PSTB_PSEAE
ID PSTB_PSEAE Reviewed; 277 AA.
AC Q51546;
DT 09-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Phosphate import ATP-binding protein PstB {ECO:0000255|HAMAP-Rule:MF_01702};
DE EC=7.3.2.1 {ECO:0000255|HAMAP-Rule:MF_01702};
DE AltName: Full=ABC phosphate transporter {ECO:0000255|HAMAP-Rule:MF_01702};
DE AltName: Full=Phosphate-transporting ATPase {ECO:0000255|HAMAP-Rule:MF_01702};
GN Name=pstB {ECO:0000255|HAMAP-Rule:MF_01702}; OrderedLocusNames=PA5366;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=8628229; DOI=10.1007/bf02172980;
RA Nikata T., Sakai Y., Shibata K., Kato J., Kuroda A., Ohtake H.;
RT "Molecular analysis of the phosphate-specific transport (pst) operon of
RT Pseudomonas aeruginosa.";
RL Mol. Gen. Genet. 250:692-698(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=8083184; DOI=10.1128/jb.176.18.5874-5877.1994;
RA Kato J., Sakai Y., Nikata T., Ohtake H.;
RT "Cloning and characterization of a Pseudomonas aeruginosa gene involved in
RT the negative regulation of phosphate taxis.";
RL J. Bacteriol. 176:5874-5877(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- FUNCTION: Part of the ABC transporter complex PstSACB involved in
CC phosphate import. Responsible for energy coupling to the transport
CC system. {ECO:0000255|HAMAP-Rule:MF_01702}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O + phosphate(out) = ADP + H(+) + 2 phosphate(in);
CC Xref=Rhea:RHEA:24440, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=7.3.2.1;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01702};
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (PstB),
CC two transmembrane proteins (PstC and PstA) (Potential). PstS is missing
CC in this species. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Peripheral membrane protein.
CC -!- SIMILARITY: Belongs to the ABC transporter superfamily. Phosphate
CC importer (TC 3.A.1.7) family. {ECO:0000255|HAMAP-Rule:MF_01702}.
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DR EMBL; D45195; BAA08137.1; -; Genomic_DNA.
DR EMBL; D28587; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AE004091; AAG08751.1; -; Genomic_DNA.
DR PIR; S68595; S68595.
DR RefSeq; NP_254053.1; NC_002516.2.
DR RefSeq; WP_003096663.1; NZ_QZGE01000020.1.
DR AlphaFoldDB; Q51546; -.
DR SMR; Q51546; -.
DR STRING; 287.DR97_2737; -.
DR PaxDb; Q51546; -.
DR PRIDE; Q51546; -.
DR EnsemblBacteria; AAG08751; AAG08751; PA5366.
DR GeneID; 881628; -.
DR KEGG; pae:PA5366; -.
DR PATRIC; fig|208964.12.peg.5623; -.
DR PseudoCAP; PA5366; -.
DR HOGENOM; CLU_000604_1_22_6; -.
DR InParanoid; Q51546; -.
DR OMA; AFMYMGD; -.
DR PhylomeDB; Q51546; -.
DR BioCyc; PAER208964:G1FZ6-5488-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0015415; F:ATPase-coupled phosphate ion transmembrane transporter activity; IEA:UniProtKB-EC.
DR GO; GO:0005315; F:inorganic phosphate transmembrane transporter activity; IEA:InterPro.
DR CDD; cd03260; ABC_PstB_phosphate_transporter; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR003439; ABC_transporter-like_ATP-bd.
DR InterPro; IPR017871; ABC_transporter-like_CS.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR005670; Phosp_transpt1.
DR PANTHER; PTHR43423; PTHR43423; 1.
DR Pfam; PF00005; ABC_tran; 1.
DR SMART; SM00382; AAA; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR TIGRFAMs; TIGR00972; 3a0107s01c2; 1.
DR PROSITE; PS00211; ABC_TRANSPORTER_1; 1.
DR PROSITE; PS50893; ABC_TRANSPORTER_2; 1.
DR PROSITE; PS51238; PSTB; 1.
PE 3: Inferred from homology;
KW ATP-binding; Cell inner membrane; Cell membrane; Membrane;
KW Nucleotide-binding; Phosphate transport; Reference proteome; Translocase;
KW Transport.
FT CHAIN 1..277
FT /note="Phosphate import ATP-binding protein PstB"
FT /id="PRO_0000092861"
FT DOMAIN 31..272
FT /note="ABC transporter"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01702"
FT BINDING 63..70
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_01702"
SQ SEQUENCE 277 AA; 31045 MW; CC36E7712DD3BFAE CRC64;
MQNETASHGI NFDALGRDRQ SLDLASESVE LEVPGLNLFY GAKQALFDVR MNIPKQRVTA
FIGPSGCGKS TLLRCFNRMN DLVDGCRVEG EIRLDGHNIF AKGVDVAELR RRVGMVFQKP
NPFPKSIYEN VVYGLRIQGI NKKRVLDEAV EWALKGAALW EEVKDRLHES ALGLSGGQQQ
RLVIARTIAV EPEVLLLDEP CSALDPISTL KIEELIYELK SKFTIVIVTH NMQQAARVSD
YTAFMYMGKL IEFGDTDTLF TNPAKKQTED YITGRYG
