PSY1_SOLLC
ID PSY1_SOLLC Reviewed; 412 AA.
AC P08196; Q40166;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 2.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Phytoene synthase 1, chloroplastic;
DE EC=2.5.1.32;
DE AltName: Full=Fruit-ripening-specific protein pTOM5;
DE Flags: Precursor;
GN Name=PSY1; Synonyms=GTOM5, PTOM5, TOM5;
OS Solanum lycopersicum (Tomato) (Lycopersicon esculentum).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum;
OC Solanum subgen. Lycopersicon.
OX NCBI_TaxID=4081;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND CHARACTERIZATION.
RX PubMed=1544888; DOI=10.1016/s0021-9258(18)42724-x;
RA Bartley G.E., Viitanen P.V., Bacot K.O., Scolnik P.A.;
RT "A tomato gene expressed during fruit ripening encodes an enzyme of the
RT carotenoid biosynthesis pathway.";
RL J. Biol. Chem. 267:5036-5039(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. Marmande;
RX PubMed=8250898; DOI=10.1006/bbrc.1993.2410;
RA Roemer S., Hugueney P., Bouvier F., Camara B., Kuntz M.;
RT "Expression of the genes encoding the early carotenoid biosynthetic enzymes
RT in Capsicum annuum.";
RL Biochem. Biophys. Res. Commun. 196:1414-1421(1993).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Ailsa Craig;
RX PubMed=3697097; DOI=10.1093/nar/15.24.10587;
RA Ray J., Bird C.R., Maunders M., Grierson D., Schuch W.;
RT "Sequence of pTOM5, a ripening related cDNA from tomato.";
RL Nucleic Acids Res. 15:10587-10587(1987).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Ailsa Craig; TISSUE=Leaf;
RX PubMed=1623189; DOI=10.1007/bf00023387;
RA Ray J., Moureau P., Bird C., Bird A., Grierson D., Maunders M.,
RA Truesdale M., Bramley P., Schuch W.;
RT "Cloning and characterization of a gene involved in phytoene synthesis from
RT tomato.";
RL Plant Mol. Biol. 19:401-404(1992).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA], AND MUTANT.
RC STRAIN=cv. Ailsa Craig; TISSUE=Pericarp;
RX PubMed=8343597; DOI=10.1007/bf00047400;
RA Fray R.G., Grierson D.;
RT "Identification and genetic analysis of normal and mutant phytoene synthase
RT genes of tomato by sequencing, complementation and co-suppression.";
RL Plant Mol. Biol. 22:589-602(1993).
RN [6]
RP INTERACTION WITH SGR1.
RX PubMed=23406468; DOI=10.1111/nph.12175;
RA Luo Z., Zhang J., Li J., Yang C., Wang T., Ouyang B., Li H., Giovannoni J.,
RA Ye Z.;
RT "A STAY-GREEN protein SlSGR1 regulates lycopene and beta-carotene
RT accumulation by interacting directly with SlPSY1 during ripening processes
RT in tomato.";
RL New Phytol. 198:442-452(2013).
CC -!- FUNCTION: Catalyzes the reaction from prephytoene diphosphate to
CC phytoene.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 (2E,6E,10E)-geranylgeranyl diphosphate = 15-cis-phytoene + 2
CC diphosphate; Xref=Rhea:RHEA:34475, ChEBI:CHEBI:27787,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:58756; EC=2.5.1.32;
CC -!- PATHWAY: Carotenoid biosynthesis; phytoene biosynthesis; all-trans-
CC phytoene from geranylgeranyl diphosphate: step 1/1.
CC -!- SUBUNIT: Monomer (By similarity). Interacts with SGR1
CC (PubMed:23406468). {ECO:0000250|UniProtKB:P37272,
CC ECO:0000269|PubMed:23406468}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC -!- DEVELOPMENTAL STAGE: In seedlings and in late stages of fruit ripening.
CC -!- INDUCTION: By fruit ripening.
CC -!- SIMILARITY: Belongs to the phytoene/squalene synthase family.
CC {ECO:0000305}.
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DR EMBL; M84744; AAA34153.1; -; mRNA.
DR EMBL; Y00521; CAA68575.1; -; mRNA.
DR EMBL; X60441; CAA42969.1; -; Genomic_DNA.
DR EMBL; X67144; CAA47625.1; -; mRNA.
DR PIR; A42102; A42102.
DR PIR; S22474; S22474.
