ATO3_YEAST
ID ATO3_YEAST Reviewed; 275 AA.
AC Q12359; D6VT18;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Ammonia transport outward protein 3;
GN Name=ATO3; OrderedLocusNames=YDR384C; ORFNames=D9481.20, D9509.4;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION.
RX PubMed=12429834; DOI=10.1091/mbc.e01-12-0149;
RA Palkova Z., Devaux F., Icicova M., Minarikova L., Le Crom S., Jacq C.;
RT "Ammonia pulses and metabolic oscillations guide yeast colony
RT development.";
RL Mol. Biol. Cell 13:3901-3914(2002).
RN [4]
RP INDUCTION, AND SUBCELLULAR LOCATION.
RX PubMed=12966084; DOI=10.1074/jbc.m309301200;
RA Guaragnella N., Butow R.A.;
RT "ATO3 encoding a putative outward ammonium transporter is an RTG-
RT independent retrograde responsive gene regulated by GCN4 and the Ssy1-Ptr3-
RT Ssy5 amino acid sensor system.";
RL J. Biol. Chem. 278:45882-45887(2003).
RN [5]
RP INDUCTION.
RX PubMed=15743812; DOI=10.1128/mcb.25.6.2138-2146.2005;
RA Tachibana C., Yoo J.Y., Tagne J.-B., Kacherovsky N., Lee T.I., Young E.T.;
RT "Combined global localization analysis and transcriptome data identify
RT genes that are directly coregulated by Adr1 and Cat8.";
RL Mol. Cell. Biol. 25:2138-2146(2005).
RN [6]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN [7]
RP SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=17395151; DOI=10.1016/j.bbamem.2007.02.011;
RA Ricicova M., Kucerova H., Vachova L., Palkova Z.;
RT "Association of putative ammonium exporters Ato with detergent-resistant
RT compartments of plasma membrane during yeast colony development: pH affects
RT Ato1p localisation in patches.";
RL Biochim. Biophys. Acta 1768:1170-1178(2007).
RN [8]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-4, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 76625 / YPH499;
RX PubMed=17761666; DOI=10.1074/mcp.m700098-mcp200;
RA Reinders J., Wagner K., Zahedi R.P., Stojanovski D., Eyrich B.,
RA van der Laan M., Rehling P., Sickmann A., Pfanner N., Meisinger C.;
RT "Profiling phosphoproteins of yeast mitochondria reveals a role of
RT phosphorylation in assembly of the ATP synthase.";
RL Mol. Cell. Proteomics 6:1896-1906(2007).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
CC -!- FUNCTION: Transporter protein required for ammonia export. Induced in
CC rho(0) cells, probably to eliminate the excess ammonia that arises
CC because of a potential defect in ammonia assimilation in those cells.
CC {ECO:0000269|PubMed:12429834}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:12966084,
CC ECO:0000269|PubMed:17395151}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:12966084, ECO:0000269|PubMed:17395151}.
CC Note=Localizes to large detergent resistant patches of the cell
CC membrane (DRM) enriched in ergosterol and sphingolipids.
CC -!- INDUCTION: Expression is under the control of the ADR1, CAT8, and GCN4
CC transcription factors. Also induced by external ammonia and in rho(0)
CC cells. {ECO:0000269|PubMed:12966084, ECO:0000269|PubMed:15743812,
CC ECO:0000269|PubMed:17395151}.
CC -!- SIMILARITY: Belongs to the acetate uptake transporter (AceTr) (TC
CC 2.A.96) family. {ECO:0000305}.
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DR EMBL; U28373; AAB64820.1; -; Genomic_DNA.
DR EMBL; U32274; AAB64826.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA12228.1; -; Genomic_DNA.
DR PIR; S61179; S61179.
DR RefSeq; NP_010672.1; NM_001180692.1.
DR AlphaFoldDB; Q12359; -.
DR SMR; Q12359; -.
DR BioGRID; 32445; 23.
DR DIP; DIP-4513N; -.
DR IntAct; Q12359; 2.
DR MINT; Q12359; -.
DR STRING; 4932.YDR384C; -.
DR TCDB; 2.A.96.1.5; the acetate uptake transporter (acetr) family.
DR iPTMnet; Q12359; -.
DR PaxDb; Q12359; -.
DR PRIDE; Q12359; -.
DR TopDownProteomics; Q12359; -.
DR EnsemblFungi; YDR384C_mRNA; YDR384C; YDR384C.
DR GeneID; 851992; -.
DR KEGG; sce:YDR384C; -.
DR SGD; S000002792; ATO3.
DR VEuPathDB; FungiDB:YDR384C; -.
DR eggNOG; ENOG502QSVX; Eukaryota.
DR GeneTree; ENSGT00940000176398; -.
DR HOGENOM; CLU_051062_0_0_1; -.
DR InParanoid; Q12359; -.
DR OMA; CINTDQF; -.
DR BioCyc; YEAST:G3O-29932-MON; -.
DR PRO; PR:Q12359; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q12359; protein.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR GO; GO:0015123; F:acetate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0008519; F:ammonium transmembrane transporter activity; IMP:SGD.
DR GO; GO:0072488; P:ammonium transmembrane transport; IMP:SGD.
DR GO; GO:0019740; P:nitrogen utilization; IMP:SGD.
DR GO; GO:0055085; P:transmembrane transport; IMP:SGD.
DR InterPro; IPR000791; Gpr1/Fun34/SatP.
DR Pfam; PF01184; Gpr1_Fun34_YaaH; 1.
DR PROSITE; PS01114; GPR1_FUN34_YAAH; 1.
PE 1: Evidence at protein level;
KW Ammonia transport; Cell membrane; Ion transport; Membrane; Phosphoprotein;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..275
FT /note="Ammonia transport outward protein 3"
FT /id="PRO_0000135707"
FT TOPO_DOM 1..84
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 85..105
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 106..120
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 121..141
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 142..181
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 182..202
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 203..207
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 208..228
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 229..236
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 237..257
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 258..275
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT MOD_RES 4
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:17761666"
SQ SEQUENCE 275 AA; 30028 MW; CB4E91128403C6CC CRC64;
MTSSASSPQD LEKGVNTLEN IETLPQQGSI AGVSQGFPNI QEIYSDRDFI TLGSSTYRRR
DLLNALDRGD GEEGNCAKYT PHQFANPVPL GLASFSLSCL VLSLINANVR GVTDGKWALS
LFMFFGGAIE LFAGLLCFVI GDTYAMTVFS SFGGFWICYG YGLTDTDNLV SGYTDPTMLN
NVIGFFLAGW TVFTFLMLMC TLKSTWGLFL LLTFLDLTFL LLCIGTFIDN NNLKMAGGYF
GILSSCCGWY SLYCSVVSPS NSYLAFRAHT MPNAP
