PSPB_RABIT
ID PSPB_RABIT Reviewed; 370 AA.
AC P15285; P79333;
DT 01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Pulmonary surfactant-associated protein B;
DE Short=SP-B;
DE AltName: Full=6 kDa protein;
DE AltName: Full=Pulmonary surfactant-associated proteolipid SPL(Phe);
DE Flags: Precursor;
GN Name=SFTPB; Synonyms=SFTP3;
OS Oryctolagus cuniculus (Rabbit).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Lagomorpha; Leporidae; Oryctolagus.
OX NCBI_TaxID=9986;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Lung;
RX PubMed=2469419; DOI=10.1016/0006-291x(89)91659-8;
RA Xu J., Richardson C., Ford C., Spencer T., Li-Juan Y., Mackie G.,
RA Hammond G., Possmayer F.;
RT "Isolation and characterization of the cDNA for pulmonary surfactant-
RT associated protein-B (SP-B) in the rabbit.";
RL Biochem. Biophys. Res. Commun. 160:325-332(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=New Zealand white;
RX PubMed=7900830; DOI=10.1152/ajplung.1995.268.3.l481;
RA Margana R.K., Boggaram V.;
RT "Transcription and mRNA stability regulate developmental and hormonal
RT expression of rabbit surfactant protein B gene.";
RL Am. J. Physiol. 268:L481-L490(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TISSUE=Liver;
RX PubMed=8928820; DOI=10.1152/ajplung.1996.270.4.l601;
RA Margana R.K., Boggaram V.;
RT "Rabbit surfactant protein B gene: structure and functional
RT characterization of the promoter.";
RL Am. J. Physiol. 270:L601-L612(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-34.
RX PubMed=8522191; DOI=10.1016/0378-1119(95)00506-2;
RA Luzi P., Anceschi M., Strayer D.S.;
RT "The upstream region of the SP-B gene: intrinsic promoter activity and
RT glucocorticoid responsiveness related to a new DNA-binding protein.";
RL Gene 165:285-290(1995).
CC -!- FUNCTION: Pulmonary surfactant-associated proteins promote alveolar
CC stability by lowering the surface tension at the air-liquid interface
CC in the peripheral air spaces. SP-B increases the collapse pressure of
CC palmitic acid to nearly 70 millinewtons per meter.
CC -!- SUBUNIT: Homodimer; disulfide-linked.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, surface film.
CC -!- MISCELLANEOUS: Pulmonary surfactant consists of 90% lipid and 10%
CC protein. There are 4 surfactant-associated proteins: 2 collagenous,
CC carbohydrate-binding glycoproteins (SP-A and SP-D) and 2 small
CC hydrophobic proteins (SP-B and SP-C).
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DR EMBL; M24901; AAA31466.1; -; mRNA.
DR EMBL; U17106; AAA67934.1; -; mRNA.
DR EMBL; U40853; AAB48076.1; -; Genomic_DNA.
DR EMBL; S80649; AAD14335.1; -; Genomic_DNA.
DR PIR; A32421; LNRBB.
DR PIR; I46531; I46531.
DR RefSeq; NP_001075812.1; NM_001082343.1.
DR RefSeq; XP_008250836.1; XM_008252614.2.
DR RefSeq; XP_008250841.1; XM_008252619.2.
DR RefSeq; XP_008250852.1; XM_008252630.2.
DR AlphaFoldDB; P15285; -.
DR SMR; P15285; -.
DR BioGRID; 1172216; 1.
DR STRING; 9986.ENSOCUP00000025658; -.
DR Ensembl; ENSOCUT00000021287; ENSOCUP00000025658; ENSOCUG00000020733.
DR GeneID; 100009194; -.
DR KEGG; ocu:100009194; -.
DR CTD; 6439; -.
DR eggNOG; KOG1340; Eukaryota.
DR GeneTree; ENSGT00940000161711; -.
DR HOGENOM; CLU_063244_0_0_1; -.
DR InParanoid; P15285; -.
