ID   PSME4_ARATH             Reviewed;        1811 AA.
AC   F4JC97; Q8RXU1; Q9LTT5;
DT   24-JUL-2013, integrated into UniProtKB/Swiss-Prot.
DT   24-JUL-2013, sequence version 2.
DT   25-MAY-2022, entry version 69.
DE   RecName: Full=Proteasome activator subunit 4;
DE   AltName: Full=Proteasome activator PA200;
GN   Name=PA200; OrderedLocusNames=At3g13330; ORFNames=MDC11.16;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA   Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT   features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT   clones.";
RL   DNA Res. 7:131-135(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1632-1811.
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   IDENTIFICATION IN THE PROTEASOME, DISRUPTION PHENOTYPE, AND INDUCTION.
RX   PubMed=20516081; DOI=10.1074/jbc.m110.136622;
RA   Book A.J., Gladman N.P., Lee S.S., Scalf M., Smith L.M., Vierstra R.D.;
RT   "Affinity purification of the Arabidopsis 26 S proteasome reveals a diverse
RT   array of plant proteolytic complexes.";
RL   J. Biol. Chem. 285:25554-25569(2010).
CC   -!- FUNCTION: Associated component of the proteasome that specifically
CC       recognizes acetylated histones and promotes ATP- and ubiquitin-
CC       independent degradation of core histones during DNA damage response.
CC       Recognizes and binds acetylated histones via its bromodomain-like
CC       (BRDL) region and activates the proteasome by opening the gated channel
CC       for substrate entry. Binds to the core proteasome via its C-terminus,
CC       which occupies the same binding sites as the proteasomal ATPases,
CC       opening the closed structure of the proteasome via an active gating
CC       mechanism. involved in DNA damage response: binds to acetylated
CC       histones and promotes degradation of histones (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with the 26S proteasomes.
CC       {ECO:0000269|PubMed:20516081}.
CC   -!- INDUCTION: Upon MG132 treatment. {ECO:0000269|PubMed:20516081}.
CC   -!- DOMAIN: The bromodomain-like (BRDL) region specifically recognizes and
CC       binds acetylated histones. {ECO:0000250}.
CC   -!- DISRUPTION PHENOTYPE: No visible phenotype.
CC       {ECO:0000269|PubMed:20516081}.
CC   -!- SIMILARITY: Belongs to the BLM10 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB02802.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AB024034; BAB02802.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002686; AEE75334.2; -; Genomic_DNA.
DR   EMBL; AY080673; AAL86349.1; -; mRNA.
DR   RefSeq; NP_001319538.1; NM_001338043.1.
DR   AlphaFoldDB; F4JC97; -.
DR   SMR; F4JC97; -.
DR   BioGRID; 5867; 1.
DR   STRING; 3702.AT3G13330.1; -.
DR   iPTMnet; F4JC97; -.
DR   PaxDb; F4JC97; -.
DR   PRIDE; F4JC97; -.
DR   ProteomicsDB; 248902; -.
DR   EnsemblPlants; AT3G13330.1; AT3G13330.1; AT3G13330.
DR   GeneID; 820533; -.
DR   Gramene; AT3G13330.1; AT3G13330.1; AT3G13330.
DR   KEGG; ath:AT3G13330; -.
DR   Araport; AT3G13330; -.
DR   TAIR; locus:2088140; AT3G13330.
DR   eggNOG; KOG1851; Eukaryota.
DR   HOGENOM; CLU_000772_3_0_1; -.
DR   InParanoid; F4JC97; -.
DR   OMA; GKDWSDE; -.
DR   OrthoDB; 83617at2759; -.
DR   PRO; PR:F4JC97; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; F4JC97; baseline and differential.
DR   Genevisible; F4JC97; AT.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0000502; C:proteasome complex; IEA:UniProtKB-KW.
DR   GO; GO:0070577; F:lysine-acetylated histone binding; IBA:GO_Central.
DR   GO; GO:0016504; F:peptidase activator activity; IBA:GO_Central.
DR   GO; GO:0070628; F:proteasome binding; IBA:GO_Central.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   GO; GO:0010499; P:proteasomal ubiquitin-independent protein catabolic process; IBA:GO_Central.
DR   Gene3D; 1.25.10.10; -; 1.
DR   InterPro; IPR011989; ARM-like.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR032430; Blm10_mid.
DR   InterPro; IPR035309; PSME4.
DR   InterPro; IPR021843; PSME4_C.
DR   PANTHER; PTHR32170; PTHR32170; 1.
DR   Pfam; PF16507; BLM10_mid; 2.
DR   Pfam; PF11919; DUF3437; 1.
DR   SUPFAM; SSF48371; SSF48371; 2.
PE   2: Evidence at transcript level;
KW   DNA damage; DNA repair; Proteasome; Reference proteome; Repeat.
