PTA10_ARATH
ID PTA10_ARATH Reviewed; 668 AA.
AC F4JF21; Q0WNC4; Q8L7P3; Q9M3G9; Q9SMP7;
DT 24-JUN-2015, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 03-AUG-2022, entry version 68.
DE RecName: Full=Protein PLASTID TRANSCRIPTIONALLY ACTIVE 10 {ECO:0000303|PubMed:16326926};
DE Short=pTAC10 {ECO:0000303|PubMed:16326926};
DE AltName: Full=Plastid-encoded RNA polymerase-associated protein 3 {ECO:0000303|PubMed:21949211};
DE Short=PEP-associated protein 3 {ECO:0000303|PubMed:21949211};
DE AltName: Full=Protein PIGMENT DEFECTIVE 312;
DE Flags: Precursor;
GN Name=PTAC10 {ECO:0000303|PubMed:16326926};
GN Synonyms=PAP3 {ECO:0000303|PubMed:21949211}, PDE312,
GN TAC10 {ECO:0000303|PubMed:16326926};
GN OrderedLocusNames=At3g48500 {ECO:0000312|Araport:AT3G48500};
GN ORFNames=T29H11_270 {ECO:0000312|EMBL:CAB88331.1},
GN T8P19.10 {ECO:0000312|EMBL:CAB62339.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 56-668 (ISOFORM 2).
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NOMENCLATURE.
RX PubMed=16326926; DOI=10.1105/tpc.105.036392;
RA Pfalz J., Liere K., Kandlbinder A., Dietz K.-J., Oelmueller R.;
RT "pTAC2, -6, and -12 are components of the transcriptionally active plastid
RT chromosome that are required for plastid gene expression.";
RL Plant Cell 18:176-197(2006).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-434, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19376835; DOI=10.1104/pp.109.138677;
RA Reiland S., Messerli G., Baerenfaller K., Gerrits B., Endler A.,
RA Grossmann J., Gruissem W., Baginsky S.;
RT "Large-scale Arabidopsis phosphoproteome profiling reveals novel
RT chloroplast kinase substrates and phosphorylation networks.";
RL Plant Physiol. 150:889-903(2009).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBUNIT.
RX PubMed=21949211; DOI=10.1104/pp.111.184515;
RA Steiner S., Schroeter Y., Pfalz J., Pfannschmidt T.;
RT "Identification of essential subunits in the plastid-encoded RNA polymerase
RT complex reveals building blocks for proper plastid development.";
RL Plant Physiol. 157:1043-1055(2011).
RN [8]
RP INTERACTION WITH PTAC7.
RX PubMed=23082802; DOI=10.1111/j.1399-3054.2012.01718.x;
RA Yu Q.-B., Lu Y., Ma Q., Zhao T.-T., Huang C., Zhao H.-F., Zhang X.-L.,
RA Lv R.-H., Yang Z.-N.;
RT "TAC7, an essential component of the plastid transcriptionally active
RT chromosome complex, interacts with FLN1, TAC10, TAC12 and TAC14 to regulate
RT chloroplast gene expression in Arabidopsis thaliana.";
RL Physiol. Plantarum 148:408-421(2013).
CC -!- SUBUNIT: Component of the transcriptionally active chromosome (TAC)
CC complexes (PubMed:21949211). Interacts with PTAC7 (PubMed:23082802).
CC {ECO:0000269|PubMed:21949211, ECO:0000269|PubMed:23082802}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=F4JF21-1; Sequence=Displayed;
CC Name=2;
CC IsoId=F4JF21-2; Sequence=VSP_057776;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM91538.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAB62339.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB88331.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AL049659; CAB88331.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL133315; CAB62339.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE78423.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE78424.1; -; Genomic_DNA.
DR EMBL; AK229521; BAF01376.1; -; mRNA.
DR EMBL; AY128335; AAM91538.1; ALT_INIT; mRNA.
DR PIR; T46194; T46194.
DR RefSeq; NP_001078259.1; NM_001084790.1. [F4JF21-2]
DR RefSeq; NP_190419.3; NM_114708.5. [F4JF21-1]
DR AlphaFoldDB; F4JF21; -.
