PTA12_ARATH
ID PTA12_ARATH Reviewed; 527 AA.
AC F4IHY7; O64683; Q8GY76;
DT 24-JUN-2015, integrated into UniProtKB/Swiss-Prot.
DT 28-JUN-2011, sequence version 1.
DT 25-MAY-2022, entry version 59.
DE RecName: Full=Protein PLASTID TRANSCRIPTIONALLY ACTIVE 12, chloroplastic {ECO:0000303|PubMed:16326926};
DE Short=pTAC12 {ECO:0000303|PubMed:16326926};
DE AltName: Full=Plastid-encoded RNA polymerase-associated protein 5 {ECO:0000303|PubMed:21949211};
DE Short=PEP-associated protein 5 {ECO:0000303|PubMed:21949211};
DE AltName: Full=Protein HEMERA {ECO:0000303|PubMed:20603003};
DE Flags: Precursor;
GN Name=PTAC12 {ECO:0000303|PubMed:16326926};
GN Synonyms=HMR {ECO:0000303|PubMed:20603003},
GN PAP5 {ECO:0000303|PubMed:21949211}, TAC12 {ECO:0000303|PubMed:16326926};
GN OrderedLocusNames=At2g34640 {ECO:0000312|Araport:AT2G34640};
GN ORFNames=T31E10.2 {ECO:0000312|EMBL:AAC26686.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP AND NOMENCLATURE.
RX PubMed=16326926; DOI=10.1105/tpc.105.036392;
RA Pfalz J., Liere K., Kandlbinder A., Dietz K.-J., Oelmueller R.;
RT "pTAC2, -6, and -12 are components of the transcriptionally active plastid
RT chromosome that are required for plastid gene expression.";
RL Plant Cell 18:176-197(2006).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP INDUCTION BY LIGHT, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=20603003; DOI=10.1016/j.cell.2010.05.007;
RA Chen M., Galvao R.M., Li M., Burger B., Bugea J., Bolado J., Chory J.;
RT "Arabidopsis HEMERA/pTAC12 initiates photomorphogenesis by phytochromes.";
RL Cell 141:1230-1240(2010).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBUNIT.
RX PubMed=21949211; DOI=10.1104/pp.111.184515;
RA Steiner S., Schroeter Y., Pfalz J., Pfannschmidt T.;
RT "Identification of essential subunits in the plastid-encoded RNA polymerase
RT complex reveals building blocks for proper plastid development.";
RL Plant Physiol. 157:1043-1055(2011).
RN [7]
RP INTERACTION WITH PTAC14.
RC STRAIN=cv. Columbia;
RX PubMed=22010110; DOI=10.1104/pp.111.184762;
RA Gao Z.-P., Yu Q.-B., Zhao T.-T., Ma Q., Chen G.-X., Yang Z.-N.;
RT "A functional component of the transcriptionally active chromosome complex,
RT Arabidopsis pTAC14, interacts with pTAC12/HEMERA and regulates plastid gene
RT expression.";
RL Plant Physiol. 157:1733-1745(2011).
RN [8]
RP FUNCTION, MUTAGENESIS OF ARG-293, INTERACTION WITH PHYA AND PHYB, INDUCTION
RP BY LIGHT, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=22895253; DOI=10.1101/gad.193219.112;
RA Galvao R.M., Li M., Kothadia S.M., Haskel J.D., Decker P.V.,
RA Van Buskirk E.K., Chen M.;
RT "Photoactivated phytochromes interact with HEMERA and promote its
RT accumulation to establish photomorphogenesis in Arabidopsis.";
RL Genes Dev. 26:1851-1863(2012).
RN [9]
RP REVIEW.
RX PubMed=22902688; DOI=10.4161/psb.21618;
RA Gao Z.-P., Chen G.-X., Yang Z.-N.;
RT "Regulatory role of Arabidopsis pTAC14 in chloroplast development and
RT plastid gene expression.";
RL Plant Signal. Behav. 7:1354-1356(2012).
RN [10]
RP INTERACTION WITH PTAC7.
