PSMG4_HUMAN
ID PSMG4_HUMAN Reviewed; 123 AA.
AC Q5JS54; C9J2F8; F8WBZ2; Q5JS53; Q5JS56;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 10-JUN-2008, sequence version 2.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Proteasome assembly chaperone 4;
DE Short=PAC-4;
DE Short=hPAC4;
GN Name=PSMG4; Synonyms=C6orf86, PAC4;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=14574404; DOI=10.1038/nature02055;
RA Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA Rogers J., Beck S.;
RT "The DNA sequence and analysis of human chromosome 6.";
RL Nature 425:805-811(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Embryonic stem cell;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION.
RX PubMed=17707236; DOI=10.1016/j.molcel.2007.06.025;
RA Le Tallec B., Barrault M.-B., Courbeyrette R., Guerois R.,
RA Marsolier-Kergoat M.-C., Peyroche A.;
RT "20S proteasome assembly is orchestrated by two distinct pairs of
RT chaperones in yeast and in mammals.";
RL Mol. Cell 27:660-674(2007).
RN [5]
RP VARIANT ARG-99 (ISOFORM 2).
RX PubMed=28501589; DOI=10.1016/j.clim.2017.05.009;
RA Sadovnick A.D., Traboulsee A.L., Zhao Y., Bernales C.Q., Encarnacion M.,
RA Ross J.P., Yee I.M., Criscuoli M.G., Vilarino-Gueell C.;
RT "Genetic modifiers of multiple sclerosis progression, severity and onset.";
RL Clin. Immunol. 180:100-105(2017).
CC -!- FUNCTION: Chaperone protein which promotes assembly of the 20S
CC proteasome. {ECO:0000250|UniProtKB:P0C7N9}.
CC -!- SUBUNIT: Interacts with PSMG3. Associates with alpha subunits of the
CC 20S proteasome. {ECO:0000250|UniProtKB:P0C7N9}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q5JS54-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5JS54-2; Sequence=VSP_046635;
CC Name=3;
CC IsoId=Q5JS54-3; Sequence=VSP_046636;
CC -!- SIMILARITY: Belongs to the PSMG4 family. {ECO:0000305}.
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DR EMBL; AL445309; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471087; EAW55130.1; -; Genomic_DNA.
DR EMBL; CD173424; -; NOT_ANNOTATED_CDS; mRNA.
DR CCDS; CCDS47360.1; -. [Q5JS54-2]
DR CCDS; CCDS47361.1; -. [Q5JS54-1]
DR CCDS; CCDS47362.1; -. [Q5JS54-3]
DR RefSeq; NP_001122063.1; NM_001128591.1. [Q5JS54-1]
DR RefSeq; NP_001122064.1; NM_001128592.1. [Q5JS54-2]
DR RefSeq; NP_001129222.1; NM_001135750.1. [Q5JS54-3]
DR PDB; 5WTQ; X-ray; 1.90 A; A/B/C/D=1-123.
DR PDBsum; 5WTQ; -.
DR AlphaFoldDB; Q5JS54; -.
DR SMR; Q5JS54; -.
DR BioGRID; 133110; 62.
DR CORUM; Q5JS54; -.
DR IntAct; Q5JS54; 16.
DR GlyGen; Q5JS54; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q5JS54; -.
DR PhosphoSitePlus; Q5JS54; -.
DR BioMuta; PSMG4; -.
DR DMDM; 190360085; -.
DR EPD; Q5JS54; -.
DR jPOST; Q5JS54; -.
DR MassIVE; Q5JS54; -.
DR MaxQB; Q5JS54; -.
DR PeptideAtlas; Q5JS54; -.
DR PRIDE; Q5JS54; -.
DR ProteomicsDB; 30974; -.
DR ProteomicsDB; 63135; -. [Q5JS54-1]
DR ProteomicsDB; 8201; -.
DR TopDownProteomics; Q5JS54-1; -. [Q5JS54-1]
DR Antibodypedia; 78115; 4 antibodies from 4 providers.
DR DNASU; 389362; -.
DR Ensembl; ENST00000419065.6; ENSP00000392353.2; ENSG00000180822.12. [Q5JS54-2]
DR Ensembl; ENST00000438998.7; ENSP00000413353.3; ENSG00000180822.12. [Q5JS54-1]
DR Ensembl; ENST00000473000.2; ENSP00000418241.2; ENSG00000180822.12. [Q5JS54-3]
DR GeneID; 389362; -.
