ID   PSPA_SHIFL              Reviewed;         222 AA.
AC   P0AFM8; P23853; P76040; P77363;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Phage shock protein A;
GN   Name=pspA; OrderedLocusNames=SF1309, S1391;
OS   Shigella flexneri.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Shigella.
OX   NCBI_TaxID=623;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=301 / Serotype 2a;
RX   PubMed=12384590; DOI=10.1093/nar/gkf566;
RA   Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA   Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA   Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA   Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT   "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT   through comparison with genomes of Escherichia coli K12 and O157.";
RL   Nucleic Acids Res. 30:4432-4441(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX   PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA   Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA   Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA   Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT   "Complete genome sequence and comparative genomics of Shigella flexneri
RT   serotype 2a strain 2457T.";
RL   Infect. Immun. 71:2775-2786(2003).
CC   -!- FUNCTION: The phage shock protein (psp) operon (pspABCDE) may play a
CC       significant role in the competition for survival under nutrient- or
CC       energy-limited conditions. PspA negatively regulates expression of the
CC       pspABCDE promoter and of pspG through negative regulation of the psp-
CC       specific transcriptional activator PspF. Is also required for membrane
CC       integrity, efficient translocation and maintenance of the proton motive
CC       force (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Cell inner membrane
CC       {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Cytoplasmic
CC       side {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the PspA/IM30 family. {ECO:0000305}.
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DR   EMBL; AE005674; AAN42920.1; -; Genomic_DNA.
DR   EMBL; AE014073; AAP16802.1; -; Genomic_DNA.
DR   RefSeq; NP_707213.1; NC_004337.2.
DR   RefSeq; WP_000511025.1; NZ_WPGW01000009.1.
DR   AlphaFoldDB; P0AFM8; -.
DR   SMR; P0AFM8; -.
DR   STRING; 198214.SF1309; -.
DR   PRIDE; P0AFM8; -.
DR   EnsemblBacteria; AAN42920; AAN42920; SF1309.
DR   EnsemblBacteria; AAP16802; AAP16802; S1391.
DR   GeneID; 1024253; -.
DR   GeneID; 66674868; -.
DR   KEGG; sfl:SF1309; -.
DR   KEGG; sfx:S1391; -.
DR   PATRIC; fig|198214.7.peg.1535; -.
DR   HOGENOM; CLU_056466_3_0_6; -.
DR   OMA; MIFRAKA; -.
DR   OrthoDB; 941029at2; -.
DR   Proteomes; UP000001006; Chromosome.
DR   Proteomes; UP000002673; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR014319; Phageshock_PspA.
DR   InterPro; IPR007157; PspA_IM30.
DR   PANTHER; PTHR31088; PTHR31088; 1.
DR   Pfam; PF04012; PspA_IM30; 1.
DR   TIGRFAMs; TIGR02977; phageshock_pspA; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Coiled coil; Cytoplasm; Membrane;
KW   Reference proteome.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..222
FT                   /note="Phage shock protein A"
FT                   /id="PRO_0000166294"
FT   COILED          30..187
FT                   /evidence="ECO:0000255"
FT   COILED          204..222
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   222 AA;  25493 MW;  EAC29290406E5053 CRC64;
     MGIFSRFADI VNANINALLE KAEDPQKLVR LMIQEMEDTL VEVRSTSARA LAEKKQLTRR
     IEQASAREVE WQEKAELALL KEREDLARAA LIEKQKLTDL IKSLEHEVTL VDDTLARMKK
     EIGELENKLS ETRARQQALM LRHQAANSSR DVRRQLDSGK LDEAMARFES FERRIDQMEA
     EAESHSFGKQ KSLDDQFAEL KADDAISEQL AQLKAKMKQD NQ