PSY3_ORYSJ
ID PSY3_ORYSJ Reviewed; 444 AA.
AC B6UV92; A0A0P0XQQ5; Q0IZQ9;
DT 12-SEP-2018, integrated into UniProtKB/Swiss-Prot.
DT 16-DEC-2008, sequence version 1.
DT 25-MAY-2022, entry version 68.
DE RecName: Full=Phytoene synthase 3, chloroplastic {ECO:0000303|PubMed:18326788};
DE Short=OsPSY3 {ECO:0000303|PubMed:18326788};
DE EC=2.5.1.32 {ECO:0000269|PubMed:18326788};
DE Flags: Precursor;
GN Name=PSY3 {ECO:0000303|PubMed:18326788};
GN OrderedLocusNames=Os09g0555500 {ECO:0000312|EMBL:BAT09347.1},
GN LOC_Os09g38320 {ECO:0000305};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, SUBCELLULAR
RP LOCATION, TISSUE SPECIFICITY, AND INDUCTION.
RC STRAIN=cv. Taipei 309;
RX PubMed=18326788; DOI=10.1104/pp.108.117028;
RA Welsch R., Wuest F., Baer C., Al-Babili S., Beyer P.;
RT "A third phytoene synthase is devoted to abiotic stress-induced abscisic
RT acid formation in rice and defines functional diversification of phytoene
RT synthase genes.";
RL Plant Physiol. 147:367-380(2008).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=23023170; DOI=10.1105/tpc.112.104174;
RA Shumskaya M., Bradbury L.M., Monaco R.R., Wurtzel E.T.;
RT "Plastid localization of the key carotenoid enzyme phytoene synthase is
RT altered by isozyme, allelic variation, and activity.";
RL Plant Cell 24:3725-3741(2012).
CC -!- FUNCTION: Catalyzes the conversion of geranylgeranyl diphosphate to
CC phytoene. Mediates the first committed step in carotenoid biosynthesis.
CC May play a role in regulating carotenoid flux in response to abiotic
CC stress in roots. May control flux to carotenoid precursors that are
CC required for abiotic stress-induced abscisic acid (ABA) formation in
CC roots. {ECO:0000269|PubMed:18326788}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 (2E,6E,10E)-geranylgeranyl diphosphate = 15-cis-phytoene + 2
CC diphosphate; Xref=Rhea:RHEA:34475, ChEBI:CHEBI:27787,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:58756; EC=2.5.1.32;
CC Evidence={ECO:0000269|PubMed:18326788};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast membrane
CC {ECO:0000269|PubMed:18326788}; Peripheral membrane protein
CC {ECO:0000305}. Plastid, chloroplast, plastoglobule
CC {ECO:0000269|PubMed:23023170}.
CC -!- TISSUE SPECIFICITY: Expressed at low levels in roots and leaves.
CC {ECO:0000269|PubMed:18326788}.
CC -!- INDUCTION: Induced by drought stress, salt stress and abscisic acid
CC (ABA) in roots. {ECO:0000269|PubMed:18326788}.
CC -!- SIMILARITY: Belongs to the phytoene/squalene synthase family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF25806.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAT09347.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; FJ214953; ACI62767.1; -; mRNA.
DR EMBL; AP008215; BAF25806.1; ALT_INIT; Genomic_DNA.
DR EMBL; AP014965; BAT09347.1; ALT_INIT; Genomic_DNA.
DR RefSeq; XP_015611707.1; XM_015756221.1.
DR AlphaFoldDB; B6UV92; -.
DR SMR; B6UV92; -.
DR STRING; 4530.OS09T0555500-01; -.
DR GeneID; 4347813; -.
DR KEGG; osa:4347813; -.
DR eggNOG; KOG1459; Eukaryota.
DR HOGENOM; CLU_037269_2_0_1; -.
DR OrthoDB; 1463212at2759; -.
DR Proteomes; UP000000763; Chromosome 9.
DR Proteomes; UP000059680; Chromosome 9.
DR GO; GO:0031969; C:chloroplast membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0010287; C:plastoglobule; IDA:UniProtKB.
DR GO; GO:0046905; F:15-cis-phytoene synthase activity; IDA:UniProtKB.
DR GO; GO:0004311; F:farnesyltranstransferase activity; IEA:InterPro.
DR GO; GO:0016767; F:geranylgeranyl-diphosphate geranylgeranyltransferase activity; IDA:UniProtKB.
DR GO; GO:0016117; P:carotenoid biosynthetic process; IDA:UniProtKB.
DR CDD; cd00683; Trans_IPPS_HH; 1.
DR Gene3D; 1.10.600.10; -; 1.
DR InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR InterPro; IPR002060; Squ/phyt_synthse.
DR InterPro; IPR019845; Squalene/phytoene_synthase_CS.
DR InterPro; IPR044843; Trans_IPPS_bact-type.
DR InterPro; IPR033904; Trans_IPPS_HH.
DR Pfam; PF00494; SQS_PSY; 1.
DR SFLD; SFLDG01212; Phytoene_synthase_like; 1.
DR SFLD; SFLDG01018; Squalene/Phytoene_Synthase_Lik; 1.
DR SUPFAM; SSF48576; SSF48576; 1.
DR PROSITE; PS01045; SQUALEN_PHYTOEN_SYN_2; 1.
PE 1: Evidence at protein level;
KW Carotenoid biosynthesis; Chloroplast; Isoprene biosynthesis; Membrane;
KW Plastid; Reference proteome; Transferase; Transit peptide.
FT TRANSIT 1..54
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 55..444
FT /note="Phytoene synthase 3, chloroplastic"
FT /id="PRO_0000444954"
SQ SEQUENCE 444 AA; 49367 MW; 174D8FE6C60565E2 CRC64;
MMSTTTTSSA AGSPVCARRR QRVFVDVPRR RATSLARVEY AKMAPPPPPP CSVRAAGSNP
IGCLEVAEPW SGAAPPPLPP LPGHLHVAAP AAEDDDDALA AAAAAVPSEQ RVHDVVLKQA
ALAAAAPEMR RPAQLAERER VAGGLNAAFD RCGEVCKEYA KTFYLATQLM TPERRRAIWA
IYVWCRRTDE LVDGPNASHM SALALDRWES RLDDIFAGRP YDMLDAALSH TVATFPVDIQ
PFRDMIEGMR LDLTKSRYRS FDELYLYCYY VAGTVGLMTV PVMGISPDSR ANTETVYKGA
LALGLANQLT NILRDVGEDA RRGRIYLPMD ELEMAGLSED DIFDGRVTDR WRCFMRDQIT
RARAFFRQAE EGASELNQES RWPVWASLLL YRQILDEIEA NDYNNFTKRA YVPKAKKIVA
LPKAYYRSLM LPSSVRHCSS LTSS
