PSTB_EDWTA
ID PSTB_EDWTA Reviewed; 259 AA.
AC Q9AML4; Q79RI8; Q8KY87;
DT 23-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Phosphate import ATP-binding protein PstB {ECO:0000255|HAMAP-Rule:MF_01702};
DE EC=7.3.2.1 {ECO:0000255|HAMAP-Rule:MF_01702};
DE AltName: Full=ABC phosphate transporter {ECO:0000255|HAMAP-Rule:MF_01702};
DE AltName: Full=Peripheral membrane protein B;
DE AltName: Full=Phosphate-transporting ATPase {ECO:0000255|HAMAP-Rule:MF_01702};
GN Name=pstB {ECO:0000255|HAMAP-Rule:MF_01702};
OS Edwardsiella tarda.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Hafniaceae; Edwardsiella.
OX NCBI_TaxID=636;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Tan Y.P., Leung K.Y.;
RL Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PPD130/91;
RX PubMed=11158362; DOI=10.1099/00221287-147-2-449;
RA Mathew J.A., Tan Y.P., Srinivasa Rao P.S., Lim T.M., Leung K.Y.;
RT "Edwardsiella tarda mutants defective in siderophore production, motility,
RT serum resistance and catalase activity.";
RL Microbiology 147:449-457(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=PPD130/91;
RX PubMed=12595451; DOI=10.1128/iai.71.3.1343-1351.2003;
RA Srinivasa Rao P.S., Lim T.M., Leung K.Y.;
RT "Functional genomics approach to the identification of virulence genes
RT involved in Edwardsiella tarda pathogenesis.";
RL Infect. Immun. 71:1343-1351(2003).
CC -!- FUNCTION: Part of the ABC transporter complex PstSACB involved in
CC phosphate import. Responsible for energy coupling to the transport
CC system.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + phosphate(out) = ADP + H(+) + 2 phosphate(in);
CC Xref=Rhea:RHEA:24440, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=7.3.2.1;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01702};
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (PstB),
CC two transmembrane proteins (PstC and PstA) and a solute-binding protein
CC (PstS). {ECO:0000255|HAMAP-Rule:MF_01702}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000255|HAMAP-
CC Rule:MF_01702}; Peripheral membrane protein {ECO:0000255|HAMAP-
CC Rule:MF_01702}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Phosphate
CC importer (TC 3.A.1.7) family. {ECO:0000255|HAMAP-Rule:MF_01702}.
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DR EMBL; AF248495; AAM45396.1; -; Genomic_DNA.
DR EMBL; AF324340; AAK12106.1; -; Genomic_DNA.
DR EMBL; AY090560; AAN05784.1; -; Genomic_DNA.
DR RefSeq; WP_015462590.1; NZ_JRGQ01000066.1.
DR AlphaFoldDB; Q9AML4; -.
DR SMR; Q9AML4; -.
DR STRING; 667121.ET1_13_02020; -.
DR GeneID; 58257739; -.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0015415; F:ATPase-coupled phosphate ion transmembrane transporter activity; IEA:UniProtKB-EC.
DR GO; GO:0005315; F:inorganic phosphate transmembrane transporter activity; IEA:InterPro.
DR CDD; cd03260; ABC_PstB_phosphate_transporter; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR015850; ABC_transpr_PstB.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005670; Phosp_transpt1.
DR PANTHER; PTHR43423; PTHR43423; 1.
DR PANTHER; PTHR43423:SF3; PTHR43423:SF3; 1.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00972; 3a0107s01c2; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR PROSITE; PS51238; PSTB; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell inner membrane; Cell membrane; Membrane;
KW Nucleotide-binding; Phosphate transport; Translocase; Transport.
FT CHAIN 1..259
FT /note="Phosphate import ATP-binding protein PstB"
FT /id="PRO_0000092813"
FT DOMAIN 13..254
FT /note="ABC transporter"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01702"
FT BINDING 45..52
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01702"
FT CONFLICT 8
FT /note="P -> T (in Ref. 1; AAM45396)"
FT /evidence="ECO:0000305"
FT CONFLICT 37
FT /note="K -> Q (in Ref. 1; AAM45396)"
FT /evidence="ECO:0000305"
FT CONFLICT 143
FT /note="S -> N (in Ref. 1; AAM45396)"
FT /evidence="ECO:0000305"
FT CONFLICT 171
FT /note="A -> G (in Ref. 1; AAM45396)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 259 AA; 29284 MW; 5C465E0A3478C2BD CRC64;
MSNVVTDPTN SKIQVRDLNF YYGKFHALKN ISLDIEKNKV TAFIGPSGCG KSTLLRTFNK
MFQLYPEQRA EGEILLDGQN ILTDSQDVAL LRAKVGMVFQ KPTPFPMSIY DNIAFGVRLF
EKLSRADMDE RVQWALTKAA LWSETKDKLH QSGYSLSGGQ QQRLCIARGI AIRPEVLLLD
EPCSALDPIS TSRIEELITE LKQDYTVVIV THNMQQAARC SDYTAFMYLG ELIEFSDTDK
LFTAPAQRQT EDYITGRYG
