ID   PSY_ONCHC               Reviewed;         412 AA.
AC   Q52QW5;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 49.
DE   RecName: Full=Phytoene synthase, chloroplastic;
DE            Short=OgPSY;
DE            EC=2.5.1.32;
DE   Flags: Precursor;
GN   Name=PSY;
OS   Oncidium hybrid cultivar (Orchid).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Asparagales; Orchidaceae;
OC   Epidendroideae; Cymbidieae; Oncidiinae; Oncidium.
OX   NCBI_TaxID=141207;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX   PubMed=16151849; DOI=10.1007/s00425-005-0113-z;
RA   Hieber A.D., Mudalige-Jayawickrama R.G., Kuehnle A.R.;
RT   "Color genes in the orchid Oncidium Gower Ramsey: identification,
RT   expression, and potential genetic instability in an interspecific cross.";
RL   Planta 223:521-531(2006).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RX   PubMed=20635095; DOI=10.1007/s00425-010-1222-x;
RA   Chiou C.Y., Pan H.A., Chuang Y.N., Yeh K.W.;
RT   "Differential expression of carotenoid-related genes determines diversified
RT   carotenoid coloration in floral tissues of Oncidium cultivars.";
RL   Planta 232:937-948(2010).
CC   -!- FUNCTION: Catalyzes the reaction from prephytoene diphosphate to
CC       phytoene. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 (2E,6E,10E)-geranylgeranyl diphosphate = 15-cis-phytoene + 2
CC         diphosphate; Xref=Rhea:RHEA:34475, ChEBI:CHEBI:27787,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:58756; EC=2.5.1.32;
CC   -!- PATHWAY: Carotenoid biosynthesis; phytoene biosynthesis; all-trans-
CC       phytoene from geranylgeranyl diphosphate: step 1/1.
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC   -!- TISSUE SPECIFICITY: Expressed in roots, leaves, flower buds, sepals,
CC       petals, lips and lip crests. {ECO:0000269|PubMed:16151849,
CC       ECO:0000269|PubMed:20635095}.
CC   -!- DEVELOPMENTAL STAGE: Expressed during floral development.
CC       {ECO:0000269|PubMed:20635095}.
CC   -!- SIMILARITY: Belongs to the phytoene/squalene synthase family.
CC       {ECO:0000305}.
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DR   EMBL; AY973631; AAX84686.1; -; mRNA.
DR   EMBL; FJ859988; ACP27623.1; -; mRNA.
DR   AlphaFoldDB; Q52QW5; -.
DR   SMR; Q52QW5; -.
DR   UniPathway; UPA00799; UER00773.
DR   GO; GO:0010287; C:plastoglobule; IEA:UniProt.
DR   GO; GO:0046905; F:15-cis-phytoene synthase activity; IEA:UniProt.
DR   GO; GO:0004311; F:farnesyltranstransferase activity; IEA:InterPro.
DR   GO; GO:0016767; F:geranylgeranyl-diphosphate geranylgeranyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0016117; P:carotenoid biosynthetic process; IEA:UniProtKB-KW.
DR   CDD; cd00683; Trans_IPPS_HH; 1.
DR   Gene3D; 1.10.600.10; -; 1.
DR   InterPro; IPR008949; Isoprenoid_synthase_dom_sf.
DR   InterPro; IPR002060; Squ/phyt_synthse.
DR   InterPro; IPR019845; Squalene/phytoene_synthase_CS.
DR   InterPro; IPR044843; Trans_IPPS_bact-type.
DR   InterPro; IPR033904; Trans_IPPS_HH.
DR   Pfam; PF00494; SQS_PSY; 1.
DR   SFLD; SFLDG01212; Phytoene_synthase_like; 1.
DR   SUPFAM; SSF48576; SSF48576; 1.
DR   PROSITE; PS01044; SQUALEN_PHYTOEN_SYN_1; 1.
DR   PROSITE; PS01045; SQUALEN_PHYTOEN_SYN_2; 1.
PE   2: Evidence at transcript level;
KW   Carotenoid biosynthesis; Chloroplast; Isoprene biosynthesis; Plastid;
KW   Transferase; Transit peptide.
FT   TRANSIT         1..?
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..412
FT                   /note="Phytoene synthase, chloroplastic"
FT                   /id="PRO_0000426707"
SQ   SEQUENCE   412 AA;  47257 MW;  53B4C409EB795C67 CRC64;
     MAASQLCFVG FLEGIRGGDR LWNAKNDCRF SQRKKMRWSF YCLFTNFNYA CVSQEPEKDL
     KFPIYSSLVV NPVGEVAISS EQKVYDVVLK QAALVEQQLR NRTVLEEKTG TTFLLNEAYD
     RCGQICEEYA KTFYLGTLLM TPERRRAIWA IYVWCRRTDE LVDGPNASHI TPSALDRWEA
     RLEDLFAGRP YDMLDASLSD TVVNFPVDIQ PFKDMIEGMR MDLKKSRYKN FDELYLYCYY
     VAGTVGLMSV PVMGIDPESD ATTESVYNAA LSLGIANQLT NILRDVGEDT RRGRVYLPQD
     ELAEAGLSDE DIFNGKVTDR WRNFMKNQIK RARTFFQEAE KGIAELNQAS RWPVLASLLL
     YRQILDEIEA NDYNNFTKRA YVSKAKKLMA VPVAYGRSLI RPSMKKPSLV KP