PT122_YEAST
ID PT122_YEAST Reviewed; 254 AA.
AC P10355; D3DM60;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-1992, sequence version 2.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=Protein PET122, mitochondrial;
DE Flags: Precursor;
GN Name=PET122; OrderedLocusNames=YER153C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204510 / AB320;
RX PubMed=2849752; DOI=10.1093/nar/16.22.10783;
RA Ohmen J.D., Kloeckener-Gruissem B., McEwen J.E.;
RT "Molecular cloning and nucleotide sequence of the nuclear PET122 gene
RT required for expression of the mitochondrial COX3 gene in S. cerevisiae.";
RL Nucleic Acids Res. 16:10783-10802(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SEQUENCE REVISION.
RX PubMed=2160592; DOI=10.1128/mcb.10.6.3027-3035.1990;
RA Ohmen J.D., Burke K.A., McEwen J.E.;
RT "Divergent overlapping transcripts at the PET122 locus in Saccharomyces
RT cerevisiae.";
RL Mol. Cell. Biol. 10:3027-3035(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169868;
RA Dietrich F.S., Mulligan J.T., Hennessy K.M., Yelton M.A., Allen E.,
RA Araujo R., Aviles E., Berno A., Brennan T., Carpenter J., Chen E.,
RA Cherry J.M., Chung E., Duncan M., Guzman E., Hartzell G., Hunicke-Smith S.,
RA Hyman R.W., Kayser A., Komp C., Lashkari D., Lew H., Lin D., Mosedale D.,
RA Nakahara K., Namath A., Norgren R., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Sehl P., Schramm S., Shogren T., Smith V., Taylor P., Wei Y.,
RA Botstein D., Davis R.W.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome V.";
RL Nature 387:78-81(1997).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [6]
RP PROTEIN SEQUENCE OF 9-19, AND SUBCELLULAR LOCATION.
RX PubMed=8389363; DOI=10.1016/s0021-9258(19)50261-7;
RA McMullin T.W., Fox T.D.;
RT "COX3 mRNA-specific translational activator proteins are associated with
RT the inner mitochondrial membrane in Saccharomyces cerevisiae.";
RL J. Biol. Chem. 268:11737-11741(1993).
RN [7]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Required for expression of the mitochondrial gene for
CC cytochrome c oxidase subunit 3 (COX3). PET122 seems to work by directly
CC interacting with the small ribosomal subunit to promote translation
CC initiation on the COX3 mRNA.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:8389363}; Peripheral membrane protein
CC {ECO:0000269|PubMed:8389363}.
CC -!- MISCELLANEOUS: Present with 468 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA30439.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; X07558; CAA30439.1; ALT_FRAME; Genomic_DNA.
DR EMBL; U18917; AAB64680.1; -; Genomic_DNA.
DR EMBL; AY557785; AAS56111.1; -; Genomic_DNA.
DR EMBL; BK006939; DAA07814.1; -; Genomic_DNA.
DR PIR; B36328; BVBY22.
DR RefSeq; NP_011080.1; NM_001179043.1.
DR AlphaFoldDB; P10355; -.
DR BioGRID; 36903; 221.
DR DIP; DIP-5415N; -.
DR IntAct; P10355; 2.
DR MINT; P10355; -.
DR STRING; 4932.YER153C; -.
DR PaxDb; P10355; -.
DR PRIDE; P10355; -.
DR EnsemblFungi; YER153C_mRNA; YER153C; YER153C.
DR GeneID; 856897; -.
DR KEGG; sce:YER153C; -.
DR SGD; S000000955; PET122.
DR VEuPathDB; FungiDB:YER153C; -.
DR eggNOG; ENOG502RYT2; Eukaryota.
DR HOGENOM; CLU_084817_0_0_1; -.
DR InParanoid; P10355; -.
DR OMA; KSCQWGH; -.
DR BioCyc; YEAST:G3O-30314-MON; -.
DR PRO; PR:P10355; -.
DR Proteomes; UP000002311; Chromosome V.
DR RNAct; P10355; protein.
DR GO; GO:0031305; C:integral component of mitochondrial inner membrane; IDA:SGD.
DR GO; GO:0005743; C:mitochondrial inner membrane; IDA:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0003743; F:translation initiation factor activity; IMP:SGD.
DR GO; GO:0033617; P:mitochondrial cytochrome c oxidase assembly; IMP:SGD.
DR GO; GO:0070131; P:positive regulation of mitochondrial translation; IMP:SGD.
DR InterPro; IPR008732; Pet122.
DR Pfam; PF05476; PET122; 1.
DR PIRSF; PIRSF003326; PET122; 1.
PE 1: Evidence at protein level;
KW Activator; Direct protein sequencing; Membrane; Mitochondrion;
KW Mitochondrion inner membrane; Reference proteome; Transit peptide;
KW Translation regulation.
FT TRANSIT 1..8
FT /note="Mitochondrion"
FT /evidence="ECO:0000269|PubMed:8389363"
FT CHAIN 9..254
FT /note="Protein PET122, mitochondrial"
FT /id="PRO_0000022177"
FT REGION 185..254
FT /note="Essential for PET122 function"
SQ SEQUENCE 254 AA; 29155 MW; 321E76E204B93209 CRC64;
MLTITKRLVT TDVRSRILLS SLNGKMSDAL ALLRQQQQTS VDVELLHTML ARAAALAHAD
TIAYMWYQHV MPRRLPVEGR LLCEMAGVAL YQDRLFLPAQ FLQHYQAMNR DRRTSPEDEL
IEYELRRIKV EAFARGTMHS TALREKWKVF LQEMDTLPGQ PPLRLRDFPQ MTKAMGIALM
QQDEQAAALA LFGRQPLVIK NEWSLPLLLA GVLWHVPGPA QARRVLAEFR QSYRGLPLLD
AELVIKRRGF EINT
