DR   PDB; 4O06; X-ray; 1.15 A; A=126-220.
DR   PDBsum; 3WHJ; -.
DR   PDBsum; 3WHL; -.
DR   PDBsum; 4O06; -.
DR   AlphaFoldDB; P40555; -.
DR   SMR; P40555; -.
DR   BioGRID; 34985; 131.
DR   DIP; DIP-1893N; -.
DR   IntAct; P40555; 19.
DR   MINT; P40555; -.
DR   STRING; 4932.YIL007C; -.
DR   MaxQB; P40555; -.
DR   PaxDb; P40555; -.
DR   PRIDE; P40555; -.
DR   EnsemblFungi; YIL007C_mRNA; YIL007C; YIL007C.
DR   GeneID; 854810; -.
DR   KEGG; sce:YIL007C; -.
DR   SGD; S000001269; NAS2.
DR   VEuPathDB; FungiDB:YIL007C; -.
DR   eggNOG; KOG3129; Eukaryota.
DR   GeneTree; ENSGT00390000004147; -.
DR   HOGENOM; CLU_073146_0_0_1; -.
DR   InParanoid; P40555; -.
DR   OMA; GCNIVPL; -.
DR   BioCyc; YEAST:G3O-31286-MON; -.
DR   Reactome; R-SCE-1236978; Cross-presentation of soluble exogenous antigens (endosomes).
DR   Reactome; R-SCE-382556; ABC-family proteins mediated transport.
DR   Reactome; R-SCE-5668541; TNFR2 non-canonical NF-kB pathway.
DR   Reactome; R-SCE-5687128; MAPK6/MAPK4 signaling.
DR   Reactome; R-SCE-5689880; Ub-specific processing proteases.
DR   Reactome; R-SCE-68949; Orc1 removal from chromatin.
DR   Reactome; R-SCE-69017; CDK-mediated phosphorylation and removal of Cdc6.
DR   Reactome; R-SCE-69601; Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
DR   Reactome; R-SCE-75815; Ubiquitin-dependent degradation of Cyclin D.
DR   Reactome; R-SCE-8854050; FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
DR   Reactome; R-SCE-8948751; Regulation of PTEN stability and activity.
DR   Reactome; R-SCE-8951664; Neddylation.
DR   Reactome; R-SCE-9755511; KEAP1-NFE2L2 pathway.
DR   Reactome; R-SCE-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR   PRO; PR:P40555; -.
DR   Proteomes; UP000002311; Chromosome IX.
DR   RNAct; P40555; protein.
DR   GO; GO:0005737; C:cytoplasm; IDA:SGD.
DR   GO; GO:0005829; C:cytosol; IDA:SGD.
DR   GO; GO:0005634; C:nucleus; IDA:SGD.
DR   GO; GO:0070682; P:proteasome regulatory particle assembly; IDA:SGD.
DR   Gene3D; 2.30.42.10; -; 1.
DR   InterPro; IPR040815; Nas2_N.
DR   InterPro; IPR001478; PDZ.
DR   InterPro; IPR036034; PDZ_sf.
DR   InterPro; IPR035269; PSMD9.
DR   PANTHER; PTHR12651; PTHR12651; 1.
DR   Pfam; PF18265; Nas2_N; 1.
DR   Pfam; PF00595; PDZ; 1.
DR   SMART; SM00228; PDZ; 1.
DR   SUPFAM; SSF50156; SSF50156; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Chaperone; Reference proteome.
FT   CHAIN           1..220
FT                   /note="Probable 26S proteasome regulatory subunit p27"
FT                   /id="PRO_0000173856"
FT   DOMAIN          119..196
FT                   /note="PDZ"
FT   HELIX           2..11
FT                   /evidence="ECO:0007829|PDB:3WHJ"
FT   HELIX           17..24
FT                   /evidence="ECO:0007829|PDB:3WHJ"
FT   TURN            25..28
FT                   /evidence="ECO:0007829|PDB:3WHJ"
FT   HELIX           31..54
FT                   /evidence="ECO:0007829|PDB:3WHJ"
FT   HELIX           73..110
FT                   /evidence="ECO:0007829|PDB:3WHJ"
FT   STRAND          135..140
FT                   /evidence="ECO:0007829|PDB:4O06"
FT   HELIX           145..148
FT                   /evidence="ECO:0007829|PDB:4O06"
FT   STRAND          156..160
FT                   /evidence="ECO:0007829|PDB:4O06"
FT   TURN            165..167
FT                   /evidence="ECO:0007829|PDB:4O06"
FT   TURN            169..171
FT                   /evidence="ECO:0007829|PDB:4O06"
FT   HELIX           172..180
FT                   /evidence="ECO:0007829|PDB:4O06"
FT   TURN            181..183
FT                   /evidence="ECO:0007829|PDB:4O06"
FT   STRAND          186..192
FT                   /evidence="ECO:0007829|PDB:4O06"
FT   STRAND          195..201
FT                   /evidence="ECO:0007829|PDB:4O06"
FT   STRAND          207..213
FT                   /evidence="ECO:0007829|PDB:4O06"
FT   STRAND          215..219
FT                   /evidence="ECO:0007829|PDB:4O06"
SQ   SEQUENCE   220 AA;  24846 MW;  1BA966C5B7B2D02B CRC64;
     MEEEELSKLL ANVKIDPSLT SRISQIDSFK LSELMVLKTD IETQLEAYFS VLEQQGIGMD
     SALVTPDGYP RSDVDVLQVT MIRKNVNMLK NDLNHLLQRS HVLLNQHFDN MNVKSNQDAR
     RNNDDQAIQY TIPFAFISEV VPGSPSDKAD IKVDDKLISI GNVHAANHSK LQNIQMVVMK
     NEDRPLPVLL LREGQILKTS LTPSRNWNGR GLLGCRIQEL
 
 
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