ID   AACUR_ASPA1             Reviewed;         363 AA.
AC   A0A1L9WLK2;
DT   29-SEP-2021, integrated into UniProtKB/Swiss-Prot.
DT   15-MAR-2017, sequence version 1.
DT   03-AUG-2022, entry version 21.
DE   RecName: Full=Transcriptional regulator AacuR {ECO:0000303|PubMed:30996871};
DE   AltName: Full=Secalonic acid cluster protein R {ECO:0000303|PubMed:30996871};
GN   Name=AacuR {ECO:0000303|PubMed:30996871}; ORFNames=ASPACDRAFT_1890825;
OS   Aspergillus aculeatus (strain ATCC 16872 / CBS 172.66 / WB 5094).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus;
OC   Aspergillus subgen. Circumdati.
OX   NCBI_TaxID=690307;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 16872 / CBS 172.66 / WB 5094;
RX   PubMed=28196534; DOI=10.1186/s13059-017-1151-0;
RA   de Vries R.P., Riley R., Wiebenga A., Aguilar-Osorio G., Amillis S.,
RA   Uchima C.A., Anderluh G., Asadollahi M., Askin M., Barry K., Battaglia E.,
RA   Bayram O., Benocci T., Braus-Stromeyer S.A., Caldana C., Canovas D.,
RA   Cerqueira G.C., Chen F., Chen W., Choi C., Clum A., Dos Santos R.A.,
RA   Damasio A.R., Diallinas G., Emri T., Fekete E., Flipphi M., Freyberg S.,
RA   Gallo A., Gournas C., Habgood R., Hainaut M., Harispe M.L., Henrissat B.,
RA   Hilden K.S., Hope R., Hossain A., Karabika E., Karaffa L., Karanyi Z.,
RA   Krasevec N., Kuo A., Kusch H., LaButti K., Lagendijk E.L., Lapidus A.,
RA   Levasseur A., Lindquist E., Lipzen A., Logrieco A.F., MacCabe A.,
RA   Maekelae M.R., Malavazi I., Melin P., Meyer V., Mielnichuk N., Miskei M.,
RA   Molnar A.P., Mule G., Ngan C.Y., Orejas M., Orosz E., Ouedraogo J.P.,
RA   Overkamp K.M., Park H.-S., Perrone G., Piumi F., Punt P.J., Ram A.F.,
RA   Ramon A., Rauscher S., Record E., Riano-Pachon D.M., Robert V., Roehrig J.,
RA   Ruller R., Salamov A., Salih N.S., Samson R.A., Sandor E., Sanguinetti M.,
RA   Schuetze T., Sepcic K., Shelest E., Sherlock G., Sophianopoulou V.,
RA   Squina F.M., Sun H., Susca A., Todd R.B., Tsang A., Unkles S.E.,
RA   van de Wiele N., van Rossen-Uffink D., Oliveira J.V., Vesth T.C.,
RA   Visser J., Yu J.-H., Zhou M., Andersen M.R., Archer D.B., Baker S.E.,
RA   Benoit I., Brakhage A.A., Braus G.H., Fischer R., Frisvad J.C.,
RA   Goldman G.H., Houbraken J., Oakley B., Pocsi I., Scazzocchio C.,
RA   Seiboth B., vanKuyk P.A., Wortman J., Dyer P.S., Grigoriev I.V.;
RT   "Comparative genomics reveals high biological diversity and specific
RT   adaptations in the industrially and medically important fungal genus
RT   Aspergillus.";
RL   Genome Biol. 18:RESEARCH28.1-RESEARCH28.45(2017).
RN   [2]
RP   BIOTECHNOLOGY.
RX   PubMed=28253713; DOI=10.4149/neo_2017_304;
RA   Gao X., Sun H.L., Liu D.S., Zhang J.R., Zhang J., Yan M.M., Pan X.H.;
RT   "Secalonic acid- F inhibited cell growth more effectively than 5-
RT   fluorouracil on hepatocellular carcinoma in vitro and in vivo.";
RL   Neoplasma 64:344-350(2017).
RN   [3]
RP   BIOTECHNOLOGY.
RX   PubMed=29248948; DOI=10.1007/s00284-017-1411-y;
RA   Yodsing N., Lekphrom R., Sangsopha W., Aimi T., Boonlue S.;
RT   "Secondary Metabolites and Their Biological Activity from Aspergillus
RT   aculeatus KKU-CT2.";
RL   Curr. Microbiol. 75:513-518(2018).
RN   [4]
RP   IDENTIFICATION, AND FUNCTION.
