PSTA_DICDI
ID PSTA_DICDI Reviewed; 1053 AA.
AC P11976; Q55BP6;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 2.
DT 25-MAY-2022, entry version 113.
DE RecName: Full=Prestalk protein;
DE AltName: Full=Extracellular matrix protein ST310;
DE Flags: Precursor;
GN Name=ecmB; ORFNames=DDB_G0269132;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=AX2;
RX PubMed=3658700; DOI=10.1093/nar/15.18.7463;
RA Ceccarelli A., McRobbie S.J., Jermyn K.A., Duffy K., Early A.,
RA Williams J.G.;
RT "Structural and functional characterization of a Dictyostelium gene
RT encoding a DIF inducible, prestalk-enriched mRNA sequence.";
RL Nucleic Acids Res. 15:7463-7476(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-17.
RX PubMed=3600646; DOI=10.1128/mcb.7.5.1823-1829.1987;
RA Ayres K., Neuman W., Rowekamp W.G., Chung S.;
RT "Developmental regulation of DNase I-hypersensitive sites in Dictyostelium
RT discoideum.";
RL Mol. Cell. Biol. 7:1823-1829(1987).
CC -!- FUNCTION: Component of the stalk tube, the matrix that encases stalk
CC cells.
CC -!- SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular
CC matrix.
CC -!- INDUCTION: By the putative stalk-specific morphogen DIF
CC (differentiation inducing factor).
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DR EMBL; AAFI02000005; EAL71916.1; -; Genomic_DNA.
DR EMBL; M16345; AAA33216.1; -; Genomic_DNA.
DR PIR; A26838; A26838.
DR RefSeq; XP_646785.1; XM_641693.1.
DR AlphaFoldDB; P11976; -.
DR STRING; 44689.DDB0216219; -.
DR PaxDb; P11976; -.
DR PRIDE; P11976; -.
DR EnsemblProtists; EAL71916; EAL71916; DDB_G0269132.
DR GeneID; 8617758; -.
DR KEGG; ddi:DDB_G0269132; -.
DR dictyBase; DDB_G0269132; ecmB.
DR eggNOG; ENOG502RSRD; Eukaryota.
DR HOGENOM; CLU_290611_0_0_1; -.
DR InParanoid; P11976; -.
DR OMA; CTEDKCT; -.
DR PhylomeDB; P11976; -.
DR PRO; PR:P11976; -.
DR Proteomes; UP000002195; Chromosome 1.
DR GO; GO:0031012; C:extracellular matrix; TAS:dictyBase.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0005198; F:structural molecule activity; TAS:dictyBase.
DR GO; GO:0031154; P:culmination involved in sorocarp development; IEP:dictyBase.
DR GO; GO:0030198; P:extracellular matrix organization; TAS:dictyBase.
DR InterPro; IPR001673; S_mold_repeat.
DR Pfam; PF00526; Dicty_CTDC; 41.
PE 2: Evidence at transcript level;
KW Extracellular matrix; Reference proteome; Repeat; Secreted; Signal.
FT SIGNAL 1..18
FT /evidence="ECO:0000305"
FT CHAIN 19..1053
FT /note="Prestalk protein"
FT /id="PRO_0000022171"
FT REPEAT 38..60
FT /note="X-1"
FT REPEAT 61..84
FT /note="X-2"
FT REPEAT 85..108
FT /note="X-3"
FT REPEAT 109..132
FT /note="A-1"
FT REPEAT 133..156
FT /note="A-2"
FT REPEAT 157..180
FT /note="B-1"
FT REPEAT 181..204
FT /note="A-3"
FT REPEAT 205..228
FT /note="A-4"
FT REPEAT 229..252
FT /note="B-2"
FT REPEAT 253..276
FT /note="A-5"
FT REPEAT 277..300
FT /note="A-6"
FT REPEAT 301..324
FT /note="B-3"
FT REPEAT 325..348
FT /note="A-7"
FT REPEAT 349..372