RX PubMed=23082802; DOI=10.1111/j.1399-3054.2012.01718.x;
RA Yu Q.-B., Lu Y., Ma Q., Zhao T.-T., Huang C., Zhao H.-F., Zhang X.-L.,
RA Lv R.-H., Yang Z.-N.;
RT "TAC7, an essential component of the plastid transcriptionally active
RT chromosome complex, interacts with FLN1, TAC10, TAC12 and TAC14 to regulate
RT chloroplast gene expression in Arabidopsis thaliana.";
RL Physiol. Plantarum 148:408-421(2013).
CC -!- FUNCTION: Involved in plastid gene expression (PubMed:16326926).
CC Required in the nucleus for the initiation of photomorphogenesis
CC mediated by phytochromes (PHYs) (e.g. PHYA and PHYB) by mediating PHYs
CC localization to photobodies, especially in response to red and far-red
CC light, and implicating phytochrome nuclear bodies as sites of
CC proteolysis for PHYs and PIFs proteins (e.g. PIF1 and PIF3). Acts
CC downstream of PHYs and upstream of DET1 (PubMed:20603003,
CC PubMed:22895253). {ECO:0000269|PubMed:16326926,
CC ECO:0000269|PubMed:20603003, ECO:0000269|PubMed:22895253}.
CC -!- SUBUNIT: Component of the transcriptionally active chromosome (TAC)
CC complexes (PubMed:21949211, PubMed:22010110). Interacts with PTAC14 and
CC PTAC7 (PubMed:22010110, PubMed:23082802). Binds to photoactivated
CC phytochromes (e.g. PHYA and PHYB) via their photosensory domains; these
CC interactions stimulate its light-mediated accumulation
CC (PubMed:22895253). {ECO:0000269|PubMed:21949211,
CC ECO:0000269|PubMed:22010110, ECO:0000269|PubMed:22895253,
CC ECO:0000269|PubMed:23082802}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:16326926, ECO:0000269|PubMed:20603003}. Nucleus
CC {ECO:0000269|PubMed:20603003, ECO:0000269|PubMed:22895253}.
CC Note=Localizes to subnuclear domains in the nucleolus and in the
CC nucleoplasm, and in chloroplasts. {ECO:0000269|PubMed:20603003}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in cotyledons, leaves, stems and
CC flowers, but barely in roots. {ECO:0000269|PubMed:16326926,
CC ECO:0000269|PubMed:20603003}.
CC -!- DEVELOPMENTAL STAGE: In young seedlings, expressed predominantly in
CC cotyledons, at the top of the hypocotyl, and in the root tip,
CC regardless of light conditions. In older seedlings, confined to
CC unexpanded cotyledons of dark-grown seedlings.
CC {ECO:0000269|PubMed:20603003}.
CC -!- INDUCTION: Regulated by light at the post-translational level;
CC constitutively expressed at transcript level, but accumulates in
CC response to light (e.g. red, far red, blue and white light) at protein
CC level. {ECO:0000269|PubMed:20603003, ECO:0000269|PubMed:22895253}.
CC -!- DISRUPTION PHENOTYPE: Seedling lethal without exogenous carbon sources
CC (PubMed:16326926, PubMed:20603003). Decrease of total chlorophyll
CC content as well as a decrease in the chlorophyll a:b ratio. Absence of
CC grana thylakoids. Altered expression profiles of plastid genes
CC (PubMed:16326926). Impaired in phytochrome (both PHYA and PHYB)
CC responses, especially to red and far-red light, such as hypocotyl
CC growth inhibition, proteolysis of phytochrome A and phytochrome-
CC interacting transcription factors (PubMed:20603003).
CC {ECO:0000269|PubMed:16326926, ECO:0000269|PubMed:20603003}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC26686.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC004077; AAC26686.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC09002.1; -; Genomic_DNA.
DR EMBL; AK117813; BAC42455.2; -; mRNA.
DR PIR; B84759; B84759.
DR RefSeq; NP_181011.2; NM_129018.4.
DR AlphaFoldDB; F4IHY7; -.
DR IntAct; F4IHY7; 1.
DR MINT; F4IHY7; -.
DR STRING; 3702.AT2G34640.1; -.
DR PaxDb; F4IHY7; -.
DR PRIDE; F4IHY7; -.
DR ProteomicsDB; 248842; -.