RX   PubMed=30996871; DOI=10.1039/c8sc05126g;
RA   Greco C., de Mattos-Shipley K., Bailey A.M., Mulholland N.P., Vincent J.L.,
RA   Willis C.L., Cox R.J., Simpson T.J.;
RT   "Structure revision of cryptosporioptides and determination of the genetic
RT   basis for dimeric xanthone biosynthesis in fungi.";
RL   Chem. Sci. 10:2930-2939(2019).
RN   [5]
RP   BIOTECHNOLOGY.
RX   PubMed=30678274; DOI=10.3390/molecules24030393;
RA   Xie L., Li M., Liu D., Wang X., Wang P., Dai H., Yang W., Liu W., Hu X.,
RA   Zhao M.;
RT   "Secalonic Acid-F, a Novel Mycotoxin, Represses the Progression of
RT   Hepatocellular Carcinoma via MARCH1 Regulation of the PI3K/AKT/beta-catenin
RT   Signaling Pathway.";
RL   Molecules 24:0-0(2019).
RN   [6]
RP   BIOTECHNOLOGY.
RX   PubMed=33015446; DOI=10.1021/acsomega.0c02505;
RA   Farooq S., Qayum A., Nalli Y., Lauro G., Chini M.G., Bifulco G.,
RA   Chaubey A., Singh S.K., Riyaz-Ul-Hassan S., Ali A.;
RT   "Discovery of a Secalonic Acid Derivative from Aspergillus aculeatus, an
RT   Endophyte of Rosa damascena Mill., Triggers Apoptosis in MDA-MB-231 Triple
RT   Negative Breast Cancer Cells.";
RL   ACS Omega 5:24296-24310(2020).
RN   [7]
RP   FUNCTION.
RX   PubMed=33891392; DOI=10.1021/acs.jnatprod.1c00022;
RA   Wei X., Chen X., Chen L., Yan D., Wang W.G., Matsuda Y.;
RT   "Heterologous biosynthesis of tetrahydroxanthone dimers: determination of
RT   key factors for selective or divergent synthesis.";
RL   J. Nat. Prod. 84:1544-1549(2021).
CC   -!- FUNCTION: Transcriptional regulator; part of the gene cluster that
CC       mediates the biosynthesis of the tetrahydroxanthone dimer secalonic
CC       acid D. {ECO:0000269|PubMed:30996871, ECO:0000269|PubMed:33891392}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
CC   -!- BIOTECHNOLOGY: Secalonic acids show unprecedented anticancer activities
CC       against various human cancer cells and might be interesting for further
CC       derivatization, targeting diseases such as cancer.
CC       {ECO:0000269|PubMed:28253713, ECO:0000269|PubMed:29248948,
CC       ECO:0000269|PubMed:30678274, ECO:0000269|PubMed:33015446}.
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DR   EMBL; KV878984; OJJ97037.1; -; Genomic_DNA.
DR   RefSeq; XP_020053377.1; XM_020198592.1.
DR   SMR; A0A1L9WLK2; -.
DR   EnsemblFungi; OJJ97037; OJJ97037; ASPACDRAFT_1890825.
DR   GeneID; 30972406; -.
DR   VEuPathDB; FungiDB:ASPACDRAFT_1890825; -.
DR   OrthoDB; 1396569at2759; -.
DR   Proteomes; UP000184546; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0045122; P:aflatoxin biosynthetic process; IEA:InterPro.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR013700; AflR.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   Pfam; PF08493; AflR; 1.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS00463; ZN2_CY6_FUNGAL_1; 1.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Metal-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation; Zinc.
FT   CHAIN           1..363
FT                   /note="Transcriptional regulator AacuR"
FT                   /id="PRO_0000453506"
FT   DNA_BIND        27..54
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   REGION          1..24
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          63..104
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        79..104
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   363 AA;  40442 MW;  AA499DBE64883FFD CRC64;
     MTSLQCPPPD RTRRSLSPTG PKFRESCKSC AASKIRCTKE KPQCARCVKR NMVCEYLESK
     RARRKPGARL KHRESITTNA HHSPTTTTIT TSRTTSSSPS ASPKTYHPLN YVDILSWTPT
     PTLPDSATDT QTGLHCNFDD FLAFSHSTSL LGVPEAGELP VHLTTDFRQL EELNRAPEAL
     DEILQCRCSE DGYQLAILSV VILKVLGWYA TIIRPLLGVE AHSPGVGEGT TVRTDSLAET
     QLSSSFYVDS GQQISPKITM GPPLAYGRES EGKTRMAAQL ILSQLHRVQR LVNILCHRFK
     LHSNREQNPA WNSSTASTAT EPEGPAYIEH LYPFSSLVFD QIEKDVRGRL RSLSTELLEM
     LRY