PTA12_MAIZE
ID PTA12_MAIZE Reviewed; 555 AA.
AC B4FZ81; B6SV93;
DT 25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT 23-SEP-2008, sequence version 1.
DT 03-AUG-2022, entry version 68.
DE RecName: Full=Protein PLASTID TRANSCRIPTIONALLY ACTIVE 12, chloroplastic {ECO:0000305};
DE Short=ZmpTAC12 {ECO:0000303|PubMed:24246379};
DE Flags: Precursor;
GN Name=PTAC12 {ECO:0000303|PubMed:24246379};
GN ORFNames=ZEAMMB73_Zm00001d043325 {ECO:0000312|EMBL:ONM37552.1};
OS Zea mays (Maize).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; PACMAD clade;
OC Panicoideae; Andropogonodae; Andropogoneae; Tripsacinae; Zea.
OX NCBI_TaxID=4577;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. B73;
RX PubMed=19965430; DOI=10.1126/science.1178534;
RA Schnable P.S., Ware D., Fulton R.S., Stein J.C., Wei F., Pasternak S.,
RA Liang C., Zhang J., Fulton L., Graves T.A., Minx P., Reily A.D.,
RA Courtney L., Kruchowski S.S., Tomlinson C., Strong C., Delehaunty K.,
RA Fronick C., Courtney B., Rock S.M., Belter E., Du F., Kim K., Abbott R.M.,
RA Cotton M., Levy A., Marchetto P., Ochoa K., Jackson S.M., Gillam B.,
RA Chen W., Yan L., Higginbotham J., Cardenas M., Waligorski J., Applebaum E.,
RA Phelps L., Falcone J., Kanchi K., Thane T., Scimone A., Thane N., Henke J.,
RA Wang T., Ruppert J., Shah N., Rotter K., Hodges J., Ingenthron E.,
RA Cordes M., Kohlberg S., Sgro J., Delgado B., Mead K., Chinwalla A.,
RA Leonard S., Crouse K., Collura K., Kudrna D., Currie J., He R.,
RA Angelova A., Rajasekar S., Mueller T., Lomeli R., Scara G., Ko A.,
RA Delaney K., Wissotski M., Lopez G., Campos D., Braidotti M., Ashley E.,
RA Golser W., Kim H., Lee S., Lin J., Dujmic Z., Kim W., Talag J., Zuccolo A.,
RA Fan C., Sebastian A., Kramer M., Spiegel L., Nascimento L., Zutavern T.,
RA Miller B., Ambroise C., Muller S., Spooner W., Narechania A., Ren L.,
RA Wei S., Kumari S., Faga B., Levy M.J., McMahan L., Van Buren P.,
RA Vaughn M.W., Ying K., Yeh C.-T., Emrich S.J., Jia Y., Kalyanaraman A.,
RA Hsia A.-P., Barbazuk W.B., Baucom R.S., Brutnell T.P., Carpita N.C.,
RA Chaparro C., Chia J.-M., Deragon J.-M., Estill J.C., Fu Y., Jeddeloh J.A.,
RA Han Y., Lee H., Li P., Lisch D.R., Liu S., Liu Z., Nagel D.H., McCann M.C.,
RA SanMiguel P., Myers A.M., Nettleton D., Nguyen J., Penning B.W.,
RA Ponnala L., Schneider K.L., Schwartz D.C., Sharma A., Soderlund C.,
RA Springer N.M., Sun Q., Wang H., Waterman M., Westerman R., Wolfgruber T.K.,
RA Yang L., Yu Y., Zhang L., Zhou S., Zhu Q., Bennetzen J.L., Dawe R.K.,
RA Jiang J., Jiang N., Presting G.G., Wessler S.R., Aluru S.,
RA Martienssen R.A., Clifton S.W., McCombie W.R., Wing R.A., Wilson R.K.;
RT "The B73 maize genome: complexity, diversity, and dynamics.";
RL Science 326:1112-1115(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=18937034; DOI=10.1007/s11103-008-9415-4;
RA Alexandrov N.N., Brover V.V., Freidin S., Troukhan M.E., Tatarinova T.V.,
RA Zhang H., Swaller T.J., Lu Y.-P., Bouck J., Flavell R.B., Feldmann K.A.;
RT "Insights into corn genes derived from large-scale cDNA sequencing.";
RL Plant Mol. Biol. 69:179-194(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. B73;
RX PubMed=19936069; DOI=10.1371/journal.pgen.1000740;
RA Soderlund C., Descour A., Kudrna D., Bomhoff M., Boyd L., Currie J.,
RA Angelova A., Collura K., Wissotski M., Ashley E., Morrow D., Fernandes J.,
RA Walbot V., Yu Y.;
RT "Sequencing, mapping, and analysis of 27,455 maize full-length cDNAs.";
RL PLoS Genet. 5:E1000740-E1000740(2009).
