PSR1_PHYSO
ID PSR1_PHYSO Reviewed; 106 AA.
AC E0W5Q5;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 02-NOV-2010, sequence version 1.
DT 25-MAY-2022, entry version 27.
DE RecName: Full=RxLR effector protein PSR1 {ECO:0000303|PubMed:21653195};
DE AltName: Full=Avirulence homolog protein 18 {ECO:0000303|PubMed:21653195};
DE AltName: Full=Suppressor of RNA silencing protein 1 {ECO:0000303|PubMed:23377181};
DE Flags: Precursor;
GN Name=PSR1 {ECO:0000303|PubMed:23377181};
GN Synonyms=Avh18 {ECO:0000303|PubMed:21653195};
OS Phytophthora sojae (Soybean stem and root rot agent) (Phytophthora
OS megasperma f. sp. glycines).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=67593;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DOMAIN.
RC STRAIN=P7074, and P7076;
RX PubMed=21653195; DOI=10.1105/tpc.111.086082;
RA Wang Q., Han C., Ferreira A.O., Yu X., Ye W., Tripathy S., Kale S.D.,
RA Gu B., Sheng Y., Sui Y., Wang X., Zhang Z., Cheng B., Dong S., Shan W.,
RA Zheng X., Dou D., Tyler B.M., Wang Y.;
RT "Transcriptional programming and functional interactions within the
RT Phytophthora sojae RXLR effector repertoire.";
RL Plant Cell 23:2064-2086(2011).
RN [2]
RP FUNCTION, DOMAIN, SUBCELLULAR LOCATION, AND MUTAGENESIS OF 56-ARG--PHE-69.
RX PubMed=23377181; DOI=10.1038/ng.2525;
RA Qiao Y., Liu L., Xiong Q., Flores C., Wong J., Shi J., Wang X., Liu X.,
RA Xiang Q., Jiang S., Zhang F., Wang Y., Judelson H.S., Chen X., Ma W.;
RT "Oomycete pathogens encode RNA silencing suppressors.";
RL Nat. Genet. 45:330-333(2013).
RN [3]
RP FUNCTION, AND INTERACTION WITH HOST PINP1.
RX PubMed=25902521; DOI=10.1073/pnas.1421475112;
RA Qiao Y., Shi J., Zhai Y., Hou Y., Ma W.;
RT "Phytophthora effector targets a novel component of small RNA pathway in
RT plants to promote infection.";
RL Proc. Natl. Acad. Sci. U.S.A. 112:5850-5855(2015).
RN [4]
RP FUNCTION, DOMAIN, AND MUTAGENESIS OF TRP-72 AND TYR-100.
RX PubMed=30963588; DOI=10.1111/nph.15836;
RA Zhang P., Jia Y., Shi J., Chen C., Ye W., Wang Y., Ma W., Qiao Y.;
RT "The WY domain in the Phytophthora effector PSR1 is required for infection
RT and RNA silencing suppression activity.";
RL New Phytol. 223:839-852(2019).
CC -!- FUNCTION: Secreted effector that possesses RNA silencing suppression
CC activity by inhibiting the biogenesis of small RNAs in the host plant
CC to promote enhanced susceptibility of host to the pathogen during
CC infection (PubMed:23377181, PubMed:25902521, PubMed:30963588).
CC Interferes with secondary siRNA production by associating with host
CC nuclear protein PINP1 that acts as a regulator of the accumulation of
CC both microRNAs and endogenous small interfering RNAs (PubMed:25902521).
CC {ECO:0000269|PubMed:23377181, ECO:0000269|PubMed:25902521,
CC ECO:0000269|PubMed:30963588}.
CC -!- SUBUNIT: Interacts with host PINP1. {ECO:0000269|PubMed:25902521}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:25902521}. Host
CC nucleus {ECO:0000269|PubMed:25902521}. Note=The nuclear localization is
CC required for this interaction. {ECO:0000269|PubMed:25902521}.
CC -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC phosphate. {ECO:0000305|PubMed:21653195}.
CC -!- DOMAIN: The nuclear localization signal (NLS) is required for
CC localization to the host nucleus and RNA silencing suppression
CC activity. {ECO:0000269|PubMed:23377181}.
CC -!- DOMAIN: The single WY domain is required for binding to host PINP1 and
CC subsequent RNA-silencing suppression activity, pathogenicity and
CC perturbation of plant development. {ECO:0000269|PubMed:30963588}.
CC -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR EMBL; JN253674; AEK80487.1; -; Genomic_DNA.
DR EMBL; JN253675; AEK80488.1; -; Genomic_DNA.
DR RefSeq; XP_009518240.1; XM_009519945.1.
DR AlphaFoldDB; E0W5Q5; -.
DR GeneID; 20639842; -.
DR KEGG; psoj:PHYSODRAFT_284677; -.
DR VEuPathDB; FungiDB:PHYSODRAFT_284677; -.
DR HOGENOM; CLU_2228539_0_0_1; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0042025; C:host cell nucleus; IEA:UniProtKB-SubCell.
DR InterPro; IPR031825; RXLR.
DR Pfam; PF16810; RXLR; 1.
PE 1: Evidence at protein level;
KW Host nucleus; Secreted; Signal; Virulence.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..106
FT /note="RxLR effector protein PSR1"
FT /id="PRO_5007652867"
FT REGION 50..106
FT /note="WY domain"
FT /evidence="ECO:0000305|PubMed:30963588"
FT MOTIF 33..46
FT /note="RxLR-dEER"
FT /evidence="ECO:0000305|PubMed:21653195"
FT MOTIF 56..69
FT /note="Bipartite nuclear localization signal (NLS)"
FT /evidence="ECO:0000269|PubMed:23377181"
FT MUTAGEN 56..69
FT /note="RKMLGDETYRLKKF->AAAAAAAAAAAAAA: Loses the nuclear
FT localization as well as the RNA silencing 3suppression
FT activity."
FT /evidence="ECO:0000269|PubMed:23377181"
FT MUTAGEN 72
FT /note="W->A: Abolishes the interaction with host target
FT PINP1 and lowers the size of lesions in leaves after
FT infection."
FT /evidence="ECO:0000269|PubMed:30963588"
FT MUTAGEN 100
FT /note="Y->A: LAbolishes the interaction with host target
FT PINP1 and lowers the size of lesions in leaves after
FT infection."
FT /evidence="ECO:0000269|PubMed:30963588"
SQ SEQUENCE 106 AA; 12304 MW; DCDE91B059ABF2CF CRC64;
MRLTYVLLVA VTTLLVSCDA TKPSTEATAV SKRLLRFVEA ADEEERRIDF SPEKLRKMLG
DETYRLKKFG KWDSDGHTFD GLKHYLLLSD SSMVKLRNMY KAWLEQ
