PSMG3_HUMAN
ID PSMG3_HUMAN Reviewed; 122 AA.
AC Q9BT73; A4D216; A8MPW2;
DT 09-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Proteasome assembly chaperone 3;
DE Short=PAC-3;
DE Short=hPAC3;
GN Name=PSMG3; Synonyms=C7orf48, PAC3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12853948; DOI=10.1038/nature01782;
RA Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K.,
RA Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A.,
RA Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H.,
RA Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A.,
RA Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P.,
RA Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M.,
RA Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S.,
RA Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R.,
RA Strowmatt C., Latreille P., Miller N., Johnson D., Murray J.,
RA Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W.,
RA Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A.,
RA Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E.,
RA Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A.,
RA Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A.,
RA Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R.,
RA McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H.,
RA Wilson R.K.;
RT "The DNA sequence of human chromosome 7.";
RL Nature 424:157-164(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12690205; DOI=10.1126/science.1083423;
RA Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K.,
RA Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R.,
RA Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A.,
RA Kanematsu E., Gentles S., Christopoulos C.C., Choufani S., Kwasnicka D.,
RA Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S.,
RA Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R.,
RA Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N.,
RA Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E.,
RA Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R.,
RA Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T.,
RA Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W.,
RA Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A.,
RA Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X.,
RA Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E.,
RA Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H.,
RA Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J.,
RA Adams M.D., Tsui L.-C.;
RT "Human chromosome 7: DNA sequence and biology.";
RL Science 300:767-772(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Adrenal cortex, and Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION, FUNCTION, AND INTERACTION WITH THE PROTEASOME.
RX PubMed=17189198; DOI=10.1016/j.molcel.2006.11.015;
RA Hirano Y., Hayashi H., Iemura S., Hendil K.B., Niwa S., Kishimoto T.,
RA Kasahara M., Natsume T., Tanaka K., Murata S.;
RT "Cooperation of multiple chaperones required for the assembly of mammalian
RT 20S proteasomes.";
RL Mol. Cell 24:977-984(2006).
RN [6]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19413330; DOI=10.1021/ac9004309;
RA Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.;
RT "Lys-N and trypsin cover complementary parts of the phosphoproteome in a
RT refined SCX-based approach.";
RL Anal. Chem. 81:4493-4501(2009).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [8]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT MET-1, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22223895; DOI=10.1074/mcp.m111.015131;
RA Bienvenut W.V., Sumpton D., Martinez A., Lilla S., Espagne C., Meinnel T.,
RA Giglione C.;
RT "Comparative large-scale characterisation of plant vs. mammal proteins
RT reveals similar and idiosyncratic N-alpha acetylation features.";
RL Mol. Cell. Proteomics 11:M111.015131-M111.015131(2012).
RN [9]
RP X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS), AND SUBUNIT.
RX PubMed=18278057; DOI=10.1038/nsmb.1386;
RA Yashiroda H., Mizushima T., Okamoto K., Kameyama T., Hayashi H.,
RA Kishimoto T., Niwa S., Kasahara M., Kurimoto E., Sakata E., Takagi K.,
RA Suzuki A., Hirano Y., Murata S., Kato K., Yamane T., Tanaka K.;
RT "Crystal structure of a chaperone complex that contributes to the assembly
RT of yeast 20S proteasomes.";
RL Nat. Struct. Mol. Biol. 15:228-236(2008).
CC -!- FUNCTION: Chaperone protein which promotes assembly of the 20S
CC proteasome. May cooperate with PSMG1-PSMG2 heterodimers to orchestrate
CC the correct assembly of proteasomes. {ECO:0000269|PubMed:17189198}.
CC -!- SUBUNIT: Homodimer. Interacts with PSMG4. Interacts directly with alpha
CC and beta subunits of the 20S proteasome but dissociates before the
CC formation of half-proteasomes, probably upon recruitment of POMP.
CC {ECO:0000269|PubMed:17189198, ECO:0000269|PubMed:18278057}.
CC -!- SIMILARITY: Belongs to the PSMG3 family. {ECO:0000305}.
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DR EMBL; AC093734; AAP21867.1; -; Genomic_DNA.
DR EMBL; CH236953; EAL23945.1; -; Genomic_DNA.
DR EMBL; CH471144; EAW87212.1; -; Genomic_DNA.
DR EMBL; BC004308; AAH04308.1; -; mRNA.
DR EMBL; BC027171; AAH27171.1; -; mRNA.
DR EMBL; BR000337; FAA00325.1; -; mRNA.
DR CCDS; CCDS5327.1; -.
DR RefSeq; NP_001127812.1; NM_001134340.1.
DR RefSeq; NP_115678.1; NM_032302.3.
DR PDB; 2Z5E; X-ray; 2.00 A; A/B=1-122.
DR PDB; 6JPT; X-ray; 0.96 A; A=1-122.
DR PDBsum; 2Z5E; -.
DR PDBsum; 6JPT; -.
DR AlphaFoldDB; Q9BT73; -.
DR SMR; Q9BT73; -.
DR BioGRID; 123989; 108.
DR CORUM; Q9BT73; -.
DR IntAct; Q9BT73; 34.
DR MINT; Q9BT73; -.