RN [4]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=24246379; DOI=10.1104/pp.113.228726;
RA Williams-Carrier R., Zoschke R., Belcher S., Pfalz J., Barkan A.;
RT "A major role for the plastid-encoded RNA polymerase complex in the
RT expression of plastid transfer RNAs.";
RL Plant Physiol. 164:239-248(2014).
RN [5]
RP FUNCTION, IDENTIFICATION BY MASS SPECTROMETRY, SUBUNIT, AND SUBCELLULAR
RP LOCATION.
RX PubMed=25599833; DOI=10.1111/nph.13248;
RA Pfalz J., Holtzegel U., Barkan A., Weisheit W., Mittag M., Pfannschmidt T.;
RT "ZmpTAC12 binds single-stranded nucleic acids and is essential for
RT accumulation of the plastid-encoded polymerase complex in maize.";
RL New Phytol. 206:1024-1037(2015).
CC -!- FUNCTION: Required for the activity of the plastid-encoded RNA
CC polymerase (PEP) and full expression of genes transcribed by PEP
CC (PubMed:24246379, PubMed:25599833). Required for the proper build-up
CC and formation of the PEP-complex. Binds single-stranded (ss) DNA and
CC RNA, but not double-stranded (ds) DNA (PubMed:25599833).
CC {ECO:0000269|PubMed:24246379, ECO:0000269|PubMed:25599833}.
CC -!- SUBUNIT: Component of the plastid-encoded plastid RNA polymerase (PEP)
CC complex. {ECO:0000269|PubMed:25599833}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma
CC {ECO:0000269|PubMed:25599833}. Nucleus {ECO:0000269|PubMed:25599833}.
CC -!- DISRUPTION PHENOTYPE: Pale yellow-green leaf phenotype. Reduced levels
CC of plastid ribosomes and defects in plastid mRNA metabolism.
CC {ECO:0000269|PubMed:24246379}.
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DR EMBL; CM007649; ONM37552.1; -; Genomic_DNA.
DR EMBL; EU956658; ACG28776.1; -; mRNA.
DR EMBL; EU956938; ACG29056.1; -; mRNA.
DR EMBL; BT042419; ACF87424.1; -; mRNA.
DR RefSeq; NP_001143074.1; NM_001149602.1.
DR RefSeq; XP_008672559.1; XM_008674337.1.
DR AlphaFoldDB; B4FZ81; -.
DR STRING; 4577.GRMZM5G897926_P02; -.
DR PaxDb; B4FZ81; -.
DR PRIDE; B4FZ81; -.
DR EnsemblPlants; Zm00001eb152010_T001; Zm00001eb152010_P001; Zm00001eb152010.
DR EnsemblPlants; Zm00001eb152010_T002; Zm00001eb152010_P002; Zm00001eb152010.
DR EnsemblPlants; Zm00001eb152010_T003; Zm00001eb152010_P003; Zm00001eb152010.
DR EnsemblPlants; Zm00001eb152010_T004; Zm00001eb152010_P004; Zm00001eb152010.
DR GeneID; 100275546; -.
