PSY2_ORYSJ
ID PSY2_ORYSJ Reviewed; 398 AA.
AC Q2QLV9; Q8W014;
DT 12-SEP-2018, integrated into UniProtKB/Swiss-Prot.
DT 24-JAN-2006, sequence version 1.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=Phytoene synthase 2, chloroplastic {ECO:0000303|PubMed:15247400};
DE Short=OsPSY2 {ECO:0000303|PubMed:18326788};
DE EC=2.5.1.32 {ECO:0000269|PubMed:15247400};
DE Flags: Precursor;
GN Name=PSY2 {ECO:0000303|PubMed:15247400};
GN OrderedLocusNames=Os12g0626400 {ECO:0000312|EMBL:BAT18187.1},
GN LOC_Os12g43130 {ECO:0000312|EMBL:ABA99494.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, CATALYTIC ACTIVITY, AND TISSUE
RP SPECIFICITY.
RX PubMed=15247400; DOI=10.1104/pp.104.039818;
RA Gallagher C.E., Matthews P.D., Li F., Wurtzel E.T.;
RT "Gene duplication in the carotenoid biosynthetic pathway preceded evolution
RT of the grasses.";
RL Plant Physiol. 135:1776-1783(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16188032; DOI=10.1186/1741-7007-3-20;
RG The rice chromosomes 11 and 12 sequencing consortia;
RT "The sequence of rice chromosomes 11 and 12, rich in disease resistance
RT genes and recent gene duplications.";
RL BMC Biol. 3:20-20(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [7]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=18326788; DOI=10.1104/pp.108.117028;
RA Welsch R., Wuest F., Baer C., Al-Babili S., Beyer P.;
RT "A third phytoene synthase is devoted to abiotic stress-induced abscisic
RT acid formation in rice and defines functional diversification of phytoene
RT synthase genes.";
RL Plant Physiol. 147:367-380(2008).
RN [8]
RP SUBCELLULAR LOCATION.
RX PubMed=23023170; DOI=10.1105/tpc.112.104174;
RA Shumskaya M., Bradbury L.M., Monaco R.R., Wurtzel E.T.;
RT "Plastid localization of the key carotenoid enzyme phytoene synthase is
RT altered by isozyme, allelic variation, and activity.";
RL Plant Cell 24:3725-3741(2012).
RN [9]
RP SUBCELLULAR LOCATION.
RX PubMed=28025520; DOI=10.3390/ijms18010018;
RA You M.K., Kim J.H., Lee Y.J., Jeong Y.S., Ha S.H.;
RT "Plastoglobule-Targeting Competence of a Putative Transit Peptide Sequence
RT from Rice Phytoene Synthase 2 in Plastids.";
RL Int. J. Mol. Sci. 18:0-0(2016).
CC -!- FUNCTION: Catalyzes the conversion of geranylgeranyl diphosphate to
CC phytoene. Mediates the first committed step in carotenoid biosynthesis.
CC {ECO:0000269|PubMed:15247400}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 (2E,6E,10E)-geranylgeranyl diphosphate = 15-cis-phytoene + 2
CC diphosphate; Xref=Rhea:RHEA:34475, ChEBI:CHEBI:27787,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:58756; EC=2.5.1.32;
CC Evidence={ECO:0000269|PubMed:15247400};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane
CC {ECO:0000269|PubMed:18326788}; Peripheral membrane protein
CC {ECO:0000305}. Plastid, chloroplast, plastoglobule
CC {ECO:0000269|PubMed:23023170, ECO:0000269|PubMed:28025520}.
CC -!- TISSUE SPECIFICITY: Expressed in leaves and endosperm
CC (PubMed:15247400). Expressed in developing leaves (PubMed:18326788).
CC {ECO:0000269|PubMed:15247400, ECO:0000269|PubMed:18326788}.
CC -!- INDUCTION: Induced by salt stress and abscisic acid (ABA) in roots.
CC {ECO:0000269|PubMed:15247400}.
