PTAC6_ARATH
ID PTAC6_ARATH Reviewed; 328 AA.
AC Q9XI19;
DT 26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=PLASTID TRANSCRIPTIONALLY ACTIVE protein 6, chloroplastic {ECO:0000303|PubMed:16326926};
DE Short=pTAC6 {ECO:0000303|PubMed:16326926};
DE Flags: Precursor;
GN Name=PTAC6 {ECO:0000303|PubMed:16326926};
GN OrderedLocusNames=At1g21600 {ECO:0000312|Araport:AT1G21600};
GN ORFNames=F8K7.1 {ECO:0000312|EMBL:AAD41412.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia; TISSUE=Flower, and Silique;
RX PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA Shinozaki K.;
RT "Analysis of multiple occurrences of alternative splicing events in
RT Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL DNA Res. 16:155-164(2009).
RN [5]
RP FUNCTION, DISRUPTION PHENOTYPE, TISSUE SPECIFICITY, SUBUNIT, IDENTIFICATION
RP BY MASS SPECTROMETRY, SUBCELLULAR LOCATION, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=16326926; DOI=10.1105/tpc.105.036392;
RA Pfalz J., Liere K., Kandlbinder A., Dietz K.-J., Oelmueller R.;
RT "pTAC2, -6, and -12 are components of the transcriptionally active plastid
RT chromosome that are required for plastid gene expression.";
RL Plant Cell 18:176-197(2006).
RN [6]
RP IDENTIFICATION.
RX PubMed=21139083; DOI=10.1104/pp.110.168120;
RA Bryant N., Lloyd J., Sweeney C., Myouga F., Meinke D.;
RT "Identification of nuclear genes encoding chloroplast-localized proteins
RT required for embryo development in Arabidopsis.";
RL Plant Physiol. 155:1678-1689(2011).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=24272250; DOI=10.1093/pcp/pct165;
RA Akiyama K., Kurotani A., Iida K., Kuromori T., Shinozaki K., Sakurai T.;
RT "RARGE II: an integrated phenotype database of Arabidopsis mutant traits
RT using a controlled vocabulary.";
RL Plant Cell Physiol. 55:E4-E4(2014).
RN [8]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, AND INTERACTION WITH
RP MTERF5.
RC STRAIN=cv. Columbia;
RX PubMed=31128276; DOI=10.1016/j.molp.2019.05.007;
RA Ding S., Zhang Y., Hu Z., Huang X., Zhang B., Lu Q., Wen X., Wang Y.,
RA Lu C.;
RT "mTERF5 acts as a transcriptional pausing factor to positively regulate
RT transcription of chloroplast psbEFLJ.";
RL Mol. Plant 12:1259-1277(2019).
CC -!- FUNCTION: Essential protein involved in plastid gene expression
CC (PubMed:16326926, PubMed:31128276). Recruited by MTERF5 to the
CC transcriptionally paused region of psbEFLJ (PubMed:31128276). Promotes
CC leaf greening (PubMed:24272250). {ECO:0000269|PubMed:16326926,
CC ECO:0000269|PubMed:24272250, ECO:0000269|PubMed:31128276}.
CC -!- SUBUNIT: Subunit of the plastid-encoded RNA polymerase (PEP) complex
CC (PubMed:16326926, PubMed:31128276). Interacts with MTERF5
CC (PubMed:31128276). {ECO:0000269|PubMed:16326926,
CC ECO:0000269|PubMed:31128276}.
CC -!- INTERACTION:
CC Q9XI19; O23160: MYB73; NbExp=3; IntAct=EBI-7890412, EBI-25506855;
CC Q9XI19; Q84MC7: PYL9; NbExp=3; IntAct=EBI-7890412, EBI-2349513;
CC Q9XI19; Q84MB2: TIFY8; NbExp=3; IntAct=EBI-7890412, EBI-4426557;
CC Q9XI19; Q5CCK4: VAL2; NbExp=3; IntAct=EBI-7890412, EBI-15193683;
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000305|PubMed:16326926, ECO:0000305|PubMed:31128276}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in rosette leaves, stems and
CC flowers, and, to a lower extent, in roots and cauline leaves.
CC {ECO:0000269|PubMed:16326926}.
