PSUT_ECOLI
ID PSUT_ECOLI Reviewed; 416 AA.
AC P33024; Q2MAR5;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1994, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Putative pseudouridine transporter;
GN Name=psuT; Synonyms=pscT, yeiM; OrderedLocusNames=b2164, JW2151;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / BHB2600;
RA Richterich P., Lakey N., Gryan G., Jaehn L., Mintz L., Robison K.,
RA Church G.M.;
RT "Automated multiplex sequencing of the E.coli genome.";
RL Submitted (OCT-1993) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
CC -!- FUNCTION: Could be involved in pseudouridine transport.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the concentrative nucleoside transporter (CNT)
CC (TC 2.A.41) family. {ECO:0000305}.
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DR EMBL; U00007; AAA60518.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75225.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE76641.1; -; Genomic_DNA.
DR PIR; C64985; C64985.
DR RefSeq; NP_416669.1; NC_000913.3.
DR RefSeq; WP_000353878.1; NZ_SSZK01000027.1.
DR AlphaFoldDB; P33024; -.
DR SMR; P33024; -.
DR BioGRID; 4260636; 134.
DR DIP; DIP-11925N; -.
DR IntAct; P33024; 2.
DR STRING; 511145.b2164; -.
DR TCDB; 2.A.41.2.9; the concentrative nucleoside transporter (cnt) family.
DR PaxDb; P33024; -.
DR PRIDE; P33024; -.
DR EnsemblBacteria; AAC75225; AAC75225; b2164.
DR EnsemblBacteria; BAE76641; BAE76641; BAE76641.
DR GeneID; 946671; -.
DR KEGG; ecj:JW2151; -.
DR KEGG; eco:b2164; -.
DR PATRIC; fig|1411691.4.peg.75; -.
DR EchoBASE; EB1967; -.
DR eggNOG; COG1972; Bacteria.
DR HOGENOM; CLU_016813_4_2_6; -.
DR InParanoid; P33024; -.
DR OMA; IMLYAMC; -.
DR PhylomeDB; P33024; -.
DR BioCyc; EcoCyc:YEIM-MON; -.
DR PRO; PR:P33024; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0005337; F:nucleoside transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015293; F:symporter activity; IBA:GO_Central.
DR GO; GO:1901642; P:nucleoside transmembrane transport; IBA:GO_Central.
DR InterPro; IPR008276; C_nuclsd_transpt.
DR InterPro; IPR018270; C_nuclsd_transpt_met_bac.
DR InterPro; IPR011657; CNT_C_dom.
DR InterPro; IPR002668; CNT_N_dom.
DR PANTHER; PTHR10590; PTHR10590; 1.
DR Pfam; PF07662; Nucleos_tra2_C; 1.
DR Pfam; PF01773; Nucleos_tra2_N; 1.
DR TIGRFAMs; TIGR00804; nupC; 1.
PE 1: Evidence at protein level;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..416
FT /note="Putative pseudouridine transporter"
FT /id="PRO_0000070458"
FT TOPO_DOM 1..2
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 3..23
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 24..31
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 32..52
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 53..104
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 105..125
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 126..172
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 173..193
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 194..196
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 197..217
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 218..268
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 269..289
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 290..352
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 353..373
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 374..394
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 395..415
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 416
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 416 AA; 43432 MW; 60CCE29DBF46722A CRC64;
MDIMRSVVGM VVLLAIAFLL SVNKKSISLR TVGAALLLQI AIGGIMLYFP PGKWAVEQAA
LGVHKVMSYS DAGSAFIFGS LVGPKMDVLF DGAGFIFAFR VLPAIIFVTA LISLLYYIGV
MGLLIRILGS IFQKALNISK IESFVAVTTI FLGQNEIPAI VKPFIDRMNR NELFTAICSG
MASIAGSMMI GYAGMGVPID YLLAASLMAI PGGILFARIL SPATEPSQVT FENLSFSETP
PKSFIEAAAS GAMTGLKIAA GVATVVMAFV AIIALINGII GGIGGWFGFA NASLESIFGY
VLAPLAWIMG VDWSDANLAG SLIGQKLAIN EFVAYLSFSP YLQTGGTLEV KTIAIISFAL
CGFANFGSIG VVVGAFSAIS PKRAPEIAQL GLRALAAATL SNLMSATIAG FFIGLA
