PSTS_SHIFL
ID PSTS_SHIFL Reviewed; 346 AA.
AC P0AG83; P06128; P76744;
DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1988, sequence version 1.
DT 03-AUG-2022, entry version 99.
DE RecName: Full=Phosphate-binding protein PstS;
DE Short=PBP;
DE Flags: Precursor;
GN Name=pstS; OrderedLocusNames=SF3727, S4045;
OS Shigella flexneri.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Shigella.
OX NCBI_TaxID=623;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=301 / Serotype 2a;
RX PubMed=12384590; DOI=10.1093/nar/gkf566;
RA Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J.,
RA Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L.,
RA Xue Y., Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H.,
RA Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.;
RT "Genome sequence of Shigella flexneri 2a: insights into pathogenicity
RT through comparison with genomes of Escherichia coli K12 and O157.";
RL Nucleic Acids Res. 30:4432-4441(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700930 / 2457T / Serotype 2a;
RX PubMed=12704152; DOI=10.1128/iai.71.5.2775-2786.2003;
RA Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G.,
RA Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T.,
RA Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.;
RT "Complete genome sequence and comparative genomics of Shigella flexneri
RT serotype 2a strain 2457T.";
RL Infect. Immun. 71:2775-2786(2003).
CC -!- FUNCTION: Part of the ABC transporter complex PstSACB involved in
CC phosphate import. {ECO:0000250}.
CC -!- SUBUNIT: The complex is composed of two ATP-binding proteins (PstB),
CC two transmembrane proteins (PstC and PstA) and a solute-binding protein
CC (PstS). {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the PstS family. {ECO:0000305}.
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DR EMBL; AE005674; AAN45173.1; -; Genomic_DNA.
DR EMBL; AE014073; AAP19025.1; -; Genomic_DNA.
DR RefSeq; NP_709466.1; NC_004337.2.
DR RefSeq; WP_000867146.1; NZ_WPGW01000131.1.
DR AlphaFoldDB; P0AG83; -.
DR SMR; P0AG83; -.
DR STRING; 198214.SF3727; -.
DR EnsemblBacteria; AAN45173; AAN45173; SF3727.
DR EnsemblBacteria; AAP19025; AAP19025; S4045.
DR GeneID; 1026179; -.
DR GeneID; 66672372; -.
DR KEGG; sfl:SF3727; -.
DR KEGG; sfx:S4045; -.
DR PATRIC; fig|198214.7.peg.4400; -.
DR HOGENOM; CLU_034528_1_0_6; -.
DR OMA; FPYPVYA; -.
DR OrthoDB; 1222928at2; -.
DR Proteomes; UP000001006; Chromosome.
DR Proteomes; UP000002673; Chromosome.
DR GO; GO:0043190; C:ATP-binding cassette (ABC) transporter complex; IEA:InterPro.
DR GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR GO; GO:0042301; F:phosphate ion binding; IEA:InterPro.
DR GO; GO:0035435; P:phosphate ion transmembrane transport; IEA:InterPro.
DR InterPro; IPR005673; ABC_phos-bd_PstS.
DR InterPro; IPR024370; PBP_domain.
DR Pfam; PF12849; PBP_like_2; 1.
DR PIRSF; PIRSF002756; PstS; 1.
DR TIGRFAMs; TIGR00975; 3a0107s03; 1.
PE 3: Inferred from homology;
KW Periplasm; Phosphate transport; Reference proteome; Signal; Transport.
FT SIGNAL 1..25
FT /evidence="ECO:0000250"
FT CHAIN 26..346
FT /note="Phosphate-binding protein PstS"
FT /id="PRO_0000045263"
FT BINDING 34..36
FT /ligand="phosphate"
FT /ligand_id="ChEBI:CHEBI:43474"
FT /evidence="ECO:0000250|UniProtKB:P9WGT7"
FT BINDING 63
FT /ligand="phosphate"
FT /ligand_id="ChEBI:CHEBI:43474"
FT /evidence="ECO:0000250|UniProtKB:P9WGT7"
FT BINDING 81
FT /ligand="phosphate"
FT /ligand_id="ChEBI:CHEBI:43474"
FT /evidence="ECO:0000250|UniProtKB:P9WGT7"
FT BINDING 164..166
FT /ligand="phosphate"
FT /ligand_id="ChEBI:CHEBI:43474"
FT /evidence="ECO:0000250|UniProtKB:P9WGT7"
SQ SEQUENCE 346 AA; 37024 MW; 867DA7199C2C87ED CRC64;
MKVMRTTVAT VVAATLSMSA FSVFAEASLT GAGATFPAPV YAKWADTYQK ETGNKVNYQG
IGSSGGVKQI IANTVDFGAS DAPLSDEKLA QEGLFQFPTV IGGVVLAVNI PGLKSGELVL
DGKTLGDIYL GKIKKWDDEA IAKLNPGLKL PSQNIAVVRR ADGSGTSFVF TSYLAKVNEE
WKNNVGTGST VKWPIGLGGK GNDGIAAFVQ RLPGAIGYVE YAYAKQNNLA YTKLISADGK
PVSPTEENFA NAAKGADWSK TFAQDLTNQK GEDAWPITST TFILIHKDQK KPEQGTEVLK
FFDWAYKTGA KQANDLDYAS LPDSVVEQVR AAWKTNIKDS SGKPLY