CC -!- SIMILARITY: Belongs to the phytoene/squalene synthase family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK07735.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY024351; AAK07735.1; ALT_SEQ; Genomic_DNA.
DR EMBL; DP000011; ABA99494.1; -; Genomic_DNA.
DR EMBL; AP014968; BAT18187.1; -; Genomic_DNA.
DR EMBL; AK073290; BAG93381.1; -; mRNA.
DR RefSeq; XP_015618170.1; XM_015762684.1.
DR AlphaFoldDB; Q2QLV9; -.
DR SMR; Q2QLV9; -.
DR STRING; 4530.OS12T0626400-02; -.
DR PaxDb; Q2QLV9; -.
DR PRIDE; Q2QLV9; -.
DR EnsemblPlants; Os12t0626400-02; Os12t0626400-02; Os12g0626400.
DR GeneID; 4352846; -.
DR Gramene; Os12t0626400-02; Os12t0626400-02; Os12g0626400.
DR KEGG; osa:4352846; -.
DR eggNOG; KOG1459; Eukaryota.
DR HOGENOM; CLU_037269_2_0_1; -.
DR OMA; KLYCYRV; -.
DR OrthoDB; 1463212at2759; -.
DR Proteomes; UP000059680; Chromosome 12.
DR ExpressionAtlas; Q2QLV9; baseline and differential.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0010287; C:plastoglobule; IDA:UniProtKB.
DR GO; GO:0046905; F:15-cis-phytoene synthase activity; IDA:UniProtKB.
DR GO; GO:0004311; F:farnesyltranstransferase activity; IEA:InterPro.
DR GO; GO:0016767; F:geranylgeranyl-diphosphate geranylgeranyltransferase activity; IDA:UniProtKB.
DR GO; GO:0016117; P:carotenoid biosynthetic process; IDA:UniProtKB.
DR CDD; cd00683; Trans_IPPS_HH; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR002060; Squ/phyt_synthse.
DR InterPro; IPR019845; Squalene/phytoene_synthase_CS.
DR InterPro; IPR044843; Trans_IPPS_bact-type.
DR InterPro; IPR033904; Trans_IPPS_HH.
DR Pfam; PF00494; SQS_PSY; 1.
DR SFLD; SFLDG01212; Phytoene_synthase_like; 1.
DR SFLD; SFLDG01018; Squalene/Phytoene_Synthase_Lik; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
DR PROSITE; PS01045; SQUALEN_PHYTOEN_SYN_2; 1.
PE 1: Evidence at protein level;
KW Carotenoid biosynthesis; Chloroplast; Isoprene biosynthesis; Membrane;
KW Plastid; Reference proteome; Transferase; Transit peptide.
FT TRANSIT 1..80
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 81..398
FT /note="Phytoene synthase 2, chloroplastic"
FT /id="PRO_0000444953"
SQ SEQUENCE 398 AA; 44729 MW; 51EE16AA77AE6DB8 CRC64;
MASSSSAAAL WTAAPHPHGS CIRIHAIFHQ RHQRRGRRPV VVASSVRPLQ AASLAVATAP
VAVASRRTAA EEAVYEVVLR QAALVEEATH RRGAGAPRWA EEDAVDWGLL LGDAYHRCGE
VCAEYAKTFY LGTQLMTPER RKAVWAIYVW CRRTDELVDG PNSSYITPKA LDRWEKRLED
LFEGRPYDMY DAALSDTVSK FPVDIQPFKD MIEGMRLDLW KSRYRSFDEL YLYCYYVAGT
VGLMTVPVMG IAPDSKASTE SVYNAALALG IANQLTNILR DVGEDSRRGR IYLPLDELAE
AGLTEEDIFR GKVTDKWRKF MKGQILRARL FFDEAEKGVA HLDSASRWPV LASLWLYRQI
LDAIEANDYN NFTKRAYVNK AKKLLSLPVA YARAAVAS