DR STRING; 3702.AT3G48500.2; -.
DR iPTMnet; F4JF21; -.
DR PaxDb; F4JF21; -.
DR PRIDE; F4JF21; -.
DR ProteomicsDB; 248677; -. [F4JF21-1]
DR EnsemblPlants; AT3G48500.1; AT3G48500.1; AT3G48500. [F4JF21-1]
DR EnsemblPlants; AT3G48500.2; AT3G48500.2; AT3G48500. [F4JF21-2]
DR GeneID; 824009; -.
DR Gramene; AT3G48500.1; AT3G48500.1; AT3G48500. [F4JF21-1]
DR Gramene; AT3G48500.2; AT3G48500.2; AT3G48500. [F4JF21-2]
DR KEGG; ath:AT3G48500; -.
DR Araport; AT3G48500; -.
DR TAIR; locus:2101150; AT3G48500.
DR eggNOG; ENOG502QPXQ; Eukaryota.
DR OMA; PPIFHRE; -.
DR OrthoDB; 297641at2759; -.
DR PRO; PR:F4JF21; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; F4JF21; baseline and differential.
DR Genevisible; F4JF21; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0042644; C:chloroplast nucleoid; HDA:TAIR.
DR GO; GO:0009570; C:chloroplast stroma; HDA:TAIR.
DR GO; GO:0000427; C:plastid-encoded plastid RNA polymerase complex; IEA:InterPro.
DR GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR044967; PTAC10.
DR InterPro; IPR003029; S1_domain.
DR PANTHER; PTHR36371; PTHR36371; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR PROSITE; PS50126; S1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Chloroplast; Phosphoprotein; Plastid;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..40
FT /note="Chloroplast"
FT /evidence="ECO:0000305"
FT CHAIN 41..668
FT /note="Protein PLASTID TRANSCRIPTIONALLY ACTIVE 10"
FT /evidence="ECO:0000255"
FT /id="PRO_0000433434"
FT DOMAIN 272..340
FT /note="S1 motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00180"
FT REGION 362..394
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 611..668
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 621..643
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 647..668
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 434
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19376835"
FT VAR_SEQ 325
FT /note="T -> TVILRHIFFFRIYKNLNGNCFILLFLLFFQ (in isoform 2)"
FT /id="VSP_057776"
SQ SEQUENCE 668 AA; 78814 MW; A3B3A2454512C262 CRC64;
MQICQTKLNF TFPNPTNPNF CKPKALQWSP PRRISLLPCR GFSSDEFPVD ETFLEKFGPK
DKDTEDEARR RNWIERGWAP WEEILTPEAD FARKSLNEGE EVPLQSPEAI EAFKMLRPSY
RKKKIKEMGI TEDEWYAKQF EIRGDKPPPL ETSWAGPMVL RQIPPRDWPP RGWEVDRKEL
EFIREAHKLM AERVWLEDLD KDLRVGEDAT VDKMCLERFK VFLKQYKEWV EDNKDRLEEE
SYKLDQDFYP GRRKRGKDYE DGMYELPFYY PGMVCEGTVT TLHLYQGAFV DIGGVHEGWV
PIKGNDWFWI RHFIKVGMHV IVEITAKRDP YRFRFPLELR FVHPNIDHMI FNKFDFPPIF
HRDGDTNPDE IRRDCGRPPE PRKDPGSKPE EEGLLSDHPY VDKLWQIHVA EQMILGDYEA
NPAKYEGKKL SELSDDEDFD EQKDIEYGEA YYKKTKLPKV ILKTSVKELD LEAALTERQH
HNKLMMEAKA RGEGYKIDKL RRNIEMDEYD FLHWRRSLEE REALLRDISS RQALGLPLEE
PGRYKPGSFF GKDQYDPTSA LYQYDYWGEP KNSEISKQER MKDAHNKSIV GKGNVWYDMS
YDDAIKQTIE KRKEGSTLAS QEEETESEEE EEDDDDFDDF DYSILSDESS IGYSEQQPLV
NGTQVLTD