FT   CHAIN           1..1811
FT                   /note="Proteasome activator subunit 4"
FT                   /id="PRO_0000423302"
FT   REPEAT          563..599
FT                   /note="HEAT 1"
FT   REPEAT          1156..1193
FT                   /note="HEAT 2"
FT   REPEAT          1442..1479
FT                   /note="HEAT 3"
FT   REPEAT          1613..1650
FT                   /note="HEAT 4"
FT   REPEAT          1655..1692
FT                   /note="HEAT 5"
FT   REPEAT          1746..1783
FT                   /note="HEAT 6"
FT   REGION          1626..1711
FT                   /note="Bromodomain-like (BRDL)"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   1811 AA;  202946 MW;  0CB33FA589D3AC9D CRC64;
     MHLYNEWLPP PVAEETKKEK ESFARVVRCV KELHRPDDPE SVYATLKWIS VIELFVRAKS
     ELSVEDVSEL VEIGLQIFHS SENKLYAQVR WGNVLVRLIN KFRKKLSLKV QWRPLYDTLI
     HAHFSRSPGP EGWRLRQRHF MAVTSLIRSC RRFFPQGAAS EIWSEFMSLL ENPWHNSSFE
     GSGFVRLFLP TNPENQDFFS EKWIKNVLEL WDSIPNCQFW NSQWTSVLAR VIKNCSFIDW
     ESYLPMLFSR FLNMFEVPVA NGSGSYPFSV DVPRNTRFLF SNRTTTPSKS IAQSIVYFLK
     PGSSAHEQLE KLVNLLEQYY HPSNGGRWTY SLERFLLHLV IAFQKRLQRE QQDPDSLPAT
     CLGKPERVAF VGVVLKLIDR GQYSKNEHLS ETVAAATSML SYVEPSLVLP FVASRFHLAL
     ETTTATHQLK TAMMSVAFAG RSILQSSMST AKSQDLGGDV DDRMFLDLIG ISLSNALLGM
     DANDPPKTLA TMQLIGSIFS NMAVLDDSSD DLSFMTMASF SEWLDEFLCR LIALLQHLEP
     NSVINEGLSS SATSGTFLVE DGPYYYCMLE ILLGRLSGSL YNQALKKISK FVQTNILPGA
     IAEVGLLCCA CVHSTPEEAV AQIVEPMLLA VISSLKEIPV NGYGGKGSAE TLVSNKQDKQ
     TLSPALEAAI DYQLKVLSVA ITYGGSSLLP YKGLLIEAIS SAFNSSSWKV NGAGDHLLRS
     LLGSLILYYP IDQYKCLSRH PAAPALEEWI STKASSKDEQ VAHSRWHVPT QEETQFANEL
     LDLHLQSALD DLLSICQSNI HSDAGDEKTH LKVTLLRIDS TLQGVLSCLP DFRPSPRHDM
     VEDLQFFIAG ASGSCVGSAE IREKTAITIH AACKYLLEKK SDDSILLILI IRIMDALGNY
     GSLEYDEWSN HRQAWKLESA AIVEPPANFI TEFNSKGKRR PRWALIDKAY MHNTWRSSQS
     SYHLFRTDGN FSPPEPLTFL VDDLLTLCLH NYETVRVLAG KSLIKLLKRW PQLLSKCVLS
     LTENLRKPDV QEYVVLGSCA ILSSHSVLKH LTTDPKSFSS FLLGILSSSH HESMKSQKAI
     IELFVKYNIH FAGLSRNILR SLESHVEGST SGDLVSQIGS MSFDSSSLHW RYNLMANRVL
     LLLVMSSRID PSFSLKILDE TAGHFLKNLK SQLPQTRILA ISALNILLKE SPHKMQGKDQ
     PSVSSQETEN ANSSLDLALS QIFREEGFFK ETFESLSHIH ITDTDSSSRG NHGSSSFQSM
     ADKSITRFYF EFSASWPRTP SWISLLGSDI FYPSFARIFK RLAQECGVPV LLALKSPLEE
     FCNAKERPKQ CVAAEALAGV LHSDVNGLFN EWDSWIMVQL QNVILGQSVE SIPEWAACIR
     YAVTGKGKQG TKIPVMRQQI LDCIVAPLPP TATTTVVAKR YAFLSAALIE LSPPKMPVTE
     VKLHIVLLDE LICNMSHSSA QIREAIGVIL SVLCSNIRLR MSYQQEYPTE EGKTDVDSQL
     KEENWFKLIS AKASEAVKNI QQASISDSLD TSTDVDMGNA QSNGDSLDDV KWMETLFHFI
     ISSFKSGRAS YLLDVIAGFL YPVMSLQETS HKDLSILAKA AFELLKWRVF PESHLQKVIG
     VILSSADDSN WRIRSSTLTY LRTFMYRHTF ILTHEDKQKI WKTVEKLLVD SQVEVREHAA
     AVLAGLMKGG DEDFAADFRD RSYAEANSIQ KRRNRRKSSS TQSIAGVHGA VLGLVASVLS
     VPYDMPSWLP EHVTLLARFA GEPTPIKSTV TKAVAEFRRT HADTWNIQKD SFTEDQLEIL
     ADTSSSSSYF A