ATNG_MOUSE
ID ATNG_MOUSE Reviewed; 70 AA.
AC Q04646; Q923I2; Q923I3; Q923I4;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2002, sequence version 2.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Sodium/potassium-transporting ATPase subunit gamma;
DE Short=Na(+)/K(+) ATPase subunit gamma;
DE AltName: Full=FXYD domain-containing ion transport regulator 2;
DE AltName: Full=Sodium pump gamma chain;
GN Name=Fxyd2; Synonyms=Atp1c, Atp1g1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS 1; 2 AND 3).
RC STRAIN=129/Sv;
RX PubMed=11526196; DOI=10.1152/physiolgenomics.2001.6.3.129;
RA Jones D.H., Golding M.C., Barr K.J., Fong G.-H., Kidder G.M.;
RT "The mouse Na+-K+-ATPase gamma-subunit gene (Fxyd2) encodes three
RT developmentally regulated transcripts.";
RL Physiol. Genomics 6:129-135(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Kidney;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=FVB/N; TISSUE=Kidney;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 18-70.
RC TISSUE=Kidney;
RX PubMed=8387529; DOI=10.1083/jcb.121.3.579;
RA Mercer R.W., Biemesderfer D., Bliss D.P. Jr., Collins J.H., Forbush B. III;
RT "Molecular cloning and immunological characterization of the gamma
RT polypeptide, a small protein associated with the Na,K-ATPase.";
RL J. Cell Biol. 121:579-586(1993).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Kidney;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May be involved in forming the receptor site for cardiac
CC glycoside binding or may modulate the transport function of the sodium
CC ATPase.
CC -!- SUBUNIT: Regulatory subunit of the sodium/potassium-transporting ATPase
CC which is composed of a catalytic alpha subunit, an auxiliary non-
CC catalytic beta subunit and an additional regulatory subunit.
CC {ECO:0000250|UniProtKB:O13001}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type III
CC membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1; Synonyms=A;
CC IsoId=Q04646-1; Sequence=Displayed;
CC Name=2; Synonyms=B;
CC IsoId=Q04646-2; Sequence=VSP_001581;
CC Name=3; Synonyms=C;
CC IsoId=Q04646-3; Sequence=VSP_001582;
CC -!- TISSUE SPECIFICITY: Highest levels expressed in the kidney and spleen.
CC Restricted to the basolateral membrane in renal epithelial cells and
CC varies in its level of expression along the nephron.
CC -!- SIMILARITY: Belongs to the FXYD family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA49664.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AY035583; AAK62036.1; -; Genomic_DNA.
DR EMBL; AY035583; AAK62037.1; -; Genomic_DNA.
DR EMBL; AY035583; AAK62038.1; -; Genomic_DNA.
DR EMBL; AK002215; BAC24981.1; -; mRNA.
DR EMBL; BC024614; AAH24614.1; -; mRNA.
DR EMBL; X70060; CAA49664.1; ALT_INIT; mRNA.
DR CCDS; CCDS23134.1; -. [Q04646-2]
DR PIR; C46435; C46435.
DR RefSeq; NP_031529.2; NM_007503.3. [Q04646-1]
DR RefSeq; NP_439888.1; NM_052823.2. [Q04646-2]
DR AlphaFoldDB; Q04646; -.
DR ComplexPortal; CPX-126; Sodium:potassium-exchanging ATPase complex.
DR STRING; 10090.ENSMUSP00000035429; -.
DR SwissPalm; Q04646; -.
DR jPOST; Q04646; -.
DR MaxQB; Q04646; -.
DR PaxDb; Q04646; -.
DR PeptideAtlas; Q04646; -.
DR PRIDE; Q04646; -.
DR ProteomicsDB; 277193; -. [Q04646-1]
DR ProteomicsDB; 277194; -. [Q04646-2]
DR ProteomicsDB; 277195; -. [Q04646-3]
DR DNASU; 11936; -.
DR Ensembl; ENSMUST00000041005; ENSMUSP00000035429; ENSMUSG00000059412. [Q04646-2]
DR GeneID; 11936; -.
DR KEGG; mmu:11936; -.
DR UCSC; uc009pfv.1; mouse. [Q04646-1]
DR CTD; 486; -.
DR MGI; MGI:1195260; Fxyd2.
DR VEuPathDB; HostDB:ENSMUSG00000059412; -.
DR eggNOG; ENOG502SGMI; Eukaryota.
DR GeneTree; ENSGT00940000153062; -.
DR HOGENOM; CLU_171208_4_0_1; -.
DR InParanoid; Q04646; -.
DR OMA; HRQVNED; -.
DR PhylomeDB; Q04646; -.
DR Reactome; R-MMU-5578775; Ion homeostasis.
DR Reactome; R-MMU-936837; Ion transport by P-type ATPases.
DR BioGRID-ORCS; 11936; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Fxyd2; mouse.
DR PRO; PR:Q04646; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q04646; protein.
DR Bgee; ENSMUSG00000059412; Expressed in right kidney and 238 other tissues.
DR ExpressionAtlas; Q04646; baseline and differential.
DR Genevisible; Q04646; MM.
DR GO; GO:0016323; C:basolateral plasma membrane; ISO:MGI.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0016020; C:membrane; IDA:MGI.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0005890; C:sodium:potassium-exchanging ATPase complex; ISO:MGI.
DR GO; GO:0017080; F:sodium channel regulator activity; IMP:MGI.
DR GO; GO:0010248; P:establishment or maintenance of transmembrane electrochemical gradient; IC:ComplexPortal.
DR GO; GO:0098662; P:inorganic cation transmembrane transport; ISO:MGI.
DR GO; GO:0008285; P:negative regulation of cell population proliferation; ISO:MGI.
DR GO; GO:1903407; P:negative regulation of P-type sodium:potassium-exchanging transporter activity; ISO:MGI.
DR GO; GO:0006813; P:potassium ion transport; IEA:UniProtKB-KW.
DR GO; GO:1902600; P:proton transmembrane transport; IC:ComplexPortal.
DR GO; GO:1903406; P:regulation of P-type sodium:potassium-exchanging transporter activity; ISO:MGI.
DR GO; GO:2000649; P:regulation of sodium ion transmembrane transporter activity; IMP:MGI.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR000272; Ion-transport_regulator_FXYD.
DR Pfam; PF02038; ATP1G1_PLM_MAT8; 1.
DR PROSITE; PS01310; FXYD; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Ion transport; Membrane; Potassium;
KW Potassium transport; Reference proteome; Sodium; Sodium transport;
KW Sodium/potassium transport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..70
FT /note="Sodium/potassium-transporting ATPase subunit gamma"
FT /id="PRO_0000148186"
FT TRANSMEM 33..50
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..12
FT /note="MAGEISDLSANS -> MDRWYL (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_001581"
FT VAR_SEQ 1..11
FT /note="MAGEISDLSAN -> MQINTKRRKRCWSLKFDALDSGWKPHKALGCWAAESC
FT CRKTG (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_001582"
SQ SEQUENCE 70 AA; 7520 MW; 7114553B004A3422 CRC64;
MAGEISDLSA NSGGSAKGTE NPFEYDYETV RKGGLIFAGL AFVVGLLIIL SKRFRCGGGK
KHRQVNEDEL
