ID   PSTS2_MYCTO             Reviewed;         370 AA.
AC   P9WGT8; L0T576; O05870; P96905;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 35.
DE   RecName: Full=Phosphate-binding protein PstS 2;
DE            Short=PBP 2;
DE            Short=PstS-2;
DE   Flags: Precursor;
GN   Name=pstS2; OrderedLocusNames=MT0959;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Part of the ABC transporter complex PstSACB involved in
CC       phosphate import. {ECO:0000250}.
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (PstB),
CC       two transmembrane proteins (PstC and PstA) and a solute-binding protein
CC       (PstS). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the PstS family. {ECO:0000305}.
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DR   EMBL; AE000516; AAK45206.1; -; Genomic_DNA.
DR   PIR; D70584; D70584.
DR   RefSeq; WP_003898652.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WGT8; -.
DR   SMR; P9WGT8; -.
DR   EnsemblBacteria; AAK45206; AAK45206; MT0959.
DR   KEGG; mtc:MT0959; -.
DR   PATRIC; fig|83331.31.peg.1029; -.
DR   HOGENOM; CLU_034528_0_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR   GO; GO:0042301; F:phosphate ion binding; IEA:InterPro.
DR   GO; GO:0035435; P:phosphate ion transmembrane transport; IEA:InterPro.
DR   InterPro; IPR005673; ABC_phos-bd_PstS.
DR   InterPro; IPR024370; PBP_domain.
DR   Pfam; PF12849; PBP_like_2; 1.
DR   PIRSF; PIRSF002756; PstS; 1.
DR   TIGRFAMs; TIGR00975; 3a0107s03; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Lipoprotein; Membrane; Palmitate; Phosphate transport;
KW   Signal; Transport.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           23..370
FT                   /note="Phosphate-binding protein PstS 2"
FT                   /id="PRO_0000428304"
FT   BINDING         54..56
FT                   /ligand="phosphate"
FT                   /ligand_id="ChEBI:CHEBI:43474"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT7"
FT   BINDING         84
FT                   /ligand="phosphate"
FT                   /ligand_id="ChEBI:CHEBI:43474"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT7"
FT   BINDING         102
FT                   /ligand="phosphate"
FT                   /ligand_id="ChEBI:CHEBI:43474"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT7"
FT   BINDING         191..193
FT                   /ligand="phosphate"
FT                   /ligand_id="ChEBI:CHEBI:43474"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT7"
FT   LIPID           23
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           23
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   370 AA;  37864 MW;  97F5116CEE9B3B5C CRC64;
     MKFARSGAAV SLLAAGTLVL TACGGGTNSS SSGAGGTSGS VHCGGKKELH SSGSTAQENA
     MEQFVYAYVR SCPGYTLDYN ANGSGAGVTQ FLNNETDFAG SDVPLNPSTG QPDRSAERCG
     SPAWDLPTVF GPIAITYNIK GVSTLNLDGP TTAKIFNGTI TVWNDPQIQA LNSGTDLPPT
     PISVIFRSDK SGTSDNFQKY LDGASNGAWG KGASETFNGG VGVGASGNNG TSALLQTTDG
     SITYNEWSFA VGKQLNMAQI ITSAGPDPVA ITTESVGKTI AGAKIMGQGN DLVLDTSSFY
     RPTQPGSYPI VLATYEIVCS KYPDATTGTA VRAFMQAAIG PGQEGLDQYG SIPLPKSFQA
     KLAAAVNAIS