ID   ATOE_ECOLI              Reviewed;         440 AA.
AC   P76460;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 141.
DE   RecName: Full=Putative short-chain fatty acid transporter;
GN   Name=atoE; OrderedLocusNames=b2223, JW2217;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA   Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA   Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA   Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA   Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA   Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA   Horiuchi T.;
RT   "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 40.1-50.0 min region on the linkage map.";
RL   DNA Res. 3:379-392(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=15919996; DOI=10.1126/science.1109730;
RA   Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT   "Global topology analysis of the Escherichia coli inner membrane
RT   proteome.";
RL   Science 308:1321-1323(2005).
CC   -!- FUNCTION: May be responsible for the uptake of short-chain fatty acids.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
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DR   EMBL; U00096; AAC75283.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA16019.1; -; Genomic_DNA.
DR   PIR; E64992; E64992.
DR   RefSeq; NP_416727.1; NC_000913.3.
DR   RefSeq; WP_000580275.1; NZ_STEB01000002.1.
DR   AlphaFoldDB; P76460; -.
DR   BioGRID; 4261814; 7.
DR   STRING; 511145.b2223; -.
DR   TCDB; 2.A.73.1.1; the short chain fatty acid uptake (atoe) family.
DR   PaxDb; P76460; -.
DR   PRIDE; P76460; -.
DR   EnsemblBacteria; AAC75283; AAC75283; b2223.
DR   EnsemblBacteria; BAA16019; BAA16019; BAA16019.
DR   GeneID; 946721; -.
DR   KEGG; ecj:JW2217; -.
DR   KEGG; eco:b2223; -.
DR   PATRIC; fig|1411691.4.peg.12; -.
DR   EchoBASE; EB1622; -.
DR   eggNOG; COG2031; Bacteria.
DR   HOGENOM; CLU_037744_0_0_6; -.
DR   InParanoid; P76460; -.
DR   OMA; QDKTIAH; -.
DR   PhylomeDB; P76460; -.
DR   BioCyc; EcoCyc:EG11671-MON; -.
DR   BioCyc; MetaCyc:EG11671-MON; -.
DR   PRO; PR:P76460; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR   InterPro; IPR006161; CHP00366.
DR   InterPro; IPR006160; SCFA_transpt_AtoE.
DR   PANTHER; PTHR41983; PTHR41983; 1.
DR   Pfam; PF02667; SCFA_trans; 1.
DR   TIGRFAMs; TIGR00366; TIGR00366; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..440
FT                   /note="Putative short-chain fatty acid transporter"
FT                   /id="PRO_0000064733"
FT   TOPO_DOM        1..19
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        20..40
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        41..53
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        54..74
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        75..102
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        103..123
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        124..137
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        138..158
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        159..179
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        180
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        181..201
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        202..244
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        245..265
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        266..286
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        287..313
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        314..334
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        335..351
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        352..372
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        373..394
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        395..415
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        416..419
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        420..440
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   440 AA;  47528 MW;  5C9D5ECE79F5FA71 CRC64;
     MIGRISRFMT RFVSRWLPDP LIFAMLLTLL TFVIALWLTP QTPISMVKMW GDGFWNLLAF
     GMQMALIIVT GHALASSAPV KSLLRTAASA AKTPVQGVML VTFFGSVACV INWGFGLVVG
     AMFAREVARR VPGSDYPLLI ACAYIGFLTW GGGFSGSMPL LAATPGNPVE HIAGLIPVGD
     TLFSGFNIFI TVALIVVMPF ITRMMMPKPS DVVSIDPKLL MEEADFQKQL PKDAPPSERL
     EESRILTLII GALGIAYLAM YFSEHGFNIT INTVNLMFMI AGLLLHKTPM AYMRAISAAA
     RSTAGILVQF PFYAGIQLMM EHSGLGGLIT EFFINVANKD TFPVMTFFSS ALINFAVPSG
     GGHWVIQGPF VIPAAQALGA DLGKSVMAIA YGEQWMNMAQ PFWALPALAI AGLGVRDIMG
     YCITALLFSG VIFVIGLTLF