PSR2_PHYIT
ID PSR2_PHYIT Reviewed; 760 AA.
AC D0NRS4;
DT 16-OCT-2019, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2009, sequence version 1.
DT 03-AUG-2022, entry version 35.
DE RecName: Full=RxLR effector protein PSR2 {ECO:0000303|PubMed:25387135};
DE AltName: Full=Suppressor of RNA silencing protein 2 {ECO:0000303|PubMed:25387135};
DE Flags: Precursor;
GN Name=PSR2 {ECO:0000303|PubMed:25387135}; ORFNames=PITG_15152;
OS Phytophthora infestans (strain T30-4) (Potato late blight agent).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=403677;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=T30-4;
RX PubMed=19741609; DOI=10.1038/nature08358;
RG The Broad Institute Genome Sequencing Platform;
RA Haas B.J., Kamoun S., Zody M.C., Jiang R.H., Handsaker R.E., Cano L.M.,
RA Grabherr M., Kodira C.D., Raffaele S., Torto-Alalibo T., Bozkurt T.O.,
RA Ah-Fong A.M., Alvarado L., Anderson V.L., Armstrong M.R., Avrova A.,
RA Baxter L., Beynon J., Boevink P.C., Bollmann S.R., Bos J.I., Bulone V.,
RA Cai G., Cakir C., Carrington J.C., Chawner M., Conti L., Costanzo S.,
RA Ewan R., Fahlgren N., Fischbach M.A., Fugelstad J., Gilroy E.M., Gnerre S.,
RA Green P.J., Grenville-Briggs L.J., Griffith J., Grunwald N.J., Horn K.,
RA Horner N.R., Hu C.H., Huitema E., Jeong D.H., Jones A.M., Jones J.D.,
RA Jones R.W., Karlsson E.K., Kunjeti S.G., Lamour K., Liu Z., Ma L.,
RA Maclean D., Chibucos M.C., McDonald H., McWalters J., Meijer H.J.,
RA Morgan W., Morris P.F., Munro C.A., O'Neill K., Ospina-Giraldo M.,
RA Pinzon A., Pritchard L., Ramsahoye B., Ren Q., Restrepo S., Roy S.,
RA Sadanandom A., Savidor A., Schornack S., Schwartz D.C., Schumann U.D.,
RA Schwessinger B., Seyer L., Sharpe T., Silvar C., Song J., Studholme D.J.,
RA Sykes S., Thines M., van de Vondervoort P.J., Phuntumart V., Wawra S.,
RA Weide R., Win J., Young C., Zhou S., Fry W., Meyers B.C., van West P.,
RA Ristaino J., Govers F., Birch P.R., Whisson S.C., Judelson H.S.,
RA Nusbaum C.;
RT "Genome sequence and analysis of the Irish potato famine pathogen
RT Phytophthora infestans.";
RL Nature 461:393-398(2009).
RN [2]
RP FUNCTION.
RX PubMed=25387135; DOI=10.1094/mpmi-06-14-0190-r;
RA Xiong Q., Ye W., Choi D., Wong J., Qiao Y., Tao K., Wang Y., Ma W.;
RT "Phytophthora suppressor of RNA silencing 2 is a conserved RxLR effector
RT that promotes infection in soybean and Arabidopsis thaliana.";
RL Mol. Plant Microbe Interact. 27:1379-1389(2014).
RN [3]
RP FUNCTION, AND INDUCTION.
RX PubMed=27503598; DOI=10.1111/mpp.12465;
RA de Vries S., von Dahlen J.K., Uhlmann C., Schnake A., Kloesges T.,
RA Rose L.E.;
RT "Signatures of selection and host-adapted gene expression of the
RT Phytophthora infestans RNA silencing suppressor PSR2.";
RL Mol. Plant Pathol. 18:110-124(2017).
CC -!- FUNCTION: Secreted effector that possesses RNA silencing suppression
CC activity by inhibiting the biogenesis of small RNAs in the host plant
CC to promote enhanced susceptibility of host to the pathogen during
CC infection (PubMed:25387135, PubMed:27503598). Interferes with secondary
CC siRNA production by associating with host dsRNA-binding protein DRB4
CC (By similarity). Inhibits the host salicylic acid pathway during
CC infection (By similarity). {ECO:0000250|UniProtKB:E0W4V5,
CC ECO:0000269|PubMed:25387135, ECO:0000269|PubMed:27503598}.
CC -!- SUBUNIT: Interacts with host dsRNA-binding protein DRB4.
CC {ECO:0000250|UniProtKB:E0W4V5}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:25387135}. Host cell
CC {ECO:0000305|PubMed:25387135}.
CC -!- INDUCTION: The expression is highest in the biotrophic phase of the
CC pathogen. {ECO:0000269|PubMed:27503598}.
CC -!- DOMAIN: The RxLR-dEER motif acts to carry the protein into the host
CC cell cytoplasm through binding to cell surface phosphatidylinositol-3-
CC phosphate. {ECO:0000305|PubMed:25387135}.
