PSTS_BACSU
ID PSTS_BACSU Reviewed; 300 AA.
AC P46338;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=Phosphate-binding protein PstS;
DE Short=PBP;
DE Flags: Precursor;
GN Name=pstS; Synonyms=yqgG, yzmB; OrderedLocusNames=BSU24990;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / JH642;
RX PubMed=8760913; DOI=10.1099/13500872-142-8-2017;
RA Takemaru K., Mizuno M., Kobayashi Y.;
RT "A Bacillus subtilis gene cluster similar to the Escherichia coli
RT phosphate-specific transport (pst) operon: evidence for a tandemly arranged
RT pstB gene.";
RL Microbiology 142:2017-2020(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / JH642;
RX PubMed=8969508; DOI=10.1099/13500872-142-11-3103;
RA Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA Kobayashi Y.;
RT "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the
RT Bacillus subtilis genome containing the skin element and many sporulation
RT genes.";
RL Microbiology 142:3103-3111(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
CC -!- FUNCTION: Part of the ABC transporter complex PstSACB involved in
CC phosphate import. {ECO:0000250}.
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (PstB),
CC two transmembrane proteins (PstC and PstA) and a solute-binding protein
CC (PstS). {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the PstS family. {ECO:0000305}.
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DR EMBL; D58414; BAA09581.1; -; Genomic_DNA.
DR EMBL; D84432; BAA12510.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB14429.1; -; Genomic_DNA.
DR PIR; A69956; A69956.
DR RefSeq; NP_390378.1; NC_000964.3.
DR RefSeq; WP_004398774.1; NZ_JNCM01000036.1.
DR AlphaFoldDB; P46338; -.
DR SMR; P46338; -.
DR STRING; 224308.BSU24990; -.
DR PaxDb; P46338; -.
DR PRIDE; P46338; -.
DR EnsemblBacteria; CAB14429; CAB14429; BSU_24990.
DR GeneID; 938197; -.
DR KEGG; bsu:BSU24990; -.
DR PATRIC; fig|224308.179.peg.2718; -.
DR eggNOG; COG0226; Bacteria.
DR InParanoid; P46338; -.
DR OMA; KMGYIPM; -.
DR PhylomeDB; P46338; -.
DR BioCyc; BSUB:BSU24990-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0042301; F:phosphate ion binding; IEA:InterPro.
DR GO; GO:0006817; P:phosphate ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR024370; PBP_domain.
DR InterPro; IPR011862; Phos-bd.
DR Pfam; PF12849; PBP_like_2; 1.
DR TIGRFAMs; TIGR02136; ptsS_2; 1.
DR PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE 3: Inferred from homology;
KW Cell membrane; Lipoprotein; Membrane; Palmitate; Phosphate transport;
KW Reference proteome; Signal; Transport.
FT SIGNAL 1..21
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT CHAIN 22..300
FT /note="Phosphate-binding protein PstS"
FT /id="PRO_0000031862"
FT REGION 27..47
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 22
FT /note="N-palmitoyl cysteine"
FT /evidence="ECO:0000305"
FT LIPID 22
FT /note="S-diacylglycerol cysteine"
FT /evidence="ECO:0000305"
SQ SEQUENCE 300 AA; 31684 MW; 9DBA6090947A277B CRC64;
MKKNKLVLML LMAAFMMIAA ACGNAGESKK SNSDSAKGEE KASGSLTISG SSAMQPLVLA
AAEKFMEENP DADIQVQAGG SGTGLSQVSE GAVQIGNSDV FAEEKEGIDA KALVDHQVAV
VGMAAAVNPD AGVKDISKDE LKKIFTGKIK NWKELGGKDQ KITLVNRPDS SGTRATFVKY
ALDGAEPAEG ITEDSSNTVK KIIADTPGAI GYLAFSYLTD DKVTALSIDG VKPEAKNVAT
GEYPIWAYQH SYTKGEATGL AKEFLDYLKS EDIQKSIVTD QGYIPVTDMK VTRDANGKQS
