ATNN_EMENI
ID ATNN_EMENI Reviewed; 610 AA.
AC C8V3N0; Q5AV08;
DT 23-MAY-2018, integrated into UniProtKB/Swiss-Prot.
DT 03-NOV-2009, sequence version 1.
DT 25-MAY-2022, entry version 55.
DE RecName: Full=Aspercryptin biosynthesis cluster-specific transcription regulator atnN {ECO:0000303|PubMed:27310134};
DE AltName: Full=Aspercryptin biosynthesis cluster protein N {ECO:0000303|PubMed:26563584};
GN Name=atnN {ECO:0000303|PubMed:26563584}; ORFNames=ANIA_07872;
OS Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 /
OS M139) (Aspergillus nidulans).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC Aspergillus subgen. Nidulantes.
OX NCBI_TaxID=227321;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=16372000; DOI=10.1038/nature04341;
RA Galagan J.E., Calvo S.E., Cuomo C., Ma L.-J., Wortman J.R., Batzoglou S.,
RA Lee S.-I., Bastuerkmen M., Spevak C.C., Clutterbuck J., Kapitonov V.,
RA Jurka J., Scazzocchio C., Farman M.L., Butler J., Purcell S., Harris S.,
RA Braus G.H., Draht O., Busch S., D'Enfert C., Bouchier C., Goldman G.H.,
RA Bell-Pedersen D., Griffiths-Jones S., Doonan J.H., Yu J., Vienken K.,
RA Pain A., Freitag M., Selker E.U., Archer D.B., Penalva M.A., Oakley B.R.,
RA Momany M., Tanaka T., Kumagai T., Asai K., Machida M., Nierman W.C.,
RA Denning D.W., Caddick M.X., Hynes M., Paoletti M., Fischer R., Miller B.L.,
RA Dyer P.S., Sachs M.S., Osmani S.A., Birren B.W.;
RT "Sequencing of Aspergillus nidulans and comparative analysis with A.
RT fumigatus and A. oryzae.";
RL Nature 438:1105-1115(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=FGSC A4 / ATCC 38163 / CBS 112.46 / NRRL 194 / M139;
RX PubMed=19146970; DOI=10.1016/j.fgb.2008.12.003;
RA Wortman J.R., Gilsenan J.M., Joardar V., Deegan J., Clutterbuck J.,
RA Andersen M.R., Archer D., Bencina M., Braus G., Coutinho P., von Dohren H.,
RA Doonan J., Driessen A.J., Durek P., Espeso E., Fekete E., Flipphi M.,
RA Estrada C.G., Geysens S., Goldman G., de Groot P.W., Hansen K.,
RA Harris S.D., Heinekamp T., Helmstaedt K., Henrissat B., Hofmann G.,
RA Homan T., Horio T., Horiuchi H., James S., Jones M., Karaffa L.,
RA Karanyi Z., Kato M., Keller N., Kelly D.E., Kiel J.A., Kim J.M.,
RA van der Klei I.J., Klis F.M., Kovalchuk A., Krasevec N., Kubicek C.P.,
RA Liu B., Maccabe A., Meyer V., Mirabito P., Miskei M., Mos M., Mullins J.,
RA Nelson D.R., Nielsen J., Oakley B.R., Osmani S.A., Pakula T., Paszewski A.,
RA Paulsen I., Pilsyk S., Pocsi I., Punt P.J., Ram A.F., Ren Q., Robellet X.,
RA Robson G., Seiboth B., van Solingen P., Specht T., Sun J.,
RA Taheri-Talesh N., Takeshita N., Ussery D., vanKuyk P.A., Visser H.,
RA van de Vondervoort P.J., de Vries R.P., Walton J., Xiang X., Xiong Y.,
RA Zeng A.P., Brandt B.W., Cornell M.J., van den Hondel C.A., Visser J.,
RA Oliver S.G., Turner G.;
RT "The 2008 update of the Aspergillus nidulans genome annotation: a community
RT effort.";
RL Fungal Genet. Biol. 46:S2-13(2009).
RN [3]
RP IDENTIFICATION OF THE CLUSTER.
RX PubMed=23248299; DOI=10.1073/pnas.1205532110;
RA Andersen M.R., Nielsen J.B., Klitgaard A., Petersen L.M., Zachariasen M.,
RA Hansen T.J., Blicher L.H., Gotfredsen C.H., Larsen T.O., Nielsen K.F.,
RA Mortensen U.H.;
RT "Accurate prediction of secondary metabolite gene clusters in filamentous
RT fungi.";
RL Proc. Natl. Acad. Sci. U.S.A. 110:E99-E107(2013).
RN [4]
RP FUNCTION.