DR EnsemblPlants; AT2G34640.1; AT2G34640.1; AT2G34640.
DR GeneID; 818029; -.
DR Gramene; AT2G34640.1; AT2G34640.1; AT2G34640.
DR KEGG; ath:AT2G34640; -.
DR Araport; AT2G34640; -.
DR TAIR; locus:2062286; AT2G34640.
DR eggNOG; ENOG502QS8P; Eukaryota.
DR HOGENOM; CLU_037383_0_0_1; -.
DR InParanoid; F4IHY7; -.
DR OMA; YSYMDST; -.
DR OrthoDB; 586120at2759; -.
DR PRO; PR:F4IHY7; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; F4IHY7; baseline and differential.
DR Genevisible; F4IHY7; AT.
DR GO; GO:0009507; C:chloroplast; IDA:UniProtKB.
DR GO; GO:0042644; C:chloroplast nucleoid; HDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0000427; C:plastid-encoded plastid RNA polymerase complex; IDA:UniProtKB.
DR GO; GO:0042793; P:plastid transcription; IMP:TAIR.
DR GO; GO:0090228; P:positive regulation of red or far-red light signaling pathway; IMP:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:TAIR.
DR GO; GO:0009585; P:red, far-red light phototransduction; IEA:UniProtKB-KW.
DR GO; GO:0009637; P:response to blue light; IEP:UniProtKB.
DR GO; GO:0010218; P:response to far red light; IEP:UniProtKB.
DR GO; GO:0009416; P:response to light stimulus; IEP:UniProtKB.
DR GO; GO:0010114; P:response to red light; IEP:UniProtKB.
DR InterPro; IPR034581; PTAC12.
DR PANTHER; PTHR35720; PTHR35720; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Nucleus; Phytochrome signaling pathway; Plastid;
KW Reference proteome; Transcription; Transcription regulation;
KW Transit peptide.
FT TRANSIT 1..30
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 31..527
FT /note="Protein PLASTID TRANSCRIPTIONALLY ACTIVE 12,
FT chloroplastic"
FT /id="PRO_0000433435"
FT REGION 1..115
FT /note="PHYA-interacting region 1 (PIR1)"
FT /evidence="ECO:0000269|PubMed:22895253"
FT REGION 89..188
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 252..352
FT /note="PHYA-interacting region 2 (PIR2)"
FT /evidence="ECO:0000269|PubMed:22895253"
FT REGION 458..527
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 204..211
FT /note="Nuclear localization signal 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT MOTIF 235..242
FT /note="Nuclear localization signal 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00768"
FT COMPBIAS 162..181
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 488..508
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 509..527
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 293
FT /note="R->K: Slightly weaker interaction with PHYA and PHYB
FT and impaired light-mediated accumulation."
FT /evidence="ECO:0000269|PubMed:22895253"
FT CONFLICT 375
FT /note="D -> N (in Ref. 3; BAC42455)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 527 AA; 60895 MW; 0F4AF734815F8C27 CRC64;
MASISTTTWL YRGQVCTDSG KSSNCIVQRR VKCGFPLKTL HAGITSRDRS LRHCIKCKKE
DGDGDVSEGS KKSEEGFEYV TVERHPYHSY MDSTSGKLEP ASGARASIPG EDYWPEGTSS
RVRAARAPQP AGESSSFPSY GKNPGSRRKK NRKATEENVT VETNDEVSDS EDSSEEEEND
SSDGFVTYKN EFEREEEETG FELDKKLGRP HPFIDPTKKK QIEKTLTSDE SWWNWRKPEK
EQWSRWQRRR PDVETVFLKA MAETGQVKLY GEEPTLTETS LYRARRHLFK EERLQAERER
LAKEGPMAFY SEWVKAWKRD TSREAVQKHF EETGEDENTQ LIEMFSHQTD REYRIMMGTD
IRIKRDPLAM RMREDQIKQI WGGDPVYPTI NYIQDPNAVM DFRGPDFHEP TPNMLSYLKE
NGKVISREMH EALLTKEKTE QLEVPDMDDA MAQAVDIGEN DDDEDDADVE KDDEKVPRNW
SVLKETPELR TAKPKPKKEG RMSLDEAVDD AENLTDFLMD FEEETDP