DR STRING; 9606.ENSP00000288607; -.
DR BindingDB; Q9BT73; -.
DR ChEMBL; CHEMBL1075137; -.
DR GlyGen; Q9BT73; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q9BT73; -.
DR PhosphoSitePlus; Q9BT73; -.
DR BioMuta; PSMG3; -.
DR DMDM; 74733099; -.
DR EPD; Q9BT73; -.
DR jPOST; Q9BT73; -.
DR MassIVE; Q9BT73; -.
DR MaxQB; Q9BT73; -.
DR PaxDb; Q9BT73; -.
DR PeptideAtlas; Q9BT73; -.
DR PRIDE; Q9BT73; -.
DR ProteomicsDB; 78955; -.
DR TopDownProteomics; Q9BT73; -.
DR Antibodypedia; 10998; 69 antibodies from 18 providers.
DR DNASU; 84262; -.
DR Ensembl; ENST00000252329.3; ENSP00000252329.3; ENSG00000157778.9.
DR Ensembl; ENST00000288607.3; ENSP00000288607.2; ENSG00000157778.9.
DR Ensembl; ENST00000404674.7; ENSP00000384799.3; ENSG00000157778.9.
DR GeneID; 84262; -.
DR KEGG; hsa:84262; -.
DR MANE-Select; ENST00000288607.3; ENSP00000288607.2; NM_032302.4; NP_115678.1.
DR UCSC; uc003skx.3; human.
DR CTD; 84262; -.
DR DisGeNET; 84262; -.
DR GeneCards; PSMG3; -.
DR HGNC; HGNC:22420; PSMG3.
DR HPA; ENSG00000157778; Low tissue specificity.
DR MIM; 617528; gene.
DR neXtProt; NX_Q9BT73; -.
DR OpenTargets; ENSG00000157778; -.
DR PharmGKB; PA162400247; -.
DR VEuPathDB; HostDB:ENSG00000157778; -.
DR eggNOG; KOG4828; Eukaryota.
DR GeneTree; ENSGT00390000000324; -.
DR HOGENOM; CLU_133503_1_0_1; -.
DR InParanoid; Q9BT73; -.
DR OMA; IRTCQVW; -.
DR OrthoDB; 1469039at2759; -.
DR PhylomeDB; Q9BT73; -.
DR TreeFam; TF300294; -.
DR PathwayCommons; Q9BT73; -.
DR SignaLink; Q9BT73; -.
DR BioGRID-ORCS; 84262; 733 hits in 1079 CRISPR screens.
DR ChiTaRS; PSMG3; human.
DR EvolutionaryTrace; Q9BT73; -.
DR GenomeRNAi; 84262; -.
DR Pharos; Q9BT73; Tchem.
DR PRO; PR:Q9BT73; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; Q9BT73; protein.
DR Bgee; ENSG00000157778; Expressed in mucosa of transverse colon and 177 other tissues.
DR ExpressionAtlas; Q9BT73; baseline and differential.
DR Genevisible; Q9BT73; HS.
DR GO; GO:0032991; C:protein-containing complex; ISS:UniProtKB.
DR GO; GO:0060090; F:molecular adaptor activity; IMP:UniProtKB.
DR GO; GO:0044877; F:protein-containing complex binding; IDA:UniProtKB.
DR GO; GO:0051131; P:chaperone-mediated protein complex assembly; IMP:UniProtKB.
DR GO; GO:0043248; P:proteasome assembly; IEA:InterPro.
DR InterPro; IPR018788; Proteasome_assmbl_chp_3.
DR PANTHER; PTHR31051; PTHR31051; 1.
DR Pfam; PF10178; PAC3; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Acetylation; Chaperone; Reference proteome.
FT CHAIN 1..122
FT /note="Proteasome assembly chaperone 3"
FT /id="PRO_0000271358"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0007744|PubMed:19413330,
FT ECO:0007744|PubMed:22223895"
FT STRAND 6..16
FT /evidence="ECO:0007829|PDB:6JPT"
FT STRAND 19..27
FT /evidence="ECO:0007829|PDB:6JPT"
FT STRAND 29..38
FT /evidence="ECO:0007829|PDB:6JPT"
FT STRAND 44..51
FT /evidence="ECO:0007829|PDB:6JPT"
FT STRAND 56..59
FT /evidence="ECO:0007829|PDB:2Z5E"
FT STRAND 61..69
FT /evidence="ECO:0007829|PDB:6JPT"
FT HELIX 73..89
FT /evidence="ECO:0007829|PDB:6JPT"
FT STRAND 94..100
FT /evidence="ECO:0007829|PDB:6JPT"
FT HELIX 106..118
FT /evidence="ECO:0007829|PDB:6JPT"
SQ SEQUENCE 122 AA; 13104 MW; FA3D42A2CF4B88B7 CRC64;
MEDTPLVISK QKTEVVCGVP TQVVCTAFSS HILVVVTQFG KMGTLVSLEP SSVASDVSKP
VLTTKVLLGQ DEPLIHVFAK NLVAFVSQEA GNRAVLLAVA VKDKSMEGLK ALREVIRVCQ
VW