RX PubMed=27310134; DOI=10.1021/acschembio.6b00398;
RA Henke M.T., Soukup A.A., Goering A.W., McClure R.A., Thomson R.J.,
RA Keller N.P., Kelleher N.L.;
RT "New aspercryptins, lipopeptide natural products, revealed by HDAC
RT inhibition in Aspergillus nidulans.";
RL ACS Chem. Biol. 11:2117-2123(2016).
RN [5]
RP FUNCTION.
RX PubMed=26563584; DOI=10.1002/anie.201507097;
RA Chiang Y.M., Ahuja M., Oakley C.E., Entwistle R., Asokan A., Zutz C.,
RA Wang C.C., Oakley B.R.;
RT "Development of genetic dereplication strains in Aspergillus nidulans
RT results in the discovery of aspercryptin.";
RL Angew. Chem. Int. Ed. 55:1662-1665(2016).
CC -!- FUNCTION: Transcription factor that positively regulates the cluster
CC that mediate the production of aspercryptins, linear lipopeptides built
CC from six amino acids including 2 highly unusual and nonproteogenic
CC amino acids, 2-amino-octanoic acid (2aoa) and 2-amino-dodecanol (2adol)
CC (PubMed:27310134, PubMed:26563584). {ECO:0000269|PubMed:27310134,
CC ECO:0000305|PubMed:26563584}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EAA58917.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; BN001302; CBF73428.1; -; Genomic_DNA.
DR EMBL; AACD01000134; EAA58917.1; ALT_SEQ; Genomic_DNA.
DR RefSeq; XP_681141.1; XM_676049.1.
DR AlphaFoldDB; C8V3N0; -.
DR SMR; C8V3N0; -.
DR EnsemblFungi; CBF73428; CBF73428; ANIA_07872.
DR EnsemblFungi; EAA58917; EAA58917; AN7872.2.
DR GeneID; 2869291; -.
DR KEGG; ani:AN7872.2; -.
DR VEuPathDB; FungiDB:AN7872; -.
DR eggNOG; ENOG502SQ3E; Eukaryota.
DR HOGENOM; CLU_366813_0_0_1; -.
DR InParanoid; C8V3N0; -.
DR OMA; YICATRC; -.
DR OrthoDB; 833365at2759; -.
DR Proteomes; UP000000560; Chromosome II.
DR Proteomes; UP000005890; Unassembled WGS sequence.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR CDD; cd00067; GAL4; 1.
DR Gene3D; 4.10.240.10; -; 1.
DR InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR Pfam; PF00172; Zn_clus; 1.
DR SMART; SM00066; GAL4; 1.
DR SUPFAM; SSF57701; SSF57701; 1.
DR PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE 3: Inferred from homology;
KW DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation; Zinc.
FT CHAIN 1..610
FT /note="Aspercryptin biosynthesis cluster-specific
FT transcription regulator atnN"
FT /id="PRO_0000444133"
FT DNA_BIND 30..57
FT /note="Zn(2)-C6 fungal-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 61..81
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 427..493
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 61..78
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 427..492
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 610 AA; 68177 MW; A64AA9ECF4281164 CRC64;
MAPKDSQVSA SNEMTGNPPS SVQGRSRNGC ITCRIRRVKC DEERPHCRRC QSTGRKCDGY
TPLTGQQPKQ QPPQQAAKAG SSELRIIQHT PQVTQPTQLC MFPGVDTLLT EDEYRALEFF
NVQTVSCFGP RAGGWLLNAA CQDSAIRRAA MALGTMHRVV LYHSRTPPHD RRRGMQLALQ
QYNSAIRQGL KLFAGSNDSS ADGILSMCVL FFCLDSLQGH FRSALRHVGS GLRILAQRQL
RGQRAENTLL PPDVIQSLFA ALEAQMLEID GQSPLLDENG LPVRGAGRPA PLWTLEEAQD
TFRSIYNDFL RLLSFSARLE EPVDELEMVQ IVEQVMARKQ QVQTDLDAWS LEFDHFLAHI
FHWGNQASQQ SVRMLQLWRT MLTMVLHMGW PPQDTAWGSH LSELNIILDL AEQIIVMSPP
LELESSAGST FSLQGHSRAG SPSGSRSRSM STSSSASRDD SPTTTTTTTT TPTPRLKKET
DPQSTYTPIL PRPFHSSPSR FTLALGILPA LWTIATQCRD SSVRYRAIDL IGRSKRREGV
WDSDLHFRLA LQLARHEEQA AGLDAGAEYT HARIPPEARV TLNGRFDEGR KAKISYIREN
VRVGEEIFHW
