ID   PSTA2_MYCBO             Reviewed;         301 AA.
AC   P0A627; A0A1R3XWW2; O08115; Q50796; X2BGM5;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2005, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Phosphate transport system permease protein PstA 2;
GN   Name=pstA2; OrderedLocusNames=BQ2027_MB0961;
OS   Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=233413;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=12788972; DOI=10.1073/pnas.1130426100;
RA   Garnier T., Eiglmeier K., Camus J.-C., Medina N., Mansoor H., Pryor M.,
RA   Duthoy S., Grondin S., Lacroix C., Monsempe C., Simon S., Harris B.,
RA   Atkin R., Doggett J., Mayes R., Keating L., Wheeler P.R., Parkhill J.,
RA   Barrell B.G., Cole S.T., Gordon S.V., Hewinson R.G.;
RT   "The complete genome sequence of Mycobacterium bovis.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:7877-7882(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-935 / AF2122/97;
RX   PubMed=28385856; DOI=10.1128/genomea.00157-17;
RA   Malone K.M., Farrell D., Stuber T.P., Schubert O.T., Aebersold R.,
RA   Robbe-Austerman S., Gordon S.V.;
RT   "Updated reference genome sequence and annotation of Mycobacterium bovis
RT   AF2122/97.";
RL   Genome Announc. 5:E00157-E00157(2017).
CC   -!- FUNCTION: Part of the binding-protein-dependent transport system for
CC       phosphate; probably responsible for the translocation of the substrate
CC       across the membrane. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the binding-protein-dependent transport system
CC       permease family. CysTW subfamily. {ECO:0000305}.
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DR   EMBL; LT708304; SIT99559.1; -; Genomic_DNA.
DR   RefSeq; NP_854618.1; NC_002945.3.
DR   RefSeq; WP_003404794.1; NC_002945.4.
DR   AlphaFoldDB; P0A627; -.
DR   SMR; P0A627; -.
DR   TCDB; 3.A.1.7.4; the atp-binding cassette (abc) superfamily.
DR   PRIDE; P0A627; -.
DR   EnsemblBacteria; SIT99559; SIT99559; BQ2027_MB0961.
DR   PATRIC; fig|233413.5.peg.1046; -.
DR   OMA; YSNWNPF; -.
DR   Proteomes; UP000001419; Chromosome.
DR   GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR   GO; GO:0005315; F:inorganic phosphate transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0035435; P:phosphate ion transmembrane transport; IEA:InterPro.
DR   CDD; cd06261; TM_PBP2; 1.
DR   Gene3D; 1.10.3720.10; -; 1.
DR   InterPro; IPR000515; MetI-like.
DR   InterPro; IPR035906; MetI-like_sf.
DR   InterPro; IPR005672; Phosphate_PstA.
DR   Pfam; PF00528; BPD_transp_1; 1.
DR   SUPFAM; SSF161098; SSF161098; 1.
DR   TIGRFAMs; TIGR00974; 3a0107s02c; 1.
DR   PROSITE; PS50928; ABC_TM1; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Membrane; Phosphate transport; Transmembrane;
KW   Transmembrane helix; Transport.
FT   CHAIN           1..301
FT                   /note="Phosphate transport system permease protein PstA 2"
FT                   /id="PRO_0000060202"
FT   TRANSMEM        36..56
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        83..103
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        127..147
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        149..169
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        209..229
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   TRANSMEM        274..294
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
FT   DOMAIN          83..288
FT                   /note="ABC transmembrane type-1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00441"
SQ   SEQUENCE   301 AA;  32236 MW;  1B602BBD39733C40 CRC64;
     MGESAESGSR QLPAMSPPRR SVAYRRKIVD ALWWAACVCC LAVVITPTLW MLIGVVSRAV
     PVFHWSVLVQ DSQGNGGGLR NAIIGTAVLA IGVILVGGTV SVLTGIYLSE FATGKTRSIL
     RGAYEVLSGI PSIVLGYVGY LALVVYFDWG FSLAAGVLVL SVMSIPYIAK ATESALAQVP
     TSYREAAEAL GLPAGWALRK IVLKTAMPGI VTGMLVALAL AIGETAPLLY TAGWSNSPPT
     GQLTDSPVGY LTYPIWTFYN QPSKSAQDLS YDAALLLIVF LLLLIFIGRL INWLSRRRWD
     V