PT309_YEAST
ID PT309_YEAST Reviewed; 965 AA.
AC P32522; D6VY68;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Pentatricopeptide repeat-containing protein PET309, mitochondrial;
DE Flags: Precursor;
GN Name=PET309; OrderedLocusNames=YLR067C; ORFNames=L2189;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7664742; DOI=10.1002/j.1460-2075.1995.tb00074.x;
RA Manthey G.M., McEwen J.E.;
RT "The product of the nuclear gene PET309 is required for translation of
RT mature mRNA and stability or production of intron-containing RNAs derived
RT from the mitochondrial COX1 locus of Saccharomyces cerevisiae.";
RL EMBO J. 14:4031-4043(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=9692914; DOI=10.1046/j.1432-1327.1998.2550156.x;
RA Manthey G.M., Przybyla-Zawislak B.D., McEwen J.E.;
RT "The Saccharomyces cerevisiae Pet309 protein is embedded in the
RT mitochondrial inner membrane.";
RL Eur. J. Biochem. 255:156-161(1998).
RN [5]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Required for initiation of translation of the COX1 coding
CC region. Also involved in the stability of the intron containing
CC transcript of COX1.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane {ECO:0000305};
CC Single-pass membrane protein {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 1160 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; L06072; AAA34856.1; -; Genomic_DNA.
DR EMBL; X94607; CAA64313.1; -; Genomic_DNA.
DR EMBL; Z73239; CAA97623.1; -; Genomic_DNA.
DR EMBL; Z73240; CAA97625.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09384.1; -; Genomic_DNA.
DR PIR; S58393; S58393.
DR RefSeq; NP_013168.1; NM_001181954.1.
DR AlphaFoldDB; P32522; -.
DR BioGRID; 31341; 110.
DR DIP; DIP-932N; -.
DR IntAct; P32522; 4.
DR MINT; P32522; -.
DR STRING; 4932.YLR067C; -.
DR iPTMnet; P32522; -.
DR MaxQB; P32522; -.
DR PaxDb; P32522; -.
DR PRIDE; P32522; -.
DR EnsemblFungi; YLR067C_mRNA; YLR067C; YLR067C.
DR GeneID; 850756; -.
DR KEGG; sce:YLR067C; -.
DR SGD; S000004057; PET309.
DR VEuPathDB; FungiDB:YLR067C; -.
DR eggNOG; KOG4197; Eukaryota.
DR GeneTree; ENSGT00390000016775; -.
DR HOGENOM; CLU_306786_0_0_1; -.
DR InParanoid; P32522; -.
DR OMA; YFMKSYL; -.
DR BioCyc; YEAST:G3O-32220-MON; -.
DR ChiTaRS; PET309; yeast.
DR PRO; PR:P32522; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; P32522; protein.
DR GO; GO:0031314; C:extrinsic component of mitochondrial inner membrane; IDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005759; C:mitochondrial matrix; ISS:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0003729; F:mRNA binding; IDA:SGD.
DR GO; GO:0045182; F:translation regulator activity; IMP:SGD.
DR GO; GO:0000049; F:tRNA binding; ISS:UniProtKB.
DR GO; GO:0033617; P:mitochondrial cytochrome c oxidase assembly; IMP:SGD.
DR GO; GO:0070134; P:positive regulation of mitochondrial translational initiation; IMP:SGD.
DR GO; GO:0042780; P:tRNA 3'-end processing; ISS:UniProtKB.
DR Gene3D; 1.25.40.10; -; 2.
DR InterPro; IPR002885; Pentatricopeptide_repeat.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PROSITE; PS51375; PPR; 9.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Repeat; Transit peptide; Transmembrane; Transmembrane helix.
FT TRANSIT 1..?
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN ?..965
FT /note="Pentatricopeptide repeat-containing protein PET309,
FT mitochondrial"
FT /id="PRO_0000022179"
FT TRANSMEM 237..256
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REPEAT 310..344
FT /note="PPR 1"
FT REPEAT 345..379
FT /note="PPR 2"
FT REPEAT 380..414
FT /note="PPR 3"
FT REPEAT 415..450
FT /note="PPR 4"
FT REPEAT 451..486
FT /note="PPR 5"
FT REPEAT 487..523
FT /note="PPR 6"
FT REPEAT 524..558
FT /note="PPR 7"
FT REPEAT 559..595
FT /note="PPR 8"
FT REPEAT 596..630
FT /note="PPR 9"
FT REPEAT 680..714
FT /note="PPR 10"
FT REPEAT 723..763
FT /note="PPR 11"
FT REPEAT 774..808
FT /note="PPR 12"
FT REPEAT 809..844
FT /note="PPR 13"
SQ SEQUENCE 965 AA; 112646 MW; 3E4B016D933AD4C5 CRC64;
MKRCAPAVLR NYNYKKGIWS TGVPDHIRKL LRDKSTSPLC SQDERNLVSY FMARGSVPLK
SVGSGLTKKA TTSITSNSAT TTFERQYLIK YLYRHQAYGN VIKIAQKFLY TTIGSQRLLK
QDASLPELKK FLLSLLILQR GIQLDQAISD IIQRFLLTQK TMVIDLINSI FSRMVIMNMH
EEAVYKWVKW MKLVNGHCEF TNYMENKIVL RNFLSFMRQS NVRPDYLSYL KAIQLTQGPA
IASQFATTLL FLLTYIRKFS SAEAVWNYKC EHNLPIVSSD LTCILKTYCH MQKFNLVSST
YWKYPDAQHD QNQFDYLLVA HSRLHNWDAL QQQFNALFGI GKLPSIQHYG ILMYTMARIG
ELDSVNKLYT QLLRRGMIPT YAVLQSLLYA HYKVGDFAAC FSHFELFKKY DITPSTATHT
IMLKVYRGLN DLDGAFRILK RLSEDPSVEI TEGHFALLIQ MCCKTTNHLI AQELFNLMTE
HYNIQHTGKS ISALMDVYIE SNRPTEAIAL FEKHSKNLSW RDGLISVYNK AIKAYIGLRN
ANKCEELFDK ITTSKLAVNS EFYKMMIKFL VTLNEDCETA LSIIDQLIKH SVIKVDATHF
EIIMEAYDKE GYRDGIINLY KTMSQNKVPA NSKILYYILK AVAKKSLQNN EEIKETINMV
EDIMENAANG TLDVTYNKLH PSVMAWPMRM IVKHDSPQRA LELYNRYNEL FFKKHDWISN
NNKFVMMRSL LVLLAQIEQW KDFETLFAKY MDRIENIENL PSSTTPNIKL RSIFSGLFPY
KVSQLIAMNK IDELPLLWKK LREKGFILDN ISWNSAVEAL FKDPRTLSYG MKIVDDTLIH
GYNLIHKFRL LTKLSEDKTQ SSDKSWPTLK MKEKEPNKFQ PRLYLKSDTY NSIMRQLDTY
LDGIDDLKTL EDQVRDFISN YKYFMKDYLL MPRSKINKWE QIEMRHLSYF KELRKSKRVL
PVSKF
