PSTB_MYCS2
ID PSTB_MYCS2 Reviewed; 258 AA.
AC A0R4C0; I7GET6; O68469; Q7WTY5;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 03-AUG-2022, entry version 101.
DE RecName: Full=Phosphate import ATP-binding protein PstB {ECO:0000255|HAMAP-Rule:MF_01702};
DE EC=7.3.2.1 {ECO:0000255|HAMAP-Rule:MF_01702};
DE AltName: Full=ABC phosphate transporter {ECO:0000255|HAMAP-Rule:MF_01702};
DE AltName: Full=Phosphate-transporting ATPase {ECO:0000255|HAMAP-Rule:MF_01702};
GN Name=pstB {ECO:0000255|HAMAP-Rule:MF_01702}; Synonyms=mtp1, pstB1;
GN OrderedLocusNames=MSMEG_5779, MSMEI_5626;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=9541026; DOI=10.1016/s0014-5793(98)00214-2;
RA Banerjee S.K., Misra P., Bhatt K., Mande S.C., Chakraborti P.K.;
RT "Identification of an ABC transporter gene that exhibits mRNA level
RT overexpression in fluoroquinolone-resistant Mycobacterium smegmatis.";
RL FEBS Lett. 425:151-156(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
CC -!- FUNCTION: Part of the ABC transporter complex PstSACB involved in
CC phosphate import. Responsible for energy coupling to the transport
CC system. {ECO:0000255|HAMAP-Rule:MF_01702}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + phosphate(out) = ADP + H(+) + 2 phosphate(in);
CC Xref=Rhea:RHEA:24440, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=7.3.2.1;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01702};
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (PstB),
CC two transmembrane proteins (PstC and PstA) and a solute-binding protein
CC (PstS). {ECO:0000255|HAMAP-Rule:MF_01702}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000255|HAMAP-Rule:MF_01702};
CC Peripheral membrane protein {ECO:0000255|HAMAP-Rule:MF_01702}.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Phosphate
CC importer (TC 3.A.1.7) family. {ECO:0000255|HAMAP-Rule:MF_01702}.
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DR EMBL; AF045938; AAC15686.1; -; Genomic_DNA.
DR EMBL; CP000480; ABK72426.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP42061.1; -; Genomic_DNA.
DR RefSeq; WP_011730783.1; NZ_SIJM01000007.1.
DR RefSeq; YP_890008.1; NC_008596.1.
DR AlphaFoldDB; A0R4C0; -.
DR SMR; A0R4C0; -.
DR STRING; 246196.MSMEI_5626; -.
DR PRIDE; A0R4C0; -.
DR EnsemblBacteria; ABK72426; ABK72426; MSMEG_5779.
DR EnsemblBacteria; AFP42061; AFP42061; MSMEI_5626.
DR GeneID; 66737070; -.
DR KEGG; msg:MSMEI_5626; -.
DR KEGG; msm:MSMEG_5779; -.
DR PATRIC; fig|246196.19.peg.5625; -.
DR eggNOG; COG1117; Bacteria.
DR OMA; TIDICRV; -.
DR OrthoDB; 1416748at2; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0015415; F:ATPase-coupled phosphate ion transmembrane transporter activity; IEA:UniProtKB-EC.
DR GO; GO:0005315; F:inorganic phosphate transmembrane transporter activity; IEA:InterPro.
DR CDD; cd03260; ABC_PstB_phosphate_transporter; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005670; Phosp_transpt1.
DR PANTHER; PTHR43423; PTHR43423; 1.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00972; 3a0107s01c2; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR PROSITE; PS51238; PSTB; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell membrane; Membrane; Nucleotide-binding;
KW Phosphate transport; Reference proteome; Translocase; Transport.
FT CHAIN 1..258
FT /note="Phosphate import ATP-binding protein PstB"
FT /id="PRO_0000293600"
FT DOMAIN 5..247
FT /note="ABC transporter"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01702"
FT BINDING 37..44
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01702"
FT CONFLICT 9
FT /note="D -> E (in Ref. 1; AAC15686)"
FT /evidence="ECO:0000305"
FT CONFLICT 16..38
FT /note="AFHAVADVSLAVQPRSVTAFIGP -> GSTRLPTCHSRCSRAASRRIISQ
FT (in Ref. 1; AAC15686)"
FT /evidence="ECO:0000305"
FT CONFLICT 106
FT /note="V -> A (in Ref. 1; AAC15686)"
FT /evidence="ECO:0000305"
FT CONFLICT 174
FT /note="E -> D (in Ref. 1; AAC15686)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 258 AA; 28101 MW; 2613D1CB231C3B9E CRC64;
MAKRLDLKDV NIYYGAFHAV ADVSLAVQPR SVTAFIGPSG CGKSTVLRTL NRMHEVIPGA
RVEGSVLLDG EDIYGPGVDP VGVRKTIGMV FQRPNPFPTM SIRDNVVAGL KLQGVRNKKT
LDEVAERSLR GANLWNEVKD RLDKPGGGLS GGQQQRLCIA RAIAVQPDVL LMDEPCSALD
PISTLAIEDL IATLKLDYTI VIVTHNMQQA ARVSDQTAFF NLEATGKPGR LIEIDDTEKI
FSNPRQKATE DYISGRFG
