PSTK_HUMAN
ID PSTK_HUMAN Reviewed; 348 AA.
AC Q8IV42; Q6ZSS9;
DT 16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT 17-OCT-2006, sequence version 2.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=L-seryl-tRNA(Sec) kinase;
DE EC=2.7.1.164;
DE AltName: Full=O-phosphoseryl-tRNA(Sec) kinase;
GN Name=PSTK; Synonyms=C10orf89;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Corpus callosum;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT ARG-206.
RC TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22814378; DOI=10.1073/pnas.1210303109;
RA Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A.,
RA Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E.,
RA Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R.;
RT "N-terminal acetylome analyses and functional insights of the N-terminal
RT acetyltransferase NatB.";
RL Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012).
CC -!- FUNCTION: Specifically phosphorylates seryl-tRNA(Sec) to O-
CC phosphoseryl-tRNA(Sec), an activated intermediate for selenocysteine
CC biosynthesis. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-tRNA(Sec) = ADP + O-phospho-L-seryl-tRNA(Sec);
CC Xref=Rhea:RHEA:25037, Rhea:RHEA-COMP:9742, Rhea:RHEA-COMP:9947,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:78533, ChEBI:CHEBI:78551,
CC ChEBI:CHEBI:456216; EC=2.7.1.164;
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250};
CC -!- PATHWAY: Aminoacyl-tRNA biosynthesis; selenocysteinyl-tRNA(Sec)
CC biosynthesis; selenocysteinyl-tRNA(Sec) from L-seryl-tRNA(Sec)
CC (archaeal/eukaryal route): step 1/2.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8IV42-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8IV42-2; Sequence=VSP_014989;
CC -!- SIMILARITY: Belongs to the L-seryl-tRNA(Sec) kinase family.
CC {ECO:0000305}.
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DR EMBL; AK127173; BAC86867.1; -; mRNA.
DR EMBL; BC035344; AAH35344.1; -; mRNA.
DR CCDS; CCDS7633.1; -. [Q8IV42-1]
DR RefSeq; NP_699167.2; NM_153336.2. [Q8IV42-1]
DR AlphaFoldDB; Q8IV42; -.
DR STRING; 9606.ENSP00000357882; -.
DR iPTMnet; Q8IV42; -.
DR PhosphoSitePlus; Q8IV42; -.
DR BioMuta; PSTK; -.
DR DMDM; 116242735; -.
DR MassIVE; Q8IV42; -.
DR PaxDb; Q8IV42; -.
DR PeptideAtlas; Q8IV42; -.
DR PRIDE; Q8IV42; -.
DR ProteomicsDB; 70656; -. [Q8IV42-1]
DR ProteomicsDB; 70657; -. [Q8IV42-2]
DR Antibodypedia; 46364; 66 antibodies from 15 providers.
DR DNASU; 118672; -.
DR Ensembl; ENST00000368887.7; ENSP00000357882.3; ENSG00000179988.15. [Q8IV42-1]
DR Ensembl; ENST00000405485.2; ENSP00000384764.2; ENSG00000179988.15. [Q8IV42-1]
DR GeneID; 118672; -.
DR KEGG; hsa:118672; -.
DR UCSC; uc001lgy.2; human. [Q8IV42-1]
DR CTD; 118672; -.
DR DisGeNET; 118672; -.
DR GeneCards; PSTK; -.
DR HGNC; HGNC:28578; PSTK.
DR HPA; ENSG00000179988; Low tissue specificity.
DR MIM; 611310; gene.
DR neXtProt; NX_Q8IV42; -.
DR OpenTargets; ENSG00000179988; -.
DR PharmGKB; PA162400268; -.
DR VEuPathDB; HostDB:ENSG00000179988; -.
DR eggNOG; KOG4622; Eukaryota.
DR GeneTree; ENSGT00390000017554; -.
DR InParanoid; Q8IV42; -.
DR PhylomeDB; Q8IV42; -.
DR TreeFam; TF321264; -.
DR PathwayCommons; Q8IV42; -.
DR Reactome; R-HSA-2408557; Selenocysteine synthesis.
DR UniPathway; UPA00906; UER00897.
DR BioGRID-ORCS; 118672; 379 hits in 1080 CRISPR screens.
DR ChiTaRS; PSTK; human.
DR GenomeRNAi; 118672; -.
DR Pharos; Q8IV42; Tbio.
DR PRO; PR:Q8IV42; -.
DR Proteomes; UP000005640; Chromosome 10.
DR RNAct; Q8IV42; protein.
DR Bgee; ENSG00000179988; Expressed in C1 segment of cervical spinal cord and 136 other tissues.
DR ExpressionAtlas; Q8IV42; baseline and differential.
DR Genevisible; Q8IV42; HS.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016301; F:kinase activity; IBA:GO_Central.
DR GO; GO:0043915; F:L-seryl-tRNA(Sec) kinase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IBA:GO_Central.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:0097056; P:selenocysteinyl-tRNA(Sec) biosynthetic process; IEA:UniProtKB-UniPathway.
DR GO; GO:0006412; P:translation; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR013641; KTI12/PSTK.
DR InterPro; IPR020028; L-seryl-tRNA_Sec_kinase_euk.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF08433; KTI12; 1.
DR SUPFAM; SSF52540; SSF52540; 2.
DR TIGRFAMs; TIGR03575; selen_PSTK_euk; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; ATP-binding; Kinase; Magnesium; Nucleotide-binding;
KW Protein biosynthesis; Reference proteome; Transferase.
FT CHAIN 1..348
FT /note="L-seryl-tRNA(Sec) kinase"
FT /id="PRO_0000097080"
FT BINDING 25..32
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT VAR_SEQ 262..348
FT /note="DTDRIICSTNILHKTDQTLRRIVSQTMKEAKGNQEAFSEMTFKQRWVRANHA
FT AIWRIILGNEHIKCRSAKVGWLQCCRIEKRPLSTG -> VNFQCKFNVNEKEQLFVGRS
FT FEPIIKCE (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_014989"
FT VARIANT 206
FT /note="G -> R (in dbSNP:rs3736582)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_028156"
SQ SEQUENCE 348 AA; 39527 MW; A7DDFF0BDC93BE4E CRC64;
MKTAENIRGT GSDGPRKRGL CVLCGLPAAG KSTFARALAH RLQQEQGWAI GVVAYDDVMP
DAFLAGARAR PAPSQWKLLR QELLKYLEYF LMAVINGCQM SVPPNRTEAM WEDFITCLKD
QDLIFSAAFE AQSCYLLTKT AVSRPLFLVL DDNFYYQSMR YEVYQLARKY SLGFCQLFLD
CPLETCLQRN GQRPQALPPE TIHLMGRKLE KPNPEKNAWE HNSLTIPSPA CASEASLEVT
DLLLTALENP VKYAEDNMEQ KDTDRIICST NILHKTDQTL RRIVSQTMKE AKGNQEAFSE
MTFKQRWVRA NHAAIWRIIL GNEHIKCRSA KVGWLQCCRI EKRPLSTG