CC -!- DOMAIN: The C-terminal region (residues 87 to 760) consists of seven
CC imperfect tandem repeats, including one W-Y motif (WY1) and six L-W-Y
CC motifs (LWY2 to LWY7) (By similarity). WY1 forms a 3 alpha-helix fold
CC with one hydrophobic core and each L-W-Y motif forms a highly conserved
CC fold consisting of 5 alpha-helices (By similarity). The units
CC contribute differently to the virulence since WY1, LWY2 and LWY6 are
CC important for the ability to suppress the biogenesis of small RNA in
CC host and virulence activity of the pathogen, whereas LWY3, LWY4, LWY5
CC and LWY7 are dispensable for PSR2 function (By similarity). WY1 and
CC LWY2 are sufficient for association with DRB4, suppress gene silencing
CC and promote infection (By similarity). These units may function as
CC basic building blocks of Phytophthora effectors to enable virulence
CC activity and accelerate the evolution of novel functions (By
CC similarity). {ECO:0000250|UniProtKB:E0W4V5}.
CC -!- SIMILARITY: Belongs to the RxLR effector family. {ECO:0000305}.
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DR EMBL; DS028155; EEY63424.1; -; Genomic_DNA.
DR RefSeq; XP_002898309.1; XM_002898263.1.
DR AlphaFoldDB; D0NRS4; -.
DR SMR; D0NRS4; -.
DR EnsemblProtists; PITG_15152T0; PITG_15152T0; PITG_15152.
DR GeneID; 9476029; -.
DR KEGG; pif:PITG_15152; -.
DR VEuPathDB; FungiDB:PITG_15152; -.
DR eggNOG; ENOG502SS1M; Eukaryota.
DR HOGENOM; CLU_021192_3_0_1; -.
DR InParanoid; D0NRS4; -.
DR OMA; TLYMKTD; -.
DR OrthoDB; 717762at2759; -.
DR Proteomes; UP000006643; Partially assembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0043657; C:host cell; IEA:UniProtKB-SubCell.
PE 2: Evidence at transcript level;
KW Reference proteome; Repeat; Secreted; Signal; Virulence.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..760
FT /note="RxLR effector protein PSR2"
FT /id="PRO_5003012325"
FT REPEAT 87..134
FT /note="WY1"
FT /evidence="ECO:0000250|UniProtKB:E0W4V5"
FT REPEAT 135..221
FT /note="LWY2"
FT /evidence="ECO:0000250|UniProtKB:E0W4V5"
FT REPEAT 222..312
FT /note="LWY3"
FT /evidence="ECO:0000250|UniProtKB:E0W4V5"
FT REPEAT 313..403
FT /note="LWY4"
FT /evidence="ECO:0000250|UniProtKB:E0W4V5"
FT REPEAT 404..496
FT /note="LWY5"
FT /evidence="ECO:0000250|UniProtKB:E0W4V5"
FT REPEAT 497..584
FT /note="LWY6"
FT /evidence="ECO:0000250|UniProtKB:E0W4V5"
FT REPEAT 585..760
FT /note="LWY7"
FT /evidence="ECO:0000250|UniProtKB:E0W4V5"
FT REGION 87..760
FT /note="7 X 93 AA tandem repeats"
FT /evidence="ECO:0000250|UniProtKB:E0W4V5"
FT MOTIF 47..62
FT /note="RxLR-dEER"
FT /evidence="ECO:0000305|PubMed:25387135"
SQ SEQUENCE 760 AA; 85357 MW; EF18FB76860CCD04 CRC64;
MGCRYAVLAL AVAYFAGSIA ANDSQIVAVK GPASIRFTPA IHVVRGRFLR AANTADERNE
DRGINLKSMP GFEKIASLFT KKNTPGPLLS WFEKKKSPDY VFLKLKINKG KQQLFDHPDW
NVWVQYTTSV VKSDPEEAMI AALRTHYTDD ILSKLLESAK NVPKTSGLAT KMQMEHWVAS
KTPSQMFQFL RLDKVRNGVL DDPTLSIWIN YMKLYNSKPV NKKQQVTLVS MLTTHYKDRG
VLDIIEAAKK VPKTAPAARQ LEMEQIQFWL KNGKSPDELL TVLSLDKAGN QLLASPRFKF
WSKYVDNYNR DFPDEATTVM ATLRNQLGDE DITPILIAAG KVPSTEKAAA KLQAEQFKSW
LRENEDPAKV FQLLKLDNSA DDLLGSPQFK LWGKYVEDLN LKPEHNDLQV SIITILRKNY
GDDVLGNMVL AGKKAPSTSF MARRLEDELY KGWIAAGSSP DGVFKHLKFD KAGENVIQSP
LWGLYTKFLE HYYKSFPTPM MSALAKGYDG DALAKLLIAA EKIPTSNTLA TKLQTGQIQR
WLDDKDQPGK IFKALLLDDM ADDILTSPLF NTWTRYLDEF NKKFPDEKVS MTDTFRTSLD
DETLKSLLIT AKELPDMKTL STKLQTVQIE RWLASKTSPE DAFAVLALNK AGGNVLSKPL
LNTWAAYLES FNAKFPRSRV SMIDTFREFF GDKALLTTLA AAKEVESTKK VATSLQDSLL
SKWVLAKKPP SGVAKLVGTD EAGAKLLKTY TTKYMERYGQ
