PSPE_DICDI
ID PSPE_DICDI Reviewed; 155 AA.
AC Q9GP84; Q54L41;
DT 08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 65.
DE RecName: Full=Prespore-specific protein E;
DE AltName: Full=Protein 1I;
DE Flags: Precursor;
GN Name=pspE; ORFNames=DDB_G0286905;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, AND GPI-ANCHOR AT ASN-133.
RX PubMed=11286975; DOI=10.1016/s0005-2736(01)00289-9;
RA Stevens B.A., White I.J., Hames B.D., Hooper N.M.;
RT "The carboxyl terminus of Dictyostelium discoideum protein 1I encodes a
RT functional glycosyl-phosphatidylinositol signal sequence.";
RL Biochim. Biophys. Acta 1511:317-329(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor, GPI-anchor.
CC -!- DEVELOPMENTAL STAGE: Expressed in the prespore cells at culminating
CC stage. {ECO:0000269|PubMed:11286975}.
CC -!- PTM: The GPI-like-anchor contains a phosphoceramide group, rather than
CC a phosphatidyl group.
CC -!- CAUTION: The Dictyosteliida are known to produce a
CC glycosylsphingolipidinositol anchor (GPI-like-anchor). It has not been
CC established whether Dictyosteliida make a glycosylphosphatidylinositol
CC anchor (GPI-anchor) also, and whether their GPI-like-anchor
CC modifications can be interconverted with GPI-anchor modifications in a
CC resculpting process. It has not been established that the GPI-like-
CC anchor modification in Dictyosteliida utilizes the same sequence motif.
CC {ECO:0000305}.
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DR EMBL; AJ292240; CAC21551.1; -; mRNA.
DR EMBL; AAFI02000092; EAL63950.1; -; Genomic_DNA.
DR RefSeq; XP_637462.1; XM_632370.1.
DR AlphaFoldDB; Q9GP84; -.
DR STRING; 44689.DDB0191248; -.
DR PaxDb; Q9GP84; -.
DR EnsemblProtists; EAL63950; EAL63950; DDB_G0286905.
DR GeneID; 8625861; -.
DR KEGG; ddi:DDB_G0286905; -.
DR dictyBase; DDB_G0286905; pspE.
DR HOGENOM; CLU_1698779_0_0_1; -.
DR InParanoid; Q9GP84; -.
DR OMA; GFIFTEY; -.
DR PRO; PR:Q9GP84; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0031225; C:anchored component of membrane; TAS:dictyBase.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
PE 1: Evidence at protein level;
KW Cell membrane; Glycoprotein; GPI-anchor; Lipoprotein; Membrane;
KW Reference proteome; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..133
FT /note="Prespore-specific protein E"
FT /id="PRO_0000327478"
FT PROPEP 134..155
FT /note="Removed in mature form"
FT /id="PRO_0000327479"
FT LIPID 133
FT /note="GPI-like-anchor amidated asparagine"
FT /evidence="ECO:0000269|PubMed:11286975"
FT CARBOHYD 22
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 82
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 85
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 102
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 105
FT /note="O-linked (GlcNAc) serine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 155 AA; 16273 MW; 73939B00D3ADE336 CRC64;
MRFISIFLII VALCVSSSWA FNFTDQPNSF RISGTGCGSG TTTVYFSTDG RCNSACGGSI
RIKGEGNNVP NQQFTLNDYS KNVTNCSGTS NVASFRCPAL VNTTSPTFTV NVGNSAYHVT
CQYAQVTETP AGNSADKVAV GIAIIFGALI SLLAL
