PST2_YEAST
ID PST2_YEAST Reviewed; 198 AA.
AC Q12335; D6VS16;
DT 26-SEP-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 186.
DE RecName: Full=Protoplast secreted protein 2;
DE Flags: Precursor;
GN Name=PST2; OrderedLocusNames=YDR032C; ORFNames=D3422, YD9673.02C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP IDENTIFICATION.
RX PubMed=11079560;
RX DOI=10.1002/1522-2683(20001001)21:16<3396::aid-elps3396>3.0.co;2-j;
RA Pardo M., Ward M., Bains S., Molina M., Blackstock W., Gil C., Nombela C.;
RT "A proteomic approach for the study of Saccharomyces cerevisiae cell wall
RT biogenesis.";
RL Electrophoresis 21:3396-3410(2000).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ADR376;
RX PubMed=17330950; DOI=10.1021/pr060559j;
RA Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J.,
RA Elias J.E., Gygi S.P.;
RT "Large-scale phosphorylation analysis of alpha-factor-arrested
RT Saccharomyces cerevisiae.";
RL J. Proteome Res. 6:1190-1197(2007).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19779198; DOI=10.1126/science.1172867;
RA Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT into evolution.";
RL Science 325:1682-1686(2009).
CC -!- INTERACTION:
CC Q12335; Q12335: PST2; NbExp=4; IntAct=EBI-14064, EBI-14064;
CC Q12335; P38234: RFS1; NbExp=4; IntAct=EBI-14064, EBI-21445;
CC Q12335; P25349: YCP4; NbExp=6; IntAct=EBI-14064, EBI-21840;
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- MISCELLANEOUS: Present with 2330 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the WrbA family. {ECO:0000305}.
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DR EMBL; Z74328; CAA98854.1; -; Genomic_DNA.
DR EMBL; Z68196; CAA92369.1; -; Genomic_DNA.
DR EMBL; AY557646; AAS55972.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA11876.1; -; Genomic_DNA.
DR PIR; S61585; S61585.
DR RefSeq; NP_010315.1; NM_001180340.1.
DR PDB; 5MP4; X-ray; 2.79 A; A/B/C/D/E/F/G/H=1-198.
DR PDBsum; 5MP4; -.
DR AlphaFoldDB; Q12335; -.
DR SMR; Q12335; -.
DR BioGRID; 32081; 140.
DR DIP; DIP-1762N; -.
DR IntAct; Q12335; 48.
DR MINT; Q12335; -.
DR STRING; 4932.YDR032C; -.
DR CAZy; AA6; Auxiliary Activities 6.
DR iPTMnet; Q12335; -.
DR MaxQB; Q12335; -.
DR PaxDb; Q12335; -.
DR PRIDE; Q12335; -.
DR TopDownProteomics; Q12335; -.
DR EnsemblFungi; YDR032C_mRNA; YDR032C; YDR032C.
DR GeneID; 851596; -.
DR KEGG; sce:YDR032C; -.
DR SGD; S000002439; PST2.
DR VEuPathDB; FungiDB:YDR032C; -.
DR eggNOG; KOG3135; Eukaryota.
DR GeneTree; ENSGT00940000176381; -.
DR HOGENOM; CLU_051402_0_1_1; -.
DR InParanoid; Q12335; -.
DR OMA; HGMLIMG; -.
DR BioCyc; YEAST:G3O-29647-MON; -.
DR PRO; PR:Q12335; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q12335; protein.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0005886; C:plasma membrane; HDA:SGD.
DR GO; GO:0010181; F:FMN binding; IEA:InterPro.
DR GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR GO; GO:0003955; F:NAD(P)H dehydrogenase (quinone) activity; IDA:SGD.
DR Gene3D; 3.40.50.360; -; 1.
DR InterPro; IPR008254; Flavodoxin/NO_synth.
DR InterPro; IPR029039; Flavoprotein-like_sf.
DR InterPro; IPR010089; Flavoprotein_WrbA-like.
DR InterPro; IPR005025; FMN_Rdtase-like.
DR Pfam; PF03358; FMN_red; 1.
DR SUPFAM; SSF52218; SSF52218; 1.
DR TIGRFAMs; TIGR01755; flav_wrbA; 1.
DR PROSITE; PS50902; FLAVODOXIN_LIKE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; Secreted; Signal.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..198
FT /note="Protoplast secreted protein 2"
FT /id="PRO_0000041482"
FT DOMAIN 22..191
FT /note="Flavodoxin-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00088"
FT STRAND 3..8
FT /evidence="ECO:0007829|PDB:5MP4"
FT STRAND 11..13
FT /evidence="ECO:0007829|PDB:5MP4"
FT HELIX 14..28
FT /evidence="ECO:0007829|PDB:5MP4"
FT STRAND 32..38
FT /evidence="ECO:0007829|PDB:5MP4"
FT HELIX 44..50
FT /evidence="ECO:0007829|PDB:5MP4"
FT HELIX 63..68
FT /evidence="ECO:0007829|PDB:5MP4"
FT STRAND 70..77
FT /evidence="ECO:0007829|PDB:5MP4"
FT STRAND 79..82
FT /evidence="ECO:0007829|PDB:5MP4"
FT HELIX 85..92
FT /evidence="ECO:0007829|PDB:5MP4"
FT HELIX 95..99
FT /evidence="ECO:0007829|PDB:5MP4"
FT TURN 100..105
FT /evidence="ECO:0007829|PDB:5MP4"
FT STRAND 107..117
FT /evidence="ECO:0007829|PDB:5MP4"
FT TURN 118..121
FT /evidence="ECO:0007829|PDB:5MP4"
FT HELIX 122..125
FT /evidence="ECO:0007829|PDB:5MP4"
FT HELIX 128..134
FT /evidence="ECO:0007829|PDB:5MP4"
FT HELIX 144..146
FT /evidence="ECO:0007829|PDB:5MP4"
FT HELIX 147..150
FT /evidence="ECO:0007829|PDB:5MP4"
FT STRAND 163..167
FT /evidence="ECO:0007829|PDB:5MP4"
FT STRAND 170..172
FT /evidence="ECO:0007829|PDB:5MP4"
FT HELIX 178..197
FT /evidence="ECO:0007829|PDB:5MP4"
SQ SEQUENCE 198 AA; 20966 MW; 1056E7A1F3E82288 CRC64;
MPRVAIIIYT LYGHVAATAE AEKKGIEAAG GSADIYQVEE TLSPEVVKAL GGAPKPDYPI
ATQDTLTEYD AFLFGIPTRF GNFPAQWKAF WDRTGGLWAK GALHGKVAGC FVSTGTGGGN
EATIMNSLST LAHHGIIFVP LGYKNVFAEL TNMDEVHGGS PWGAGTIAGS DGSRSPSALE
LQVHEIQGKT FYETVAKF
