ID   PSY_CUCME               Reviewed;         422 AA.
AC   P49293;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   03-AUG-2022, entry version 89.
DE   RecName: Full=Phytoene synthase, chloroplastic;
DE            EC=2.5.1.32;
DE   AltName: Full=MEL5;
DE   Flags: Precursor;
GN   Name=PSY;
OS   Cucumis melo (Muskmelon).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Cucurbitales; Cucurbitaceae; Benincaseae; Cucumis.
OX   NCBI_TaxID=3656;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Cantaloup Charentais; TISSUE=Pericarp;
RX   PubMed=7766896; DOI=10.1007/bf00020888;
RA   Karvouni Z., John I., Taylor J.E., Watson C.F., Turner A.J., Grierson D.;
RT   "Isolation and characterisation of a melon cDNA clone encoding phytoene
RT   synthase.";
RL   Plant Mol. Biol. 27:1153-1162(1995).
CC   -!- FUNCTION: Catalyzes the reaction from prephytoene diphosphate to
CC       phytoene.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 (2E,6E,10E)-geranylgeranyl diphosphate = 15-cis-phytoene + 2
CC         diphosphate; Xref=Rhea:RHEA:34475, ChEBI:CHEBI:27787,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:58756; EC=2.5.1.32;
CC   -!- PATHWAY: Carotenoid biosynthesis; phytoene biosynthesis; all-trans-
CC       phytoene from geranylgeranyl diphosphate: step 1/1.
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- SIMILARITY: Belongs to the phytoene/squalene synthase family.
CC       {ECO:0000305}.
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DR   EMBL; Z37543; CAA85775.1; -; mRNA.
DR   PIR; S56668; S56668.
DR   AlphaFoldDB; P49293; -.
DR   SMR; P49293; -.
DR   eggNOG; KOG1459; Eukaryota.
DR   UniPathway; UPA00799; UER00773.
DR   Proteomes; UP000089565; Unplaced.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0004311; F:farnesyltranstransferase activity; IEA:InterPro.
DR   GO; GO:0016767; F:geranylgeranyl-diphosphate geranylgeranyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR   CDD; cd00683; Trans_IPPS_HH; 1.
DR   Gene3D; 1.10.600.10; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR002060; Squ/phyt_synthse.
DR   InterPro; IPR019845; Squalene/phytoene_synthase_CS.
DR   InterPro; IPR044843; Trans_IPPS_bact-type.
DR   InterPro; IPR033904; Trans_IPPS_HH.
DR   Pfam; PF00494; SQS_PSY; 1.
DR   SFLD; SFLDG01212; Phytoene_synthase_like; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
DR   PROSITE; PS01044; SQUALEN_PHYTOEN_SYN_1; 1.
DR   PROSITE; PS01045; SQUALEN_PHYTOEN_SYN_2; 1.
PE   2: Evidence at transcript level;
KW   Carotenoid biosynthesis; Chloroplast; Isoprene biosynthesis; Plastid;
KW   Reference proteome; Transferase; Transit peptide.
FT   TRANSIT         1..83
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           84..422
FT                   /note="Phytoene synthase, chloroplastic"
FT                   /id="PRO_0000029854"
SQ   SEQUENCE   422 AA;  47392 MW;  259124B3AD4642B0 CRC64;
     MSLASSLVVS SNVELSPSSF GFLDSVRDGP QIPDSFRFSS RNRVPNLINK KQKWGNHSHS
     TELKYPILHE SGYGSVIVAS MVANPAGEIA VSAEQKVYNV VMKQAALVKR QLRTAGELDV
     KPDIVLPGTL SLLNEAYDRC GEVCAEYAKT FYLGTMLMTP ERQKAIWAIY VWCRRTDELV
     DGPNASHITP TALDRWEARL EELFQGRPFD MLDAALADTV TKFPVDIQPF KDMIEGMRMD
     LRKSRYKNFD ELYLYCYYVA GTVGLMSVPV MGIAPESQAS TESVYNAALA LGIANQAPPN
     ILRDVGEDAR RGRIYLPQDE LAQAGLSDED IFAGRVTDKW RNFMKNQIKR ARMFFDEAEK
     GVLELNKASR WPVWASLLLY RQILDEIEAN DYDNFTKRAY VSKAKKILAL PMAYGRALLG
     PS