FT /note="A-8"
FT REPEAT 373..396
FT /note="B-4"
FT REPEAT 397..420
FT /note="A-9"
FT REPEAT 421..444
FT /note="A-10"
FT REPEAT 445..468
FT /note="B-5"
FT REPEAT 469..492
FT /note="A-11"
FT REPEAT 493..516
FT /note="A-12"
FT REPEAT 517..540
FT /note="B-6"
FT REPEAT 541..564
FT /note="A-13"
FT REPEAT 565..588
FT /note="X-4"
FT REPEAT 589..612
FT /note="A-14"
FT REPEAT 613..636
FT /note="A-15"
FT REPEAT 637..660
FT /note="A-16"
FT REPEAT 661..684
FT /note="B-7"
FT REPEAT 685..708
FT /note="A-17"
FT REPEAT 709..732
FT /note="A-18"
FT REPEAT 733..756
FT /note="B-8"
FT REPEAT 757..780
FT /note="A-19"
FT REPEAT 781..804
FT /note="A-20"
FT REPEAT 805..828
FT /note="B-9"
FT REPEAT 829..852
FT /note="A-21"
FT REPEAT 853..876
FT /note="A-22"
FT REPEAT 877..900
FT /note="A-23"
FT REPEAT 901..924
FT /note="B-10"
FT REPEAT 925..948
FT /note="A-24"
FT REPEAT 949..971
FT /note="A-25"
FT REPEAT 972..995
FT /note="A-26"
FT REPEAT 996..1019
FT /note="A-27"
FT REGION 38..1019
FT /note="41 X 24 AA tandem repeats, Cys-rich"
FT CONFLICT 19..25
FT /note="Missing (in Ref. 1; AAA33216)"
FT /evidence="ECO:0000305"
FT CONFLICT 87
FT /note="C -> Y (in Ref. 1; AAA33216)"
FT /evidence="ECO:0000305"
FT CONFLICT 154..155
FT /note="DD -> VV (in Ref. 1; AAA33216)"
FT /evidence="ECO:0000305"
FT CONFLICT 165..169
FT /note="TQSGG -> IQLGV (in Ref. 1; AAA33216)"
FT /evidence="ECO:0000305"
FT CONFLICT 206
FT /note="A -> P (in Ref. 1; AAA33216)"
FT /evidence="ECO:0000305"
FT CONFLICT 266
FT /note="C -> S (in Ref. 1; AAA33216)"
FT /evidence="ECO:0000305"
FT CONFLICT 344
FT /note="A -> P (in Ref. 1; AAA33216)"
FT /evidence="ECO:0000305"
FT CONFLICT 415..416
FT /note="MS -> IR (in Ref. 1; AAA33216)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1053 AA; 109370 MW; EA523D41B0438363 CRC64;
MNKIYLILIL FTFVGIILAN VEKAEVSCTC DENCNDGNKC TLDKCNNGCC SNTPININDN
DECTVDTCNP KTGISHTPVN CDDGNSCTAD SCLCGKGCQH VPIACDDNNA CTVDSCSNST
GCCHTPLSCD DNNPCTVDSC SNSTGCCHTP INVDDHNACT EDKCTQSGGV THTPIACDDK
NACTVDSCSN STGCCHTPLS CDDNNACTVD SCSNSTGCVH TPINVDDHNA CTEDKCTQSG
GVTHTPIACD DKNACTADSC SNSTGCCHTP ITCDDNNACT VDSCSNSTGC CHTPINVDDN
NACTEDKCTQ SGGVTHTPIA CDDKNACTVD SCSNSTGCVH TPLACDDKNP CTVDSCSNST
GCCHTPINVD DNNACTEDKC TQSGGVTHTP INCDDNNKCT VDSCSNSTGC CHTPMSCDDN
NPCTVDSCSN STGCVHTPIN VDDNNACTED KCTQNGGVTH TPIACDDKNA CTVDSCSNST
GCCHTPLKCD DNNACTVDSC SNSTGCVHTP INVDDNNACT EDKCTQSGGV THTPISCDDK
NPCTIDSCSN STGCVHTPMS CDDRNPCTSD FCSWEKGCQH VALSCNDFNA CTMDSCSNST
GCTHTPIACD DKNACTVDSC SNSTGCVHTP LTCDDNNPCT VDSCSNSTGC CHTPINVDDH
NACTEDKCTQ SGGVTHTPIA CDDKNACTVD SCSNSTGCCH TPLSCDDNNA CTVDSCSNST
GCVHTPINVD DNNACTEDKC TQNGGVTHTP IACDDKNACT VDSCSNSTGC CHTPLKCDDN
NPCTVDSCSN STGCVHTPMN VDDNNACTED KCTQNGGVTH TPIRCDDLNS CTADSCSNST
GCVHTPINCD DNNKCTADSC SNSTGCCHTP ISCDDNNPCT VDSCSNSTGC CHTPINVDDN
NPCTEDKCTQ SGGVTHTPIG CNDNNACTVD SCSNSTGCTH TPMKCDDNNP CTIDSCSNST
GCVHTPMNCD DGNFCTLDSC CSTGCTHTPI IIDDNNPCTV DSCCNSTGVV HTPVDCNDNN
VLTCDYCSIK QGGKCIHVPM TQCKTFGKCG DDL
