PSY3_MAIZE
ID PSY3_MAIZE Reviewed; 426 AA.
AC B0KZ40; B0KYU8; B6SZB8;
DT 12-SEP-2018, integrated into UniProtKB/Swiss-Prot.
DT 18-MAR-2008, sequence version 1.
DT 25-MAY-2022, entry version 45.
DE RecName: Full=Phytoene synthase 3, chloroplastic {ECO:0000303|PubMed:18162592};
DE Short=ZmPSY3 {ECO:0000303|PubMed:18162592};
DE EC=2.5.1.32 {ECO:0000269|PubMed:18162592};
DE Flags: Precursor;
GN Name=PSY3 {ECO:0000303|PubMed:18162592};
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX NCBI_TaxID=4577;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA], FUNCTION, CATALYTIC ACTIVITY,
RP TISSUE SPECIFICITY, AND INDUCTION.
RC STRAIN=cv. B73;
RX PubMed=18162592; DOI=10.1104/pp.107.111120;
RA Li F., Vallabhaneni R., Wurtzel E.T.;
RT "PSY3, a new member of the phytoene synthase gene family conserved in the
RT Poaceae and regulator of abiotic stress-induced root carotenogenesis.";
RL Plant Physiol. 146:1333-1345(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=18937034; DOI=10.1007/s11103-008-9415-4;
RA Alexandrov N.N., Brover V.V., Freidin S., Troukhan M.E., Tatarinova T.V.,
RA Zhang H., Swaller T.J., Lu Y.-P., Bouck J., Flavell R.B., Feldmann K.A.;
RT "Insights into corn genes derived from large-scale cDNA sequencing.";
RL Plant Mol. Biol. 69:179-194(2009).
RN [3]
RP SUBCELLULAR LOCATION.
RX PubMed=23023170; DOI=10.1105/tpc.112.104174;
RA Shumskaya M., Bradbury L.M., Monaco R.R., Wurtzel E.T.;
RT "Plastid localization of the key carotenoid enzyme phytoene synthase is
RT altered by isozyme, allelic variation, and activity.";
RL Plant Cell 24:3725-3741(2012).
CC -!- FUNCTION: Catalyzes the conversion of geranylgeranyl diphosphate to
CC phytoene. Mediates the first committed step in carotenoid biosynthesis.
CC May play a role in regulating carotenoid flux in response to abiotic
CC stress in roots. May control flux to carotenoid precursors that are
CC required for abiotic stress-induced abscisic acid (ABA) formation in
CC roots. {ECO:0000269|PubMed:18162592}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 (2E,6E,10E)-geranylgeranyl diphosphate = 15-cis-phytoene + 2
CC diphosphate; Xref=Rhea:RHEA:34475, ChEBI:CHEBI:27787,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:58756; EC=2.5.1.32;
CC Evidence={ECO:0000269|PubMed:18162592};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast, plastoglobule
CC {ECO:0000269|PubMed:23023170}.
CC -!- TISSUE SPECIFICITY: Expressed in roots and endosperm.
CC {ECO:0000269|PubMed:18162592}.
CC -!- INDUCTION: Induced by drought stress, salt stress and abscisic acid
CC (ABA) in roots. {ECO:0000269|PubMed:18162592}.
CC -!- SIMILARITY: Belongs to the phytoene/squalene synthase family.
CC {ECO:0000305}.
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DR EMBL; DQ372936; ABD17618.1; -; Genomic_DNA.
DR EMBL; DQ356430; ABC75827.1; -; mRNA.
DR EMBL; EU958083; ACG30201.1; -; mRNA.
DR AlphaFoldDB; B0KZ40; -.
DR SMR; B0KZ40; -.
DR STRING; 4577.GRMZM2G300238_P01; -.
DR EnsemblPlants; Zm00001eb320650_T001; Zm00001eb320650_P001; Zm00001eb320650.
DR Gramene; Zm00001eb320650_T001; Zm00001eb320650_P001; Zm00001eb320650.
DR OrthoDB; 1463212at2759; -.
DR Proteomes; UP000007305; Chromosome 7.
DR ExpressionAtlas; B0KZ40; baseline and differential.
DR GO; GO:0010287; C:plastoglobule; IDA:UniProtKB.
DR GO; GO:0046905; F:15-cis-phytoene synthase activity; IDA:UniProtKB.
DR GO; GO:0004311; F:farnesyltranstransferase activity; IEA:InterPro.
DR GO; GO:0016767; F:geranylgeranyl-diphosphate geranylgeranyltransferase activity; IDA:UniProtKB.
DR GO; GO:0016117; P:carotenoid biosynthetic process; IDA:UniProtKB.
DR CDD; cd00683; Trans_IPPS_HH; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR002060; Squ/phyt_synthse.
DR InterPro; IPR019845; Squalene/phytoene_synthase_CS.
DR InterPro; IPR044843; Trans_IPPS_bact-type.
DR InterPro; IPR033904; Trans_IPPS_HH.
DR Pfam; PF00494; SQS_PSY; 1.
DR SFLD; SFLDG01212; Phytoene_synthase_like; 1.
DR SFLD; SFLDG01018; Squalene/Phytoene_Synthase_Lik; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
DR PROSITE; PS01044; SQUALEN_PHYTOEN_SYN_1; 1.
DR PROSITE; PS01045; SQUALEN_PHYTOEN_SYN_2; 1.
PE 1: Evidence at protein level;
KW Carotenoid biosynthesis; Chloroplast; Isoprene biosynthesis; Plastid;
KW Reference proteome; Transferase; Transit peptide.
FT TRANSIT 1..52
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 53..426
FT /note="Phytoene synthase 3, chloroplastic"
FT /id="PRO_0000444951"
FT CONFLICT 59
FT /note="G -> V (in Ref. 1; ABC75827)"
FT /evidence="ECO:0000305"
FT CONFLICT 154
FT /note="M -> I (in Ref. 1; ABC75827)"
FT /evidence="ECO:0000305"
FT CONFLICT 229
FT /note="M -> V (in Ref. 2; ACG30201)"
FT /evidence="ECO:0000305"
FT CONFLICT 382
FT /note="D -> Y (in Ref. 2; ACG30201)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 426 AA; 47301 MW; 4F43F66E2321F93F CRC64;
MMSTSRAVKS PACAARRRQW SADAPNRTAT FLACRHGRRL GGGGGAPCSV RAEGSNTIGC
LEAEAWGGAP ALPGLRVAAP SPGDAFVVPS EQRVHEVVLR QAALAAAAPR TARIEPVPLD
GGLKAAFHRC GEVCREYAKT FYLATQLMTP ERRMAIWAIY VWCRRTDELV DGPNASHISA
LALDRWESRL EDIFAGRPYD MLDAALSDTV ARFPVDIQPF RDMIEGMRMD LKKSRYRSFD
ELYLYCYYVA GTVGLMSVPV MGISPASRAA TETVYKGALA LGLANQLTNI LRDVGEDARR
GRIYLPQDEL EMAGLSDADV LDGRVTDEWR GFMRGQIARA RAFFRQAEEG ATELNQESRW
PVWSSLLLYR QILDEIEAND YDNFTRRAYV PKTKKLMALP KAYLRSLVVP SSSSQAESRR
RYSTLT