DR KEGG; hsa:389362; -.
DR MANE-Select; ENST00000438998.7; ENSP00000413353.3; NM_001128591.2; NP_001122063.1.
DR UCSC; uc003mvl.2; human. [Q5JS54-1]
DR CTD; 389362; -.
DR DisGeNET; 389362; -.
DR GeneCards; PSMG4; -.
DR HGNC; HGNC:21108; PSMG4.
DR HPA; ENSG00000180822; Low tissue specificity.
DR MIM; 617550; gene.
DR neXtProt; NX_Q5JS54; -.
DR OpenTargets; ENSG00000180822; -.
DR PharmGKB; PA162400256; -.
DR VEuPathDB; HostDB:ENSG00000180822; -.
DR GeneTree; ENSGT00390000011804; -.
DR HOGENOM; CLU_138031_0_0_1; -.
DR InParanoid; Q5JS54; -.
DR OMA; CSPRDSI; -.
DR OrthoDB; 1468993at2759; -.
DR PhylomeDB; Q5JS54; -.
DR TreeFam; TF353302; -.
DR PathwayCommons; Q5JS54; -.
DR SignaLink; Q5JS54; -.
DR BioGRID-ORCS; 389362; 733 hits in 1090 CRISPR screens.
DR ChiTaRS; PSMG4; human.
DR GenomeRNAi; 389362; -.
DR Pharos; Q5JS54; Tdark.
DR PRO; PR:Q5JS54; -.
DR Proteomes; UP000005640; Chromosome 6.
DR RNAct; Q5JS54; protein.
DR Bgee; ENSG00000180822; Expressed in oocyte and 193 other tissues.
DR ExpressionAtlas; Q5JS54; baseline and differential.
DR Genevisible; Q5JS54; HS.
DR GO; GO:0032991; C:protein-containing complex; ISS:UniProtKB.
DR GO; GO:0044877; F:protein-containing complex binding; ISS:UniProtKB.
DR GO; GO:0043248; P:proteasome assembly; IEA:InterPro.
DR InterPro; IPR032157; PAC4.
DR PANTHER; PTHR33559; PTHR33559; 1.
DR Pfam; PF16093; PAC4; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Chaperone; Reference proteome.
FT CHAIN 1..123
FT /note="Proteasome assembly chaperone 4"
FT /id="PRO_0000341397"
FT VAR_SEQ 83
FT /note="L -> LGLGGKTGLACECGVEWGLSKGHEAECSTLPTPQHTSCGP (in
FT isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_046635"
FT VAR_SEQ 84..123
FT /note="ARKTNKQVFVSYNLQNTDSNFALLVENRIKEEMEAFPEKF -> GMYPQLAL
FT HGQPGGAHSLMEP (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_046636"
FT CONFLICT 47
FT /note="L -> Q (in Ref. 3; CD173424)"
FT /evidence="ECO:0000305"
FT STRAND 13..21
FT /evidence="ECO:0007829|PDB:5WTQ"
FT STRAND 24..32
FT /evidence="ECO:0007829|PDB:5WTQ"
FT STRAND 34..42
FT /evidence="ECO:0007829|PDB:5WTQ"
FT STRAND 50..54
FT /evidence="ECO:0007829|PDB:5WTQ"
FT STRAND 57..61
FT /evidence="ECO:0007829|PDB:5WTQ"
FT STRAND 63..66
FT /evidence="ECO:0007829|PDB:5WTQ"
FT HELIX 75..87
FT /evidence="ECO:0007829|PDB:5WTQ"
FT STRAND 88..95
FT /evidence="ECO:0007829|PDB:5WTQ"
FT HELIX 102..118
FT /evidence="ECO:0007829|PDB:5WTQ"
FT HELIX 120..122
FT /evidence="ECO:0007829|PDB:5WTQ"
FT VARIANT Q5JS54-2:99
FT /note="W -> R (in dbSNP:rs4959786)"
FT /evidence="ECO:0000269|PubMed:28501589"
FT /id="VAR_084585"
SQ SEQUENCE 123 AA; 13775 MW; 44F98C0CC00B937F CRC64;
MEGLVVAAGG DVSLHNFSAR LWEQLVHFHV MRLTDSLFLW VGATPHLRNL AVAMCSRYDS
IPVSTSLLGD TSDTTSTGLA QRLARKTNKQ VFVSYNLQNT DSNFALLVEN RIKEEMEAFP
EKF
