ID   PSPA_ECO57              Reviewed;         222 AA.
AC   P0AFM7; P23853; P76040; P77363;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=Phage shock protein A;
GN   Name=pspA; OrderedLocusNames=Z2482, ECs1881;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: The phage shock protein (psp) operon (pspABCDE) may play a
CC       significant role in the competition for survival under nutrient- or
CC       energy-limited conditions. PspA negatively regulates expression of the
CC       pspABCDE promoter and of pspG through negative regulation of the psp-
CC       specific transcriptional activator PspF. Is also required for membrane
CC       integrity, efficient translocation and maintenance of the proton motive
CC       force (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Cell inner membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC       side {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the PspA/IM30 family. {ECO:0000305}.
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DR   EMBL; AE005174; AAG56500.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB35304.1; -; Genomic_DNA.
DR   PIR; A99864; A99864.
DR   PIR; H85754; H85754.
DR   RefSeq; NP_309908.1; NC_002695.1.
DR   RefSeq; WP_000511025.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; P0AFM7; -.
DR   SMR; P0AFM7; -.
DR   STRING; 155864.EDL933_2378; -.
DR   EnsemblBacteria; AAG56500; AAG56500; Z2482.
DR   EnsemblBacteria; BAB35304; BAB35304; ECs_1881.
DR   GeneID; 66674868; -.
DR   GeneID; 912684; -.
DR   KEGG; ece:Z2482; -.
DR   KEGG; ecs:ECs_1881; -.
DR   PATRIC; fig|386585.9.peg.1985; -.
DR   eggNOG; COG1842; Bacteria.
DR   HOGENOM; CLU_056466_3_0_6; -.
DR   OMA; MIFRAKA; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR014319; Phageshock_PspA.
DR   InterPro; IPR007157; PspA_IM30.
DR   PANTHER; PTHR31088; PTHR31088; 1.
DR   Pfam; PF04012; PspA_IM30; 1.
DR   TIGRFAMs; TIGR02977; phageshock_pspA; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Coiled coil; Cytoplasm; Membrane;
KW   Reference proteome.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..222
FT                   /note="Phage shock protein A"
FT                   /id="PRO_0000166293"
FT   COILED          30..187
FT                   /evidence="ECO:0000255"
FT   COILED          204..222
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   222 AA;  25493 MW;  EAC29290406E5053 CRC64;
     MGIFSRFADI VNANINALLE KAEDPQKLVR LMIQEMEDTL VEVRSTSARA LAEKKQLTRR
     IEQASAREVE WQEKAELALL KEREDLARAA LIEKQKLTDL IKSLEHEVTL VDDTLARMKK
     EIGELENKLS ETRARQQALM LRHQAANSSR DVRRQLDSGK LDEAMARFES FERRIDQMEA
     EAESHSFGKQ KSLDDQFAEL KADDAISEQL AQLKAKMKQD NQ