PSNB_ARATH
ID PSNB_ARATH Reviewed; 397 AA.
AC Q9SIK7; Q0IGK6;
DT 26-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Presenilin-like protein At2g29900;
GN OrderedLocusNames=At2g29900; ORFNames=F6K5.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Quinitio C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (SEP-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Probable subunit of the gamma-secretase complex, an
CC endoprotease complex that catalyzes the intramembrane cleavage of
CC integral membrane proteins such as Notch receptors. {ECO:0000250}.
CC -!- SUBUNIT: Homodimer. Probable component of the gamma-secretase complex,
CC a complex composed of a presenilin homodimer, nicastrin, APH1 and PEN2
CC (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}. Golgi apparatus membrane
CC {ECO:0000250}; Multi-pass membrane protein {ECO:0000250}.
CC -!- DOMAIN: The PAL motif is required for normal active site conformation.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the peptidase A22A family. {ECO:0000305}.
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DR EMBL; AC007113; AAD23630.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC08318.1; -; Genomic_DNA.
DR EMBL; BT028914; ABI49461.1; -; mRNA.
DR PIR; A84702; A84702.
DR RefSeq; NP_180551.1; NM_128544.4.
DR AlphaFoldDB; Q9SIK7; -.
DR SMR; Q9SIK7; -.
DR BioGRID; 2890; 2.
DR STRING; 3702.AT2G29900.1; -.
DR MEROPS; A22.A02; -.
DR iPTMnet; Q9SIK7; -.
DR PaxDb; Q9SIK7; -.
DR PRIDE; Q9SIK7; -.
DR ProteomicsDB; 248759; -.
DR EnsemblPlants; AT2G29900.1; AT2G29900.1; AT2G29900.
DR GeneID; 817540; -.
DR Gramene; AT2G29900.1; AT2G29900.1; AT2G29900.
DR KEGG; ath:AT2G29900; -.
DR Araport; AT2G29900; -.
DR TAIR; locus:2052080; AT2G29900.
DR eggNOG; KOG2736; Eukaryota.
DR HOGENOM; CLU_022975_2_0_1; -.
DR InParanoid; Q9SIK7; -.
DR OMA; YEARPVN; -.
DR OrthoDB; 797738at2759; -.
DR PhylomeDB; Q9SIK7; -.
DR PRO; PR:Q9SIK7; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9SIK7; baseline and differential.
DR Genevisible; Q9SIK7; AT.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005798; C:Golgi-associated vesicle; IDA:TAIR.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IDA:TAIR.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0004190; F:aspartic-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0004175; F:endopeptidase activity; IBA:GO_Central.
DR GO; GO:0007219; P:Notch signaling pathway; IEA:UniProtKB-KW.
DR GO; GO:0016485; P:protein processing; IEA:InterPro.
DR Gene3D; 1.10.472.100; -; 1.
DR InterPro; IPR001108; Peptidase_A22A.
DR InterPro; IPR006639; Preselin/SPP.
DR InterPro; IPR042524; Presenilin_C.
DR PANTHER; PTHR10202; PTHR10202; 2.
DR Pfam; PF01080; Presenilin; 2.
DR PRINTS; PR01072; PRESENILIN.
DR SMART; SM00730; PSN; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Golgi apparatus; Membrane; Notch signaling pathway;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..397
FT /note="Presenilin-like protein At2g29900"
FT /id="PRO_0000073906"
FT TOPO_DOM 1..17
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 18..38
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 39..76
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 77..97
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 98..106
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 107..127
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 128..135
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 136..156
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 157..158
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 159..179
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 180..188
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 189..209
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 210..305
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 306..326
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 327..336
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 337..357
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 358..366
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT INTRAMEM 367..387
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 388..397
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOTIF 363..365
FT /note="PAL"
FT ACT_SITE 198
FT /evidence="ECO:0000250"
FT ACT_SITE 318
FT /evidence="ECO:0000250"
SQ SEQUENCE 397 AA; 44012 MW; DE6804D6CA186783 CRC64;
MDRNQRPRSI LDSLGEELIA ILTPVSICMF TVVLLVCILN SDPSSSSASF SSIATAAYSE
SDSDSSWDKF VGALLNSVVF VAAITVATFV LVLLFYLRCV KFLKFYMGFS AFIVLGNLGG
EILVLLIDRF RFPIDSITFL ILLFNFSVVG VFAVFMSKFS ILITQGYLVW IGVLVAYFFT
LLPEWTTWVL LVALALYDIA AVLLPVGPLR LLVEMAISRD EDIPALVYEA RPVIRNDSRS
VQRRVWREQR SSQNNANRNE VRVVESAEVE EEHVGSSERA EISVPLIDRR PEQAENSETF
LEGIGLGSSG AIKLGLGDFI FYSVLVGRAA MYDLMTVYAC YLAIIAGLGI TLMLLSVYQK
ALPALPVSIM LGVVFYFLAR LLLEVFVVQC SSNLVMF
