ID   ATOB_PSEAE              Reviewed;         393 AA.
AC   Q9I2A8;
DT   15-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=Acetyl-CoA acetyltransferase;
DE            EC=2.3.1.9;
DE   AltName: Full=Acetoacetyl-CoA thiolase;
GN   Name=atoB; OrderedLocusNames=PA2001;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 acetyl-CoA = acetoacetyl-CoA + CoA; Xref=Rhea:RHEA:21036,
CC         ChEBI:CHEBI:57286, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.9;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10020};
CC   -!- PATHWAY: Metabolic intermediate biosynthesis; (R)-mevalonate
CC       biosynthesis; (R)-mevalonate from acetyl-CoA: step 1/3.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the thiolase-like superfamily. Thiolase family.
CC       {ECO:0000305}.
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DR   EMBL; AE004091; AAG05389.1; -; Genomic_DNA.
DR   PIR; C83396; C83396.
DR   RefSeq; NP_250691.1; NC_002516.2.
DR   RefSeq; WP_003113500.1; NZ_QZGE01000026.1.
DR   AlphaFoldDB; Q9I2A8; -.
DR   SMR; Q9I2A8; -.
DR   STRING; 287.DR97_5847; -.
DR   PaxDb; Q9I2A8; -.
DR   PRIDE; Q9I2A8; -.
DR   DNASU; 879046; -.
DR   EnsemblBacteria; AAG05389; AAG05389; PA2001.
DR   GeneID; 879046; -.
DR   KEGG; pae:PA2001; -.
DR   PATRIC; fig|208964.12.peg.2085; -.
DR   PseudoCAP; PA2001; -.
DR   HOGENOM; CLU_031026_0_0_6; -.
DR   InParanoid; Q9I2A8; -.
DR   OMA; VNQFHGA; -.
DR   PhylomeDB; Q9I2A8; -.
DR   BioCyc; PAER208964:G1FZ6-2039-MON; -.
DR   UniPathway; UPA00058; UER00101.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003985; F:acetyl-CoA C-acetyltransferase activity; IBA:GO_Central.
DR   GO; GO:0006635; P:fatty acid beta-oxidation; IBA:GO_Central.
DR   CDD; cd00751; thiolase; 1.
DR   Gene3D; 3.40.47.10; -; 2.
DR   InterPro; IPR002155; Thiolase.
DR   InterPro; IPR016039; Thiolase-like.
DR   InterPro; IPR020615; Thiolase_acyl_enz_int_AS.
DR   InterPro; IPR020610; Thiolase_AS.
DR   InterPro; IPR020617; Thiolase_C.
DR   InterPro; IPR020613; Thiolase_CS.
DR   InterPro; IPR020616; Thiolase_N.
DR   Pfam; PF02803; Thiolase_C; 1.
DR   Pfam; PF00108; Thiolase_N; 1.
DR   PIRSF; PIRSF000429; Ac-CoA_Ac_transf; 1.
DR   SUPFAM; SSF53901; SSF53901; 2.
DR   TIGRFAMs; TIGR01930; AcCoA-C-Actrans; 1.
DR   PROSITE; PS00098; THIOLASE_1; 1.
DR   PROSITE; PS00737; THIOLASE_2; 1.
DR   PROSITE; PS00099; THIOLASE_3; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Cytoplasm; Fatty acid metabolism; Lipid metabolism;
KW   Reference proteome; Transferase.
FT   CHAIN           1..393
FT                   /note="Acetyl-CoA acetyltransferase"
FT                   /id="PRO_0000287828"
FT   ACT_SITE        88
FT                   /note="Acyl-thioester intermediate"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        349
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10020"
FT   ACT_SITE        379
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10020"
SQ   SEQUENCE   393 AA;  40405 MW;  4BFFEA8F1D8352B8 CRC64;
     MQDVVIVAAT RTAVGSFQGS LAGIPAPELG AAVIRRLLEQ TGLDAGQVDE VILGQVLTAG
     SGQNPARQAA IKAGLPVGVP AMTLNKVCGS GLKALHLGAQ AIRCGDAEVI VAGGQENMSL
     APYVMPGART GLRMGHAKLV DSMIEDGLWD AFNDYHMGIT AENLAEKYGL TREEQDAFAA
     ASQQKAIAAI EGGRFRDEIT PIQVPQRKGE PLGFDTDEQP RAGTTVEALA KLKPAFRKDG
     SVTAGNASSL NDGAAAVLLM SAAKAKALGL PVLARIASYA SAGVDPAIMG IGPVSATRRA
     LDKAGWSLEQ LDLIEANEAF AAQSLAVGRE LGWDAARVNV NGGAIALGHP IGASGCRVLV
     TLLHEMIRRD AKKGLATLCI GGGQGVALTL ARD