PSTB2_MYCTU
ID PSTB2_MYCTU Reviewed; 276 AA.
AC P9WQK9; L0T5C0; O05869; P95302;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 03-AUG-2022, entry version 45.
DE RecName: Full=Phosphate import ATP-binding protein PstB 2 {ECO:0000255|HAMAP-Rule:MF_01702};
DE EC=7.3.2.1 {ECO:0000255|HAMAP-Rule:MF_01702};
DE AltName: Full=ABC phosphate transporter 2 {ECO:0000255|HAMAP-Rule:MF_01702};
DE AltName: Full=Phosphate-transporting ATPase 2 {ECO:0000255|HAMAP-Rule:MF_01702};
GN Name=pstB2 {ECO:0000255|HAMAP-Rule:MF_01702}; Synonyms=pstB;
GN OrderedLocusNames=Rv0933; ORFNames=MTCY08D9.06c;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 35801 / TMC 107 / Erdman;
RX PubMed=8918249; DOI=10.1016/0378-1119(96)00242-9;
RA Braibant M., Lefevre P., de Wit L., Peirs P., Ooms J., Huygen K.,
RA Andersen A.B., Content J.;
RT "A Mycobacterium tuberculosis gene cluster encoding proteins of a phosphate
RT transporter homologous to the Escherichia coli Pst system.";
RL Gene 176:171-176(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [3]
RP FUNCTION, AND INDUCTION.
RC STRAIN=H37Rv;
RX PubMed=20933472; DOI=10.1016/j.tube.2010.09.004;
RA Vanzembergh F., Peirs P., Lefevre P., Celio N., Mathys V., Content J.,
RA Kalai M.;
RT "Effect of PstS sub-units or PknD deficiency on the survival of
RT Mycobacterium tuberculosis.";
RL Tuberculosis 90:338-345(2010).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC -!- FUNCTION: Part of the ABC transporter complex PstSACB involved in
CC phosphate import (Probable). Responsible for energy coupling to the
CC transport system. {ECO:0000255|HAMAP-Rule:MF_01702,
CC ECO:0000305|PubMed:20933472}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + phosphate(out) = ADP + H(+) + 2 phosphate(in);
CC Xref=Rhea:RHEA:24440, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=7.3.2.1;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01702};
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (PstB),
CC two transmembrane proteins (PstC and PstA) and a solute-binding protein
CC (PstS). {ECO:0000255|HAMAP-Rule:MF_01702}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01702};
CC Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_01702}.
CC -!- INDUCTION: Slightly induced by phosphate starvation, part of the pstB3-
CC pstS2-pstC1-pstA2 operon. {ECO:0000269|PubMed:20933472}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Phosphate
CC importer (TC 3.A.1.7) family. {ECO:0000255|HAMAP-Rule:MF_01702}.
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DR EMBL; Z47981; CAA88024.1; -; Genomic_DNA.
DR EMBL; AL123456; CCP43681.1; -; Genomic_DNA.
DR PIR; E70584; E70584.
DR RefSeq; NP_215448.1; NC_000962.3.
DR RefSeq; WP_003898653.1; NZ_NVQJ01000001.1.
DR AlphaFoldDB; P9WQK9; -.
DR SMR; P9WQK9; -.
DR STRING; 83332.Rv0933; -.
DR PaxDb; P9WQK9; -.
DR DNASU; 885653; -.
DR GeneID; 45424899; -.
DR GeneID; 885653; -.
DR KEGG; mtu:Rv0933; -.
DR TubercuList; Rv0933; -.
DR eggNOG; COG1117; Bacteria.
DR OMA; NLFPMSV; -.
DR PhylomeDB; P9WQK9; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR GO; GO:0005524; F:ATP binding; IDA:MTBBASE.
DR GO; GO:0016887; F:ATP hydrolysis activity; IDA:MTBBASE.
DR GO; GO:0015415; F:ATPase-coupled phosphate ion transmembrane transporter activity; IEA:UniProtKB-EC.
DR GO; GO:0005315; F:inorganic phosphate transmembrane transporter activity; IEA:InterPro.
DR CDD; cd03260; ABC_PstB_phosphate_transporter; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005670; Phosp_transpt1.
DR PANTHER; PTHR43423; PTHR43423; 1.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00972; 3a0107s01c2; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR PROSITE; PS51238; PSTB; 1.
PE 1: Evidence at protein level;
KW ATP-binding; Cell membrane; Membrane; Nucleotide-binding;
KW Phosphate transport; Reference proteome; Translocase; Transport.
FT CHAIN 1..276
FT /note="Phosphate import ATP-binding protein PstB 2"
FT /id="PRO_0000092850"
FT DOMAIN 22..262
FT /note="ABC transporter"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01702"
FT BINDING 54..61
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01702"
FT CONFLICT 124..136
FT /note="GVRAHKLVPRKEF -> ACVPTNWCRARNS (in Ref. 1;
FT CAA88024)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 276 AA; 29996 MW; F52045DD1EF9580B CRC64;
MACERLGGQS GAADVDAAAP AMAAVNLTLG FAGKTVLDQV SMGFPARAVT SLMGPTGSGK
TTFLRTLNRM NDKVSGYRYS GDVLLGGRSI FNYRDVLEFR RRVGMLFQRP NPFPMSIMDN
VLAGVRAHKL VPRKEFRGVA QARLTEVGLW DAVKDRLSDS PFRLSGGQQQ LLCLARTLAV
NPEVLLLDEP TSALDPTTTE KIEEFIRSLA DRLTVIIVTH NLAQAARISD RAALFFDGRL
VEEGPTEQLF SSPKHAETAR YVAGLSGDVK DAKRGN
