ID   PSTS3_MYCTO             Reviewed;         370 AA.
AC   P9WGT6; L0T5B5; O86343; P0A5Y2; Q50794;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 1.
DT   03-AUG-2022, entry version 32.
DE   RecName: Full=Phosphate-binding protein PstS 3;
DE            Short=PBP 3;
DE            Short=PstS-3;
DE   AltName: Full=Antigen Ag88;
DE   Flags: Precursor;
GN   Name=pstS3; Synonyms=phoS2; OrderedLocusNames=MT0955;
OS   Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycobacterium; Mycobacterium tuberculosis complex.
OX   NCBI_TaxID=83331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CDC 1551 / Oshkosh;
RX   PubMed=12218036; DOI=10.1128/jb.184.19.5479-5490.2002;
RA   Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O.,
RA   Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K.,
RA   Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L.,
RA   Delcher A., Utterback T.R., Weidman J.F., Khouri H.M., Gill J., Mikula A.,
RA   Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M.;
RT   "Whole-genome comparison of Mycobacterium tuberculosis clinical and
RT   laboratory strains.";
RL   J. Bacteriol. 184:5479-5490(2002).
CC   -!- FUNCTION: Part of the ABC transporter complex PstSACB involved in
CC       phosphate import. {ECO:0000250|UniProtKB:P9WGT7}.
CC   -!- SUBUNIT: The complex is composed of two ATP-binding proteins (PstB),
CC       two transmembrane proteins (PstC and PstA) and a solute-binding protein
CC       (PstS). {ECO:0000250|UniProtKB:P9WGT7}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the PstS family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAK45202.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; AE000516; AAK45202.1; ALT_INIT; Genomic_DNA.
DR   PIR; H70583; H70583.
DR   RefSeq; WP_003404775.1; NZ_KK341227.1.
DR   AlphaFoldDB; P9WGT6; -.
DR   SMR; P9WGT6; -.
DR   EnsemblBacteria; AAK45202; AAK45202; MT0955.
DR   GeneID; 45424894; -.
DR   KEGG; mtc:MT0955; -.
DR   PATRIC; fig|83331.31.peg.1025; -.
DR   HOGENOM; CLU_034528_0_0_11; -.
DR   Proteomes; UP000001020; Chromosome.
DR   GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR   GO; GO:0042301; F:phosphate ion binding; IEA:InterPro.
DR   GO; GO:0035435; P:phosphate ion transmembrane transport; IEA:InterPro.
DR   InterPro; IPR005673; ABC_phos-bd_PstS.
DR   InterPro; IPR024370; PBP_domain.
DR   Pfam; PF12849; PBP_like_2; 1.
DR   PIRSF; PIRSF002756; PstS; 1.
DR   TIGRFAMs; TIGR00975; 3a0107s03; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   3: Inferred from homology;
KW   Cell membrane; Lipoprotein; Membrane; Palmitate; Phosphate transport;
KW   Signal; Stress response; Transport.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           23..370
FT                   /note="Phosphate-binding protein PstS 3"
FT                   /id="PRO_0000428305"
FT   BINDING         56..58
FT                   /ligand="phosphate"
FT                   /ligand_id="ChEBI:CHEBI:43474"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT7"
FT   BINDING         86
FT                   /ligand="phosphate"
FT                   /ligand_id="ChEBI:CHEBI:43474"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT7"
FT   BINDING         104
FT                   /ligand="phosphate"
FT                   /ligand_id="ChEBI:CHEBI:43474"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT7"
FT   BINDING         191..193
FT                   /ligand="phosphate"
FT                   /ligand_id="ChEBI:CHEBI:43474"
FT                   /evidence="ECO:0000250|UniProtKB:P9WGT7"
FT   LIPID           23
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           23
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   370 AA;  37953 MW;  7D557829A9A118E0 CRC64;
     MKLNRFGAAV GVLAAGALVL SACGNDDNVT GGGATTGQAS AKVDCGGKKT LKASGSTAQA
     NAMTRFVNVF EQACPGQTLN YTANGSGAGI SEFNGNQTDF GGSDVPLSKD EAAAAQRRCG
     SPAWNLPVVF GPIAVTYNLN SVSSLNLDGP TLAKIFNGSI TQWNNPAIQA LNRDFTLPGE
     RIHVVFRSDE SGTTDNFQRY LQAASNGAWG KGAGKSFQGG VGEGARGNDG TSAAAKNTPG
     SITYNEWSFA QAQHLTMANI VTSAGGDPVA ITIDSVGQTI AGATISGVGN DLVLDTDSFY
     RPKRPGSYPI VLATYEIVCS KYPDSQVGTA VKAFLQSTIG AGQSGLGDNG YIPIPDEFKS
     RLSTAVNAIA