DR Gramene; Zm00001eb152010_T001; Zm00001eb152010_P001; Zm00001eb152010.
DR Gramene; Zm00001eb152010_T002; Zm00001eb152010_P002; Zm00001eb152010.
DR Gramene; Zm00001eb152010_T003; Zm00001eb152010_P003; Zm00001eb152010.
DR Gramene; Zm00001eb152010_T004; Zm00001eb152010_P004; Zm00001eb152010.
DR KEGG; zma:100275546; -.
DR eggNOG; ENOG502QS8P; Eukaryota.
DR HOGENOM; CLU_037383_0_0_1; -.
DR OMA; YSYMDST; -.
DR OrthoDB; 586120at2759; -.
DR Proteomes; UP000007305; Chromosome 3.
DR ExpressionAtlas; B4FZ81; baseline and differential.
DR GO; GO:0009507; C:chloroplast; IBA:GO_Central.
DR GO; GO:0009570; C:chloroplast stroma; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000427; C:plastid-encoded plastid RNA polymerase complex; IEA:EnsemblPlants.
DR GO; GO:0003697; F:single-stranded DNA binding; IDA:UniProtKB.
DR GO; GO:0003727; F:single-stranded RNA binding; IDA:UniProtKB.
DR GO; GO:0042793; P:plastid transcription; IBA:GO_Central.
DR GO; GO:0090228; P:positive regulation of red or far-red light signaling pathway; IEA:EnsemblPlants.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0010468; P:regulation of gene expression; IMP:UniProtKB.
DR GO; GO:0009637; P:response to blue light; IEA:EnsemblPlants.
DR GO; GO:0010218; P:response to far red light; IEA:EnsemblPlants.
DR GO; GO:0010114; P:response to red light; IEA:EnsemblPlants.
DR InterPro; IPR034581; PTAC12.
DR PANTHER; PTHR35720; PTHR35720; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Nucleus; Plastid; Reference proteome; Transcription;
KW Transcription regulation; Transit peptide.
FT TRANSIT 1..58
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 59..555
FT /note="Protein PLASTID TRANSCRIPTIONALLY ACTIVE 12,
FT chloroplastic"
FT /id="PRO_0000441847"
FT REGION 80..100
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 115..167
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 468..541
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 519..541
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 39
FT /note="S -> G (in Ref. 2; ACG28776/ACG29056)"
FT /evidence="ECO:0000305"
FT CONFLICT 63
FT /note="D -> N (in Ref. 2; ACG28776/ACG29056)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 555 AA; 63297 MW; C8D43C47F3EC869C CRC64;
MASCYNPWRL FPGMSTAVPA GPVTAPAHSR TCKSSKVFSA LPHRRGLLFL GTRRARIKCV
KDDSLHFDPS KIEPPPYSSY FDSTSGQLEP ASGARASIPG KEYWPEGTAA RVRAARAPAP
VGESAGMPSF GTKPGSRRRG YKEQVTSASG TEGAQTDDRK DGDEPDVAII GSGDDALEEI
KDSVDEYVIY ETPEEEELSE YDMDKMMGRP HPFIDPAKAM SLGEPKTSEE LWWHWRRKSQ
EEEMWSRWQR RRPDVDTVFA KAMAETGQIK IFGDHPSRTE AALAKTRRHL YKEERLEAEQ
RRLEEIGPIA YYSEWVEAYK NKDTSREAIQ KHFEETGEDE NVQLIKMFQH QTAGEYRIMM
GTDVRIQRDP LAMRMREDQI KQIWGGDPVY PTINYVQDPD EVIDYRGPEF HEPTPEVVPY
LMEHGIMITK EELYARLNEE REDVNQDITY IPEAKDPMAT AIDIGEHSYN EDSDDEDEDV
DKAAAQPQSL EDEEDDRDDV AEVEEKVNQN WSALKSTGQA EKPKEKSKKD EMTLKEAIDD
SENLTDFLMD FEETE