CC -!- DISRUPTION PHENOTYPE: Lethal without exogenous carbon sources
CC (PubMed:16326926). Reduced expression of plastid-encoded genes with
CC plastid-encoded multimeric promoters (PEP) (PubMed:16326926,
CC PubMed:31128276). Strongly reduced presence of both plastid- and
CC nuclear-encoded soluble polypeptides and polypeptides associated with
CC the thylakoid membrane in chloroplasts (PubMed:16326926). Albino plants
CC unable to produce primary leaves white cotyledons that fails to
CC accumulate chlorophyll (Chl) even in low light, thus leading to high
CC nonphotochemical quenching (NPQ) (PubMed:16326926, PubMed:24272250).
CC Abnormal chloroplasts which fail to contain grana thylakoids that are
CC replaced by oval-shaped vesicles surrounded by plastoglobuli
CC (PubMed:16326926). Starch accumulates only in old leaves
CC (PubMed:16326926). {ECO:0000269|PubMed:16326926,
CC ECO:0000269|PubMed:24272250, ECO:0000269|PubMed:31128276}.
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DR EMBL; AC007727; AAD41412.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30122.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE30123.1; -; Genomic_DNA.
DR EMBL; AF324715; AAG40066.1; -; mRNA.
DR EMBL; AF326894; AAG41476.1; -; mRNA.
DR EMBL; AF446886; AAL38619.1; -; mRNA.
DR EMBL; AY052666; AAK96570.1; -; mRNA.
DR EMBL; AY113873; AAM44921.1; -; mRNA.
DR EMBL; AK317001; BAH19696.1; -; mRNA.
DR PIR; H86348; H86348.
DR RefSeq; NP_001031076.1; NM_001035999.3.
DR RefSeq; NP_564144.1; NM_102009.3.
DR AlphaFoldDB; Q9XI19; -.
DR IntAct; Q9XI19; 5.
DR MINT; Q9XI19; -.
DR STRING; 3702.AT1G21600.1; -.
DR PaxDb; Q9XI19; -.
DR PRIDE; Q9XI19; -.
DR ProteomicsDB; 177345; -.
DR EnsemblPlants; AT1G21600.1; AT1G21600.1; AT1G21600.
DR EnsemblPlants; AT1G21600.2; AT1G21600.2; AT1G21600.
DR GeneID; 838761; -.
DR Gramene; AT1G21600.1; AT1G21600.1; AT1G21600.
DR Gramene; AT1G21600.2; AT1G21600.2; AT1G21600.
DR KEGG; ath:AT1G21600; -.
DR Araport; AT1G21600; -.
DR TAIR; locus:2026972; AT1G21600.
DR eggNOG; ENOG502QWJH; Eukaryota.
DR HOGENOM; CLU_060207_0_0_1; -.
DR InParanoid; Q9XI19; -.
DR OMA; IFLTKHY; -.
DR OrthoDB; 1052072at2759; -.
DR PhylomeDB; Q9XI19; -.
DR PRO; PR:Q9XI19; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9XI19; baseline and differential.
DR GO; GO:0009507; C:chloroplast; IC:UniProtKB.
DR GO; GO:0042644; C:chloroplast nucleoid; HDA:TAIR.
DR GO; GO:0000427; C:plastid-encoded plastid RNA polymerase complex; IDA:UniProtKB.
DR GO; GO:0042793; P:plastid transcription; IDA:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IMP:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:UniProtKB.
DR InterPro; IPR044710; PTAC6.
DR PANTHER; PTHR35994; PTHR35994; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Plastid; Reference proteome; Transcription;
KW Transcription regulation; Transit peptide.
FT TRANSIT 1..59
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 60..328
FT /note="PLASTID TRANSCRIPTIONALLY ACTIVE protein 6,
FT chloroplastic"
FT /id="PRO_0000449048"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 328 AA; 37377 MW; 1E0DB8EE79E6C8FB CRC64;
MASSAASPSL SLLSFTSKPP YPSGSQRLFA SFRTDGLFAP LTLKSRRGRG IVVKVDDVDA
DGGGADEYDM DDEEVEEVDN KKDYDVEYDP LAAAIAAAGG GGDGDIAFVQ SKSFISTQGW
DSDMVVDYRI NEDEFHKLSL MDCDFFIRKP PDPDNDVYDF REMYVTPPDT DIYSIPRVLA
PMPQKYIRCA MSDYGCYNVT EPPIDAPRDP LYKSEREISK VFLTKHYRNR RTNDPEFVLD
LEEIYVIDSK TKSITRARVL VTVPGGRKRD RKDDLLVIRD NGTSFKIIHV GERDDPTTVI
EREEWTKTRE DMEKHLRKLR DFSVSNWF
