PSY1_ARATH
ID PSY1_ARATH Reviewed; 75 AA.
AC Q941C7;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Protein PSY1 {ECO:0000303|PubMed:17989228};
DE Contains:
DE RecName: Full=Tyrosine-sulfated glycopeptide 1 {ECO:0000303|PubMed:17989228};
DE Flags: Precursor;
GN Name=PSY1 {ECO:0000303|PubMed:17989228};
GN OrderedLocusNames=At5g58650 {ECO:0000312|Araport:AT5G58650};
GN ORFNames=MZN1.100 {ECO:0000312|EMBL:AB020755};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 48-65, SULFATION AT TYR-49,
RP HYDROXYLATION AT PRO-63 AND PRO-64, GLYCOSYLATION AT PRO-63, FUNCTION,
RP TISSUE SPECIFICITY, INDUCTION, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=cv. Columbia;
RX PubMed=17989228; DOI=10.1073/pnas.0706403104;
RA Amano Y., Tsubouchi H., Shinohara H., Ogawa M., Matsubayashi Y.;
RT "Tyrosine-sulfated glycopeptide involved in cellular proliferation and
RT expansion in Arabidopsis.";
RL Proc. Natl. Acad. Sci. U.S.A. 104:18333-18338(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION.
RX PubMed=25267325; DOI=10.1111/tpj.12680;
RA Fuglsang A.T., Kristensen A., Cuin T.A., Schulze W.X., Persson J.,
RA Thuesen K.H., Ytting C.K., Oehlenschlaeger C.B., Mahmood K.,
RA Sondergaard T.E., Shabala S., Palmgren M.G.;
RT "Receptor kinase-mediated control of primary active proton pumping at the
RT plasma membrane.";
RL Plant J. 80:951-964(2014).
CC -!- FUNCTION: Promotes cellular proliferation and expansion
CC (PubMed:17989228). Induces outward H(+) fluxes in roots
CC (PubMed:25267325). {ECO:0000269|PubMed:17989228,
CC ECO:0000269|PubMed:25267325}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305|PubMed:17989228}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, stems, leaves, flowers and
CC shoot apical meristems. {ECO:0000269|PubMed:17989228}.
CC -!- INDUCTION: By wounding. {ECO:0000269|PubMed:17989228}.
CC -!- PTM: Glycosylated with 3 L-arabinose units.
CC {ECO:0000269|PubMed:17989228}.
CC -!- PTM: The sulfation and the L-Ara(3) chain are required for full
CC activity. {ECO:0000269|PubMed:17989228}.
CC -!- SIMILARITY: Belongs to the sulfated-peptide plant hormone family.
CC {ECO:0000305}.
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DR EMBL; AB304257; BAF91447.1; -; mRNA.
DR EMBL; AB020755; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002688; AED97081.1; -; Genomic_DNA.
DR EMBL; AY052256; AAK97726.1; -; mRNA.
DR EMBL; AY132002; AAM91035.1; -; mRNA.
DR RefSeq; NP_200673.1; NM_125252.1.
DR AlphaFoldDB; Q941C7; -.
DR BioGRID; 21223; 3.
DR IntAct; Q941C7; 3.
DR STRING; 3702.AT5G58650.1; -.
DR PaxDb; Q941C7; -.
DR PRIDE; Q941C7; -.
DR EnsemblPlants; AT5G58650.1; AT5G58650.1; AT5G58650.
DR GeneID; 835979; -.
DR Gramene; AT5G58650.1; AT5G58650.1; AT5G58650.
DR KEGG; ath:AT5G58650; -.
DR Araport; AT5G58650; -.
DR TAIR; locus:2178773; AT5G58650.
DR HOGENOM; CLU_197080_1_0_1; -.
DR OMA; NPKHDPG; -.
DR OrthoDB; 1629875at2759; -.
DR PRO; PR:Q941C7; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q941C7; baseline and differential.
DR Genevisible; Q941C7; AT.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0008083; F:growth factor activity; IEA:UniProtKB-KW.
DR GO; GO:0009658; P:chloroplast organization; IMP:TAIR.
DR GO; GO:0042127; P:regulation of cell population proliferation; IMP:TAIR.
DR InterPro; IPR034430; PSY.
DR PANTHER; PTHR37177; PTHR37177; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Glycoprotein; Growth factor; Hydroxylation;
KW Reference proteome; Secreted; Signal; Sulfation.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT PROPEP 23..47
FT /evidence="ECO:0000255"
FT /id="PRO_0000365609"
FT PEPTIDE 48..65
FT /note="Tyrosine-sulfated glycopeptide 1"
FT /id="PRO_0000365610"
FT PROPEP 66..75
FT /evidence="ECO:0000255"
FT /id="PRO_0000365611"
FT REGION 48..75
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 49
FT /note="Sulfotyrosine"
FT /evidence="ECO:0000269|PubMed:17989228"
FT MOD_RES 63
FT /note="4-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:17989228"
FT MOD_RES 64
FT /note="4-hydroxyproline"
FT /evidence="ECO:0000269|PubMed:17989228"
FT CARBOHYD 63
FT /note="O-linked (Ara...) hydroxyproline"
FT /evidence="ECO:0000269|PubMed:17989228"
SQ SEQUENCE 75 AA; 7928 MW; BC2161A53E208EA6 CRC64;
MTFVVRLLVC LLLTLTITSS LARNPVSVSG GFENSGFQRS LLMVNVEDYG DPSANPKHDP
GVPPSATGQR VVGRG