DR OMA; APHTICS; -.
DR OrthoDB; 865505at2759; -.
DR TreeFam; TF316942; -.
DR Proteomes; UP000001811; Chromosome 2.
DR Bgee; ENSOCUG00000020733; Expressed in upper lobe of left lung and 11 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-SubCell.
DR GO; GO:0006629; P:lipid metabolic process; IEA:InterPro.
DR GO; GO:0007585; P:respiratory gaseous exchange by respiratory system; IEA:UniProtKB-KW.
DR InterPro; IPR003119; SAP_A.
DR InterPro; IPR007856; SapB_1.
DR InterPro; IPR008138; SapB_2.
DR InterPro; IPR011001; Saposin-like.
DR InterPro; IPR008139; SaposinB_dom.
DR Pfam; PF02199; SapA; 1.
DR Pfam; PF05184; SapB_1; 1.
DR Pfam; PF03489; SapB_2; 2.
DR SMART; SM00162; SAPA; 1.
DR SMART; SM00741; SapB; 3.
DR SUPFAM; SSF47862; SSF47862; 3.
DR PROSITE; PS51110; SAP_A; 1.
DR PROSITE; PS50015; SAP_B; 3.
PE 2: Evidence at transcript level;
KW Disulfide bond; Gaseous exchange; Glycoprotein; Reference proteome; Repeat;
KW Secreted; Signal; Surface film.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT PROPEP 25..184
FT /id="PRO_0000031653"
FT CHAIN 185..263
FT /note="Pulmonary surfactant-associated protein B"
FT /id="PRO_0000031654"
FT PROPEP 264..370
FT /id="PRO_0000031655"
FT DOMAIN 26..66
FT /note="Saposin A-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00414"
FT DOMAIN 66..148
FT /note="Saposin B-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DOMAIN 188..265
FT /note="Saposin B-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DOMAIN 284..359
FT /note="Saposin B-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT CARBOHYD 300
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 70..144
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 73..138
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 101..113
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 192..261
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 195..255
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 219..230
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 232
FT /note="Interchain"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 288..355
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 291..349
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT DISULFID 314..324
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00415"
FT CONFLICT 15
FT /note="Missing (in Ref. 2; AAA67934)"
FT /evidence="ECO:0000305"
FT CONFLICT 129
FT /note="Q -> L (in Ref. 3; AAB48076)"
FT /evidence="ECO:0000305"
FT CONFLICT 184
FT /note="R -> P (in Ref. 1; AAA31466)"
FT /evidence="ECO:0000305"
FT CONFLICT 232
FT /note="C -> R (in Ref. 2; AAA67934)"
FT /evidence="ECO:0000305"
FT CONFLICT 289
FT /note="R -> P (in Ref. 3; AAB48076)"
FT /evidence="ECO:0000305"
FT CONFLICT 329..355
FT /note="ELHTPQLLSLLSRGWDARAICQALGAC -> AAHAPAAEPAVQGLGCPRNLP
FT GPEGRV (in Ref. 1; AAA31466)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 370 AA; 40610 MW; 423047A69B12DCB5 CRC64;
MAKSHLPPWL LLLLLPTLCG PGTAVWATSP LACAQGPEFW CQSLEQALQC KALGHCLQEV
WGHVGADDLC QECQDIVNIL TKMTKEAIFQ DTIRKFLEHE CDVLPLKLLV PQCHHVLDVY
FPLTITYFQS QINAKAICQH LGLCQPGSPE PPLDPLPDKL VLPTLLGALP AKPGPHTQDL
SAQRFPIPLP LCWLCRTLLK RIQAMIPKGV LAMAVAQVCH VVPLVVGGIC QCLAERYTVI
LLEVLLGHVL PQLVCGLVLR CSSVDSIGQV PPTLEALPGE WLPQDPECRL CMSVTTQARN
ISEQTRPQAV YHACLSSQLD KQECEQFVEL HTPQLLSLLS RGWDARAICQ ALGACVATLS
PLQCIQSPHF