DR RefSeq; NP_001234812.1; NM_001247883.2.
DR RefSeq; NP_001334763.1; NM_001347834.1.
DR RefSeq; NP_001334765.1; NM_001347836.1.
DR RefSeq; NP_001334767.1; NM_001347838.1.
DR RefSeq; XP_010317654.1; XM_010319352.2.
DR AlphaFoldDB; P08196; -.
DR SMR; P08196; -.
DR STRING; 4081.Solyc03g031860.2.1; -.
DR PaxDb; P08196; -.
DR PRIDE; P08196; -.
DR EnsemblPlants; Solyc03g031860.3.1; Solyc03g031860.3.1; Solyc03g031860.3.
DR GeneID; 543988; -.
DR Gramene; Solyc03g031860.3.1; Solyc03g031860.3.1; Solyc03g031860.3.
DR KEGG; sly:543988; -.
DR eggNOG; KOG1459; Eukaryota.
DR HOGENOM; CLU_037269_2_0_1; -.
DR InParanoid; P08196; -.
DR OMA; WLLYAWC; -.
DR OrthoDB; 1463212at2759; -.
DR PhylomeDB; P08196; -.
DR BRENDA; 2.5.1.32; 3101.
DR SABIO-RK; P08196; -.
DR UniPathway; UPA00799; UER00773.
DR Proteomes; UP000004994; Chromosome 3.
DR ExpressionAtlas; P08196; baseline.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0004311; F:farnesyltranstransferase activity; IEA:InterPro.
DR GO; GO:0016767; F:geranylgeranyl-diphosphate geranylgeranyltransferase activity; IBA:GO_Central.
DR GO; GO:0016117; P:carotenoid biosynthetic process; IBA:GO_Central.
DR CDD; cd00683; Trans_IPPS_HH; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR002060; Squ/phyt_synthse.
DR InterPro; IPR019845; Squalene/phytoene_synthase_CS.
DR InterPro; IPR044843; Trans_IPPS_bact-type.
DR InterPro; IPR033904; Trans_IPPS_HH.
DR Pfam; PF00494; SQS_PSY; 1.
DR SFLD; SFLDG01212; Phytoene_synthase_like; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
DR PROSITE; PS01044; SQUALEN_PHYTOEN_SYN_1; 1.
DR PROSITE; PS01045; SQUALEN_PHYTOEN_SYN_2; 1.
PE 1: Evidence at protein level;
KW Carotenoid biosynthesis; Chloroplast; Isoprene biosynthesis; Plastid;
KW Reference proteome; Transferase; Transit peptide.
FT TRANSIT 1..129
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 130..412
FT /note="Phytoene synthase 1, chloroplastic"
FT /id="PRO_0000029856"
FT MUTAGEN 388..412
FT /note="KKLIALPIAYAKSLVPPTKTASLQR->NMLKDFFSNFKGSKRGSNATTTLVG
FT : Lack of carotenoids in fruits."
FT CONFLICT 389..407
FT /note="KLIALPIAYAKSLVPPTKT -> QVDCITYCICKISCASYKN (in Ref.
FT 3; CAA68575)"
FT /evidence="ECO:0000305"
FT CONFLICT 389..405
FT /note="KLIALPIAYAKSLVPPT -> QVDCITYCICKISCASY (in Ref. 4;
FT CAA47625)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 412 AA; 46615 MW; DBED05945D6F4C9A CRC64;
MSVALLWVVS PCDVSNGTSF MESVREGNRF FDSSRHRNLV SNERINRGGG KQTNNGRKFS
VRSAILATPS GERTMTSEQM VYDVVLRQAA LVKRQLRSTN ELEVKPDIPI PGNLGLLSEA
YDRCGEVCAE YAKTFNLGTM LMTPERRRAI WAIYVWCRRT DELVDGPNAS YITPAALDRW
ENRLEDVFNG RPFDMLDGAL SDTVSNFPVD IQPFRDMIEG MRMDLRKSRY KNFDELYLYC
YYVAGTVGLM SVPIMGIAPE SKATTESVYN AALALGIANQ LTNILRDVGE DARRGRVYLP
QDELAQAGLS DEDIFAGRVT DKWRIFMKKQ IHRARKFFDE AEKGVTELSS ASRFPVWASL
VLYRKILDEI EANDYNNFTK RAYVSKSKKL IALPIAYAKS LVPPTKTASL